A simple solution to the problem of selective detection of phosphate and arsenate by the molybdenum blue method.

We report a simple and fast method for the quantification of both phosphate and arsenate in water using the molybdenum blue method. The method does not require established pre-treatment procedures for physically separating arsenate and phosphate or reducing the arsenate to arsenite. In our method, the heteropolymolybdate ions in the solution are precipitated and collected on a membrane that is transparent in both the visible and infrared regions of the spectrum. The phosphate is determined by recording a visible spectrum in transmission mode through the membrane. This membrane is then air-dried, and then an infrared spectrum is recorded through the membrane in transmission mode. The concentration of arsenate is then determined from the intensity of an As-O band positioned at 879 cm-1 in the infrared spectrum. Using this method, a detection limit of 0.86 µg L-1 phosphate and 13.9 µg L-1 arsenate in water was achieved. Matrix spikes on environmental samples gave a 108% recovery of arsenate and a 105% recovery of phosphate over a dynamic range of 25-250 µg L-1 of arsenate and phosphate in the sample.

A solventless method for detecting trace level phosphate and arsenate in water using a transparent membrane and visible spectroscopy.

The molybdenum blue method is the American Public Health Association (APHA) approved method for the detection and quantification of phosphate in water. The standard molybdenum blue method, APHA 4500 PE has a detection limit of 30 µgL-1 phosphate (10 µgL-1 phosphorus) in freshwater with a 5 cm cuvette. To further lower the detection limit to sub µgL-1 levels, we have developed a simple, fast, and solventless method for conversion of phosphate present in solution to a solid for quantification by Visible spectroscopy. The process converts the anionic heteropolymolybdate ions into a solid colloidal precipitate by charge neutralization with the cationic surfactant cetyltrimethylammonium bromide (CTAB), and the precipitate is then captured on a Visible transparent membrane. A Visible spectrum is then recorded in transmission mode through the membrane and the concentration of the phosphate is determined from the intensity of a band cantered at 700 nm. Using this method, the detection limit for phosphate in water is lowered to 0.64 µgL-1. The approach has also been extended to detect arsenate in water with a detection limit of 4.8 µgL-1 arsenate. . The method is also used to investigate real matrices with accuracy that matches the standard APHA method for detection of phosphate in water.

Vildagliptin vs sulfonylurea in Indian Muslim diabetes patients fasting during Ramadan.

AIM: To compare the use of vildagliptin and sulfonylurea with or without metformin in Indian Muslim patients with type 2 diabetes mellitus, fasting during Ramadan. METHODS: This was a 4-wk, multicenter, non-interventional, open-label, observational study. Incidence of hypoglycemic events (HEs), adverse events, and changes in glycosylated hemoglobin A1c (HbA1c), fasting plasma glucose, postprandial plasma glucose and body weight were measured pre- and post-Ramadan. RESULTS: Totally, 97 patients were recruited and all completed the study (vildagliptin group, n = 55; sulfonylurea group, n = 42). HEs were reported in low frequencies in both the vildagliptin and the sulfonylurea groups [0 vs 2 (4.8%) patients, respectively]. Interestingly, HbA1c reduced by -0.43% (-4.71 mmol/mol) in the vildagliptin group [8.75% (72.10 mmol/mol) to 8.32% (67.38 mmol/mol), P = 0.009] while in the sulfonylurea group there was a small increase by 0.01% [0.08 mmol/mol; 8.64% (70.92 mmol/mol) to 8.65% (71.00 mmol/mol), P = 0.958]. Higher percentage of vildagliptin-treated patients achieved HbA1c < 7.0% (< 53 mmol/mol) compared with sulfonylurea (16.4% vs 4.8%). Mean decrease in the body weight was 1.2 kg and 0.03 kg, respectively (P < 0.001). Both treatment groups were well tolerated during Ramadan. CONCLUSION: Vildagliptin is an attractive treatment option for Indian patients with type 2 diabetes mellitus who are fasting during Ramadan.

Superstitions regarding health problems in different ethnic groups in Karachi.

OBJECTIVE: To find out the superstitions regarding health problems in different ethnic groups, their implications over the socio-economic development of that group and to what extent can those superstitions be related to their level of literacy. METHODS: The study was a questionnaire-based survey, 20 subjects from each ethnic group were selected by cluster sampling of residential areas where that particular group has its highest concentration, making a total of 100 subjects. RESULTS: It was found that most people (73%) do have some superstitious beliefs. Fifty percent of people believe in them as a part of culture and tradition, another 25% got them from their elders. No significant difference was found between different racial groups (p value = 0.9). According to literacy rate, 73.5% of literate community and 94.1% illiterate community were found to have superstitions. The occupation of the breadwinner of family didn't have a significant impact over the belief in superstitions (p value = 0.6). CONCLUSION: Majority of our population believes in superstitions, which are more common in illiterates. These superstitions not only predict health seeking behaviour of a person but also play a major role in shaping the response of a community to any health intervention program. Without the knowledge of these superstitions, effective community participation cannot be achieved.