1.
Methods Mol Biol
; 2625: 141-148, 2023.
Article
in English
| MEDLINE
| ID: mdl-36653640
ABSTRACT
An optimized Bligh and Dyer protocol and subsequent derivatization is described in this chapter for the extraction of free cholesterol and cholesterol esters from tissue samples. Quantification analysis of lipid species is then described utilizing gas chromatography-mass spectrometry, the ideal method for analysis of volatile organic compounds and extraction of sterols.