ABSTRACT
Background: Triazines are environmental active chemicals that have been reported to alter the inflammatory status of the gonads. We tested the anti-inflammatory effect of the triazines (atrazine; ATZ, simazine; SMZ and cyanazine; CYZ) on the testis and compared it with the more classical liver model that has substantial populations of resident macrophages comparable to the testis. Methods: BalB/c mice were treated with 25 mg/kg ATZ, SMZ and CYZ for 30 days and injected with lipopolysaccharide (0.5 mg/kg i.p.) 6 h before sacrifice. Myeloperoxidase activity and nitric oxide level in the testis and liver homogenates were determined by spectrophotometry whereas tumor necrosis factor-alpha and interleukin-6 concentrations were evaluated by immunoassay. Haematoxylin and eosin stained sections of the tissues were observed using a light microscope. Results: Myeloperoxidase activity, nitric oxide, tumor necrosis factor-alpha, and interleukin-6 levels were decreased in the liver and testis of the triazines co-treated animals. SMZ has the most potent inhibitory effect and ATZ the least effect on inflammatory mediators in both tissues. Microscopic evaluation showed loss of inflammatory cells in the inter-tubular areas of the testis and few patchy masses of infiltrating inflammatory cells around the central vein of the liver. Conclusion: Triazines inhibit the levels of inflammatory mediators in the testis and liver of mice. The anti-inflammatory effect of triazines in a lipopolysaccharide-induced inflammation model was established in this study.
ABSTRACT
This study evaluated the beneficial protective effect of cotreatment of curcumin (CUR) and quercetin (QUE) on atrazine (ATZ)-induced testicular toxicity in rats. ATZ challenge diminished luteinizing hormone, follicular stimulating hormone, testosterone and myeloperoxidase enzyme activity, but these effects were attenuated on co-treatment with CUR and QUE. Also, co-treatment of CUR + QUE was better than separate administration of QUE at diminishing malondialdehyde and glutathione and improving tumour necrosis factor-α concentration, germ cell numbers (spermatogonia, spermatocytes and round spermatids) and epididymal sperm quality. Histologically, smaller sized tubules with degenerated epithelia and few germ cells were seen in the seminiferous tubules of the ATZ group whereas CUR + QUE pretreatment improved the histo-morphologic features of the tubules compared to the ATZ group and was also better than separate administration of QUE. We conclude that CUR can improve the protective effects of QUE against ATZ-induced testicular injury by enhancing the levels of reproductive hormones, recovering testicular biochemical parameters and improving the histological features of the testes.
