ABSTRACT
AIM: To determine sensitivity of tumor plasmocytes in vitro to cytostatic drugs (prednisolone, alkeran belustin, vincristine, rubomycin, doxorubicin, cytarabin, methotrexate, cysplatin, etoposide). MATERIAL AND METHODS: The sensitivity was measured with DISC method in 12 patients with multiple myeloma (MM) in two groups: resistant and responsive to induction polychemotherapy (PCT). RESULTS: The groups appeared significantly different by lowering of pathological paraprotein concentration (PIg): by 7.4 +/- 2.5% and 32.5 +/- 3.7%, respectively (p < 0.05). The resistance to the drugs was higher in the resistant patients than in the responders (0.7 +/- 0.28 versus 0.4 +/- 0.02, p < 0.05). PCT schemes of resistant patients contained 65.0 +/- 2.3% of ineffective drugs. In the responders the percentage was 35.7 +/- 5.3% (p < 0.05). CONCLUSION: The relationship exists between resistance of tumor plasmocytes to drugs in vitro and clinical findings.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Multiple Myeloma/pathology , Plasma Cells/drug effects , Adult , Aged , Antineoplastic Agents/therapeutic use , Apoptosis/drug effects , Drug Resistance , Female , Humans , Male , Middle Aged , Multiple Myeloma/drug therapy , Plasma Cells/pathology , Tumor Cells, Cultured/drug effectsABSTRACT
A modified method was developed to isolate lactoferrin from women's colostrum by means of ion-exchange chromatography on a Servacel CM-52 column and gel filtration on a Toyoperal HW-52 column. These changes simplified the basic method and decreased the length of the procedure without affecting the biological activity and purity of the preparation. The modified method can be recommended to produce lactoferrin suitable for the use as a pharmaceutical preparation and nutritional additive to milk substitutes.
Subject(s)
Chromatography, Ion Exchange/methods , Lactoferrin/isolation & purification , Polymers , Female , Humans , Milk, HumanABSTRACT
A new fluorometric method for detection of amide protein groups is proposed. The method has a number of advantages over the routine phenol hypochlorite method, this permitting its wider application in clinical and laboratory practice. The suggested method may be used for direct detection of ammonium in biological samples.
Subject(s)
Amides/analysis , Fluorometry , Proteins/chemistry , Animals , Diabetes Mellitus, Experimental/metabolism , Humans , Insulin/analysis , Insulin/metabolism , Phenols , Quaternary Ammonium Compounds/analysisSubject(s)
Leukemia, Myelomonocytic, Acute/complications , Opportunistic Infections/etiology , Tuberculosis, Pulmonary/etiology , Acute Disease , Adolescent , Diagnosis, Differential , Fatal Outcome , Humans , Leukemia, Myelomonocytic, Acute/diagnosis , Male , Opportunistic Infections/diagnosis , Tuberculosis, Pulmonary/diagnosisABSTRACT
HLA antigens class 1 have been investigated in Russian patients with multiple myeloma. Compared to controls, myeloma patients demonstrated more frequent occurrence of antigens A11 and B7. Antigen A11 was more common for males and subjects with early response to treatment. Antigen B7 was more frequent in females with the slow response. These correlations are significant without correction on the number of the antigens studied. Association heterogeneity in males and females, in different therapy responders gives grounds for a differential approach to myeloma pathogenesis, choice of drugs, prognosis of survival.
Subject(s)
Histocompatibility Antigens Class I/blood , Multiple Myeloma/immunology , Adult , Aged , Chi-Square Distribution , Disease Susceptibility , Female , Humans , Male , Middle Aged , Multiple Myeloma/ethnology , Russia/epidemiology , Sex DistributionABSTRACT
An immunological phenotype of blood lymphocytes and proliferative activity of tumor cells were studied using a panel of monoclonal antibodies to lymphocyte differential antigens and tracer 3H-thymidine, respectively, in 84 patients with multiple myeloma. CD-19 and CD38 antigens were identified as markers for circulating tumor lymphocytes. The count of CD38+ and CD71+lymphocytes correlated with the count of marrow plasmocytes including 3H-thymidine in the disease recurrence. Proliferative parameters of blood lymphocytes, plasmocytes and lymphocytes of the bone marrow were identical being, consequently, elements of the same tumor clone.
Subject(s)
Immunophenotyping , Lymphocytes/immunology , Multiple Myeloma/immunology , Adult , Aged , Antigens, CD/immunology , Autoradiography , Bone Marrow/immunology , Bone Marrow/pathology , Cell Division/immunology , Female , Humans , Lymphocytes/pathology , Male , Middle Aged , Multiple Myeloma/pathology , Multiple Myeloma/therapy , Plasma Cells/immunology , Plasma Cells/pathology , Recurrence , Remission InductionABSTRACT
The proliferative response and in vitro immunoglobulin production induced polyclonal activators were studied in 12 patients with multiple myeloma (MM) and in 15 healthy individuals. The spontaneous production of IgM was decreased. PWM-induced immunoglobulin production of the peripheral blood lymphocytes was the same.
