ABSTRACT
Rosmarinic acid (RA) is a natural phenylpropanoid with numerous pharmacological activities. Because of limited studies of the effects of RA action in gastric cancer cells we examined how 100 and 200⯵M acid influences MMPs, TIMPs, collagen, MUC1 and specific sugar antigens in gastric adenocarcinoma CRL-1739 cells. We revealed inhibitory effect of RA on MMP-9 activity what was correlated with increased collagen type I expression, main ECM substrate degraded by MMPs. Tissue inhibitor of MMPs, TIMP-1 but not TIMP-2 was significantly decreased on the protein level and increased on mRNA level by RA action what can suggest TIMP-1 independent inhibitory action of an acid on MMP-9 activity. Glycosylation of gastric cancer proteins was also effected by RA. ELISA tests revealed inhibitory effect of an acid on Tn antigen in cell lysates and culture supernatant and on T antigen in cell lysates. RA inhibited also sialylated Tn antigen in protein of culture supernatant and sialyl T in cell lysates. Extracellular domain of MUC1 mucin, main carrier of Tn and T antigens was significantly inhibited by higher dose of RA. The data suggest potential usefulness of RA as a complementary agent supporting chemotherapy in cancer treatment.
Subject(s)
Cinnamates/pharmacology , Collagen/metabolism , Depsides/pharmacology , Matrix Metalloproteinases/metabolism , Mucin-1/metabolism , Stomach Neoplasms/metabolism , Tissue Inhibitor of Metalloproteinases/metabolism , Antigens/metabolism , Cell Line, Tumor , Cinnamates/chemistry , Depsides/chemistry , Glycosylation , Humans , Stomach Neoplasms/enzymology , Stomach Neoplasms/pathology , Rosmarinic AcidABSTRACT
Diabetes mellitus is a chronic metabolic disease which is a serious global problem. In 2010 an estimated 285 million people had diabetes and within the next 20 years this value is expected to almost double. Many antidiabetic therapies focus on improving insulin sensitivity, increasing insulin production, and/or decreasing the level of blood glucose. Although a number of synthetic medicines are available, drugs of natural origin have aroused great interest. Triterpenes seem to demonstrate adequate properties. Many experiments have shown that these compounds have several antidiabetic mechanisms. They can inhibit enzymes involved in glucose metabolism, prevent the development of insulin resistance and normalize plasma glucose and insulin levels. These natural compounds, in contrast to synthetic drugs, apart from producing a hypoglycemic effect have also been found to manifest hypolipidemic and anti-obesity activity. Triterpenes are also promising agents in the prevention of diabetic complications. They have strong antioxidant activity and inhibit the formation of advanced glycation end products, implicated in the pathogenesis of diabetic nephropathy, embryopathy, neuropathy or impaired wound healing. Until now very few clinical studies have been concerned with the application of triterpenes in treating diabetes. However, due to their great therapeutic potential, these compounds deserve special attention.
ABSTRACT
Methanolic extracts from inflorescences and leaves of five Cirsium species: C. arvense, C. oleraceum, C. palustre, C. rivulare and C. vulgare and CHCl3, Et2O, EtOAc, n-BuOH fractions obtained from those extracts were investigated for their antioxidant activity. The antioxidant activity measurement, expressed as Total Antioxidant Status, ranged from 0.98 to 2.71 mM/l. The total phenolic content of the samples varied from 12 to 804 mg/g. There was a positive correlation between the total phenolic content and antioxidant activity for MeOH extracts and EtOAc fractions (R2=0.95, 0.8 respectively).
Subject(s)
Antioxidants/pharmacology , Cirsium , Phytotherapy , Plant Extracts/pharmacology , Antioxidants/administration & dosage , Antioxidants/therapeutic use , Chromans/chemistry , Humans , Medicine, Traditional , Phenols/administration & dosage , Phenols/pharmacology , Phenols/therapeutic use , Plant Extracts/administration & dosage , Plant Extracts/therapeutic use , PolandABSTRACT
Three flavonol glycosides, isorhamnetin 3-O-beta-D-galactopyranoside, isorhamnetin 3-O-beta-D-(6"-acetyl)-galactopyranoside and kaempferol 3-O-beta-D-glucopyranoside were isolated from the flowers of Bellis perennis.
Subject(s)
Asteraceae , Flavonoids/chemistry , Flavonols , Glycosides/chemistry , Kaempferols , Phytotherapy , Plant Extracts/chemistry , Quercetin/analogs & derivatives , Humans , Plant Structures , Quercetin/chemistryABSTRACT
The qualitative and quantitative analysis of flavonoids in the flowers and leaves of naturally growing B. perennis and in the flowers of cultivated variations 'rosea' and 'rubra' were carried out. The qualitative analysis was carried out by TLC and compared with flavonoid compounds isolated from flowers of common daisy. The quantitative determinations were carried out by Christ-Müller's method and by HPLC after acid hydrolysis. Similar flavonoids were found in all the flowers, while differences were noted in the flavonoid composition of the leaves. The flavonoid contents were higher in the flowers than in the leaves.
Subject(s)
Asteraceae/chemistry , Flavonoids/analysis , Flavonols , Kaempferols , Quercetin/analogs & derivatives , Apigenin , Chromatography, Thin Layer , Plant Shoots/chemistry , Quercetin/analysisABSTRACT
Two next apigenin glycosides, apigenin 7-O-beta-D-glucuronide [III], apigenin 7-O-beta-D-glucoside [IV] were isolated from the flowers of Bellis perennis L. Their structures were determined by means of chemical and spectroscopic methods.
