ABSTRACT
The article presents the results of evaluation of laboratory diagnostic value of relationships between the rate of detection of fragments of genes of pathogenicity islands associated with E. coli pathogenicity in clinical strains of S. enteritidis, antibiotic resistance of cultures and clinical characteristics of salmonellosis in adults. The sample included 105 patients with salmonellosis to apply the genetic test to isolated strains of S. enteritidis. The E. coli fragments of genes of pathogenicity islands are detected in 102 out of 105 analyzed strains of S. enteritidis (97.1%). The fragments specific to hlyA E. coli are detected in various qualitative and quantitative combinations in 50 (49.0%) clinical strains of S. enteritidis, hlyB--in 54 (52.9%), sfaG--in 57 (55.9%) and sfaA--in 29 (28.4%). The correlation is established between presence of fragments of genes of pathogenicity islands in S.enteritidis associated with E. coli pathogenicity and resistance to ampicillin, doxycycline, cephasoline, chloramphenicol, carbenicillin. The dependence of disease severity from S. enteridis poly-resistance to antibiotics is established.
Subject(s)
Drug Resistance, Bacterial/genetics , Salmonella Infections/microbiology , Salmonella enteritidis/genetics , Salmonella enteritidis/pathogenicity , Adhesins, Escherichia coli/genetics , Adult , Bacterial Proteins/genetics , Carrier Proteins/genetics , Escherichia coli/genetics , Escherichia coli/pathogenicity , Escherichia coli Proteins/genetics , Female , Fimbriae Proteins/genetics , Genetic Markers , Genomic Islands/genetics , Hemolysin Proteins/genetics , Humans , Male , Salmonella enteritidis/isolation & purificationABSTRACT
AIM: To evaluate diagnostic value of lipopolysaccharide-binding protein (LBP) level in different infectious processes and dysbiosis. MATERIALS AND METHODS: Serum samples of patients with salmonellosis, urogenital chlamydiosis, community-acquired pneumonia, polypous rhinosinusitis, and bacterial vaginosis were studied. RESULTS: LBP level were lower in patients with salmonellosis compared to healthy persons and decreased with increasing severity of the disease. Higher levels of LBP were detected in patients with chlamydiosis. Direct correlation between LBP level and etiology and severity of community-acquired pneumonia was demonstrated. In patients with polypous sinusitis, LBP level correlated directly with duration of disease, and inversely--with duration of remission. Twofold increase of mean LBP blood concentration and its correlation with duration of dysbiosis in patients with bacterial vaginosis were revealed. CONCLUSION: Multidirectional modulation of antiendotoxin defense was observed in different pathological conditions.
Subject(s)
Acute-Phase Proteins , Carrier Proteins , Lipopolysaccharides/metabolism , Membrane Glycoproteins , Acute-Phase Proteins/analysis , Acute-Phase Proteins/biosynthesis , Adult , Carrier Proteins/analysis , Carrier Proteins/biosynthesis , Chlamydia/pathogenicity , Chlamydia Infections/blood , Chlamydia Infections/diagnosis , Community-Acquired Infections/blood , Community-Acquired Infections/diagnosis , Down-Regulation , Female , Humans , Male , Membrane Glycoproteins/analysis , Membrane Glycoproteins/biosynthesis , Middle Aged , Pneumonia/blood , Pneumonia/diagnosis , Salmonella/pathogenicity , Salmonella Infections/blood , Salmonella Infections/diagnosis , Sinusitis/blood , Sinusitis/diagnosis , Up-Regulation , Vaginosis, Bacterial/blood , Vaginosis, Bacterial/diagnosisABSTRACT
AIM: To assess prevalence of fragments of Escherichia coli pathogenicity islands in Salmonella enteritidis strains as well as to study clinical signs of disease caused by these strains in adults. MATERIALS AND METHODS: Ninety-six patients with salmonellosis were studied. Ninety strains of S. enteritidis were isolated and tested by PCR for the presence of genes associated with pathogenicity islands of E. coli: hlyA, hlyB, sfaG, and sfaA. RESULTS: It was determined that DNA fragments homologous to pathogenicity islands of E. coli were present in 87 (96.7%) of S. enteritidis clinical isolates. Disease caused by Salmonella strains which possess only sfaG was mostly mild--7 (33.3%), whereas strains which had sfaG with fragments of hlyA and/or hlyB caused severe disease--7 (50%). sfaA fragments were found mostly in combination with other genes. In such cases the disease was mostly severe--6 (42.8%). CONCLUSION: Correlation between presence of E. coli pathogenicity islands in Salmonella spp., their antibiotic resistance and severity of infection was established.
