Phytochemical and GC-MS analysis of Thevetia peruviana fruit methanol extract as an anti-rodenticide potential against balb C rats.

Rodents have remained a menace to humans, hence there is need to evaluate for anti-rodent activity of cheap and environment friendly control methods. This study aimed at evaluating the anti-rodenticidal activity of Thevetia (T.) peruviana fruit methanol extract. T. peruviana fruit was sampled, dried and extracted using methanol/water in the ratio of 3:1 by volume. Phytochemicals; alkaloids, phenols, flavonoids, glycosides, saponins, and tannins were determined qualitatively and quantitatively in the fruit extract. The extract was then characterized using Fourier Transform Infrared (FTIR) and Gas Chromatography Mass Spectrophotometer (GC-MS). Anti-rodent activity of the extracts was determined on a total of 25 mice with body weights of 20-25 g. The animals 8-12 weeks' old were grouped into 5 cages (5 animals per cage), marked and allowed to acclimatize with laboratory conditions of 25 °C, warm or less humid for 5 days with enough water and food. Extract dose (between 0.3 and 0.5 g of extract/kg body weight) was then administered in a single dose by gavage using intubation canula for 7 days and the animals observed for any toxicity and mortality. The data was subjected to probit analysis and ANOVA. Phytochemical screening showed that the extracts contained glycosides, phenols, saponins, alkaloids, triterpenoids, and flavonoids in different abundance. T. peruviana fruit contained 125.13 ± 1.04 mg/g in GAE phenolic content, 85.70 mg/g in RE of dry weight of flavonoids, 10.50 ± 0.01 mg/g in TAE of Tannins, 16.50± 0.21 mg/g alkaloid content, and 8.28± 0.11 mg/g saponin content. The FTIR spectrophotometer depicted O - H, CH2, C[bond, double bond]O, C-O-C functional groups in wave numbers of 3335, 2932, 1599, and 1001 cm-1 respectively. The T. peruviana fruit methanol extracts depicted high acute toxicity with an average of 300 mg/kg upon oral administration in Balb C mice species. The fruit extract from T. peruviana revealed presence of alkaloids, phenols, glycosides, saponins, tannins. These participated synergistically in killing the rats and the postmortem examination report indicated that the tested extract induced a number of physical changes in the mice and therefore the T. peruviana's fruit extract can be utilized as a natural alternative anti-rodent in agriculture production before and after harvesting.

A Cross-sectional Study of Self-Perceived Educational Needs of Emergency Nurses in Two Tertiary Hospitals in Nairobi, Kenya.

BACKGROUND: Many low- and middle-income countries lack resources for well-functioning emergency care systems. Emergency nurses interact with injured and critically ill patients as the first contact in many health care settings. However, insufficient training limits nurses from providing ideal emergency care. The purpose of this research was to highlight educational needs specific to nurses working in 2 emergency departments in Nairobi, Kenya. METHODS: A descriptive cross-sectional study involving emergency units of 2 of the largest referral and teaching hospitals (Aga Khan University Hospital, Nairobi, and Kenyatta National Hospital) in Nairobi, Kenya, was conducted. Data were collected by using an adapted structured, self-administered questionnaire. The data were analyzed using descriptive statistics. The skills and competencies of the participants were assessed. In addition, the educational gaps and needs of the participants around emergency care such as trauma, cardiovascular diseases, and respiratory and neurological illnesses were described. Results were presented in frequencies and percentages. RESULTS: The questionnaire response rate was 63.6% (n = 84). Most of the respondents held associate degrees in nursing (72.6%), whereas 19% had a bachelor's degree in nursing. Most respondents (84.5%) perceived themselves as being highly competent in basic skills such as performing cardiopulmonary resuscitation and assessment of body systems. Less than half of the respondents (48.8%) perceived themselves as being highly competent in intermediate skills such as assisting with endotracheal intubation. In advanced competencies, such as analyzing electrocardiograms and administering thrombolytic medications, only 16.7% perceived themselves as highly competent. CONCLUSION: The results of this study suggest there is a knowledge gap and educational needs among emergency nurses in Nairobi, Kenya. It identified injuries/trauma; cardiovascular, respiratory, and neurological disease; and other emergencies as topics of focus areas with a high need. To address these knowledge and skills needs, a future specialty training in emergency nursing is recommended and this could be achieved through continuing professional development and short courses or postgraduate-level training.

Patterns and outcomes of paediatric trauma at a tertiary teaching hospital in Kenya.

INTRODUCTION: Trauma continues to be a major cause of morbidity and mortality especially in the paediatric population of low- and middle-income countries such as Kenya. The aim of this study was to establish the profile and outcomes of admitted paediatric trauma cases at the Aga Khan University Hospital, Nairobi. METHODS: This retrospective, descriptive study involved a 12-month chart review (January 2016-December 2016). A total of 218 records were identified of which 144 were reviewed. RESULTS: Most injuries were amongst boys (65.3%) and the very young (mean age 6), occurred in private residences (42.4% homes, 25.7% residential institutions), were typically caused by falls (56.3%) or penetrating trauma (13.2%), mostly resulted in extremity fractures (45.8% closed, 4.9% open) and burn or head injuries (in infants and small children), and got very little or no pre-hospital care (51.4% no care). Additionally, children with burns, brain injuries, or poly-trauma had the longest hospital stays and highest rates of mortality. A more detailed description of the patterns and outcomes seen are included in the study. DISCUSSION: Paediatric injuries remain a major public health problem and contribute a substantial proportion of all paediatric surgical admissions at the Aga Khan University Hospital in Nairobi. Based on the patterns and outcomes seen in this study, we therefore recommend for Nairobi (and possibly Kenya) to establish greater supervision and safety measures for children; targeting safety interventions at all children but particularly at boys, the very young, at home and in residential buildings; building pre-hospital emergency care that can accommodate children; and equipping paediatric trauma hospitals to especially handle bony fractures, burns, head injuries, and poly-traumas. A bespoke trauma registry would benefit the hospital, and likely the country as a whole.