Antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian medicinal plant Eremophila neglecta.

AIMS: To determine the antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian plant Eremophila neglecta. METHODS AND RESULTS: Antimicrobial activities of serrulatane compounds 8,19-dihydroxyserrulat-14-ene (1) and 8-hydroxyserrulat-14-en-19-oic acid (2) were tested against Gram-negative and Gram-positive bacteria including human and veterinary pathogens and some multidrug-resistant isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the compounds were determined by broth microdilution assay. Both compounds exhibited antibacterial activity against all Gram-positive test strains. They showed antimycobacterial activity against isolates of Mycobacterium fortuitum and Mycobacterium chelonae. Of the five Gram-negative bacteria tested, only Moraxella catarrhalis showed susceptibility to the compounds. Cytotoxic activities were tested in the Vero cell line. Compound 1 showed more activity than 2 in both antibacterial and cytotoxicity assays with cytotoxicity at concentrations similar to the MBC. CONCLUSIONS: Serrulatane compounds showed significant activity against medically important bacteria, with 1 exhibiting stronger antibacterial activity. However, they also displayed toxicity to mammalian cells. SIGNIFICANCE AND IMPACT OF THE STUDY: Serrulatanes are of interest as novel antibacterial compounds for use in biomedical applications; this study reports data obtained with a range of bacterial strains and mammalian cells, essential for assessing the capabilities and limitations of potential applicability of these compounds.

An estimation of the economic impact of contagious bovine pleuropneumonia in Africa.

Contagious bovine pleuropneumonia (CBPP) is a disease that causes high morbidity and mortality losses to cattle. The financial implications of these losses are of great significance to cattle owners. Control of CBPP is therefore important as a way to salvage the losses and increase the incomes of cattle owners. This study estimated the economic cost of CBPP and the benefits of its control in twelve sub-Saharan African countries using a spreadsheet economic model developed in Microsoft Excel. The value of morbidity and mortality losses was estimated at 30 million euros (2.5 million per country) while the total economic cost (direct and indirect production losses plus disease control costs) was estimated at 44.8 million euros (3.7 million euros per country). An investment of 14.7 million euros to control CBPP would prevent a loss of 30 million euros. The financial return on investment in CBPP control is positive, with benefit-cost ratios that range from 1.61 (Ghana) to 2.56 (Kenya).

Effect of replacing cotton seed cake with poultry droppings on weight gain of growing cattle at Bambui, Cameroon.

To investigate the effect of replacing cotton seed cake by poultry droppings in the diet of cattle, 30 animals were randomly allocated to three dietary treatments in a 2 x 3 factorial design, sex x treatments. The three treatments consisted of replacing 0%, 25% and 50% (T0, T25, T50 respectively) of the crude protein in 500 g of cotton seed cake with an equivalent amount of protein in poultry droppings. The concentrate offered was 615 g in T0, 652.5 g in T25 and 690 g in T50 per animal per day. After an adaptation period of 14 days, followed by 65 days of the experiment, the intake of concentrate and the average daily weight gains were recorded. There was no significant difference (p > 0.05) in the concentrate intake or average daily weight gain of bulls subjected to the three treatments. However, heifers tended to grow more slowly on T50 compared to T0 (p < 0.05), though the difference in their intake of concentrate was not significant (p > 0.05). Although T50 (47% inclusion in total concentrate), was less palatable, it seemed to have increased forage intake from pasture. These results suggest that poultry droppings can effectively substitute cotton seed cake in the diets of suckler cattle.

Preliminary observations on ticks and tick-borne diseases in the north west province of Cameroon. I. Babesiosis and anaplasmosis.

During a survey on ticks and tick-borne diseases in the North-Western Cameroon at the Bamenda cattle market, the ticks identified were Boophilus annulatus (20%) and B. decoloratus (80%). More than 50% of the ticks were collected during the dry season. Of 524 blood smears 47.3% were positive for Babesia bovis, 31.1% for B. bigemina and 2.2% for Anaplasma marginale. 19.4% were negative.