Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Microbiol ; 204(4): 219, 2022 Mar 25.
Article in English | MEDLINE | ID: mdl-35333980

ABSTRACT

During past few decades, endophytes have gained importance due their ability to produce bioactive compounds. Many medicinal plants are being exploited for the endophytic isolation to obtain drugs of interest. This study explored the fungal endophytes of Olive (Olea europaea L.) stem from which Fusarium redolens was selected for investigation of bioactive potential. The endophyte was identified using morphological characteristics and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. The GCMS analysis of the crude extract yielded chrysophanol and fumaric acid. The culture filtrate of ethyl acetate extract showed significant cytotoxic potential against HepG2 cells, respectively. Furthermore, the screening of antioxidant potential of the ethyl acetate fungal extract using DPPH scavenging assay showed that Fusarium redolens extract exhibited potential activity with a significant EC50 value of 144.7 µg/mL.


Subject(s)
Fusarium , Olea , Endophytes , Fungi/genetics
2.
Appl Biochem Biotechnol ; 163(5): 577-91, 2011 Mar.
Article in English | MEDLINE | ID: mdl-20730507

ABSTRACT

A thermotolerant Aspergillus fumigatus strain isolated from composting pile of mixed industrial waste was found to produce a spectrum of cellulase and hemicellulases when cultured on rice straw solidified substrate. The two-dimensional electrophoresis (2DE) resolved the secretome into 57 distinct protein spots. The zymograms developed against 2DE gels identified the presence of three ß-glucosidases and five CBHI/EGI isoforms in the secretome. The peptide mass fingerprinting of 17 protein spots by liquid chromatography mass spectrometry characterized the secretome into different glycosyl hydrolase families. The enzyme cocktail produced by A. fumigatus was capable of efficient hydrolysis of alkali pretreated rice straw (at 7% and 10% w/v) resulting in 95% and 91% saccharification, respectively.


Subject(s)
Alkalies/chemistry , Aspergillus fumigatus/enzymology , Glycoside Hydrolases/metabolism , Oryza/chemistry , Cellulases/metabolism , Electrophoresis, Gel, Two-Dimensional , Hydrolysis , Protein Isoforms/metabolism
3.
Appl Biochem Biotechnol ; 162(2): 538-47, 2010 Sep.
Article in English | MEDLINE | ID: mdl-19779865

ABSTRACT

This study reports differential expression of endoglucanase (EG) and beta-glucosidase (betaG) isoforms of Aspergillus terreus. Expression of multiple isoforms was observed, in presence of different carbon sources and culture conditions, by activity staining of poly acrylamide gel electrophoresis gels. Maximal expression of four EG isoforms was observed in presence of rice straw (28 U/g DW substrate) and corn cobs (1.147 U/ml) under solid substrate and shake flask culture, respectively. Furthermore, the sequential induction of EG isoforms was found to be associated with the presence of distinct metabolites (monosaccharides/oligosaccharides) i.e., xylose (X), G(1), G(3) and G(4) as well as putative positional isomers (G(1)/G(2), G(2)/G(3)) in the culture extracts sampled at different time intervals, indicating specific role of these metabolites in the sequential expression of multiple EGs. Addition of fructose and cellobiose to corn cobs containing medium during shake flask culture resulted in up-regulation of EG activity, whereas addition of mannitol, ethanol and glycerol selectively repressed the expression of three EG isoforms (Ia, Ic and Id). The observed regulation profile of betaG isoforms was distinct when compared to EG isoforms, and addition of glucose, fructose, sucrose, cellobiose, mannitol and glycerol resulted in down-regulation of one or more of the four betaG isoforms.


Subject(s)
Aspergillus/chemistry , Cellulase/analysis , beta-Glucosidase/analysis , Aspergillus/metabolism , Cellulase/metabolism , Down-Regulation , Isoenzymes/analysis , Isoenzymes/metabolism , Substrate Specificity , Up-Regulation , beta-Glucosidase/metabolism
4.
J Biotechnol ; 122(4): 494-510, 2006 Apr 20.
Article in English | MEDLINE | ID: mdl-16375985

ABSTRACT

The aryl tetralin lignans are synthesized by Podophyllum sps. and are in great demand worldwide due to their use in synthesis of topoisomerase inhibitors. However, the sustained production of these aryl tetralin lignans requires large-scale harvesting from the natural environments, which has resulted in the plant-endangered status. In view of the difficulties in their total chemical synthesis, cultivation and failure of metabolic engineering approaches, there is a need to search for alternative sources of production of aryl tetralin lignans. We unequivocally established the methodology for isolation, identification, and characterization of a novel fungal endophyte (Trametes hirsuta) that produces aryl tetralin lignans consistently as shown by HPLC, LC-MS, LC/MS-MS and (1)H NMR. The lignans produced by the microorganism are biologically active, and exhibit potent antioxidant, anticancer and radioprotective properties. This strategy promises to improve the production of these therapeutically important compounds at lower costs.


Subject(s)
Fungi/isolation & purification , Lignans/chemistry , Podophyllotoxin/chemistry , Tetrahydronaphthalenes/chemistry , Cell Fractionation , Chromatography, High Pressure Liquid , Fungi/cytology , Kinetics , Lipid Peroxidation/radiation effects , Models, Biological , Plant Cells , Plant Structures/cytology , Plant Structures/metabolism , Plants/metabolism , Podophyllotoxin/metabolism , Podophyllum/cytology , Podophyllum/metabolism , Spectrometry, Mass, Electrospray Ionization
SELECTION OF CITATIONS
SEARCH DETAIL
...