ABSTRACT
Acanthamoeba is the causative agent of granulomatous amebic encephalitis, a rare and usually fatal disease. We report a child with acute lymphoblastic leukemia who developed brain abscesses caused by Acanthamoeba during induction therapy. Multimodal antimicrobial chemotherapy and hyperbaric oxygen therapy resulted in complete resolution of symptoms and of pathology as seen by magnetic resonance imaging.
Subject(s)
Acanthamoeba/isolation & purification , Amebiasis/diagnosis , Antiprotozoal Agents/therapeutic use , Central Nervous System Protozoal Infections/diagnosis , Oxygen/therapeutic use , Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications , Acanthamoeba/genetics , Amebiasis/parasitology , Brain/diagnostic imaging , Brain Abscess/diagnosis , Brain Abscess/parasitology , Central Nervous System Protozoal Infections/parasitology , Child, Preschool , DNA, Protozoan/chemistry , DNA, Protozoan/genetics , Encephalitis/diagnosis , Encephalitis/parasitology , Humans , Magnetic Resonance Imaging , Male , Molecular Sequence Data , Radiography , Sequence Analysis, DNA , Treatment OutcomeABSTRACT
BACKGROUND: Childhood immune thrombocytopenia (ITP) is a bleeding disorder characterized by decreased platelet counts. Assessment of the individual bleeding risk during the course of the disease would allow more accurately guiding treatment-related decisions in these patients. PATIENTS AND METHODS: We conducted a pilot study and prospectively evaluated platelet counts and bleeding signs using an established bleeding (Buchanan) score in 30 patients with newly diagnosed ITP at 3 different time points (at diagnosis [TP1], on day 2-3 [TP2], and on day 5-8 [TP3]) during the first week after diagnosis. 15 patients received immune modulatory therapy. RESULTS: Median platelet counts at the 3 different time points were 13, 19, 32×10 (9)/L (untreated patients) and 2, 7, 37×10 (9)/L (treated patients). Corresponding median cumulative bleeding scores were 5, 2, 0 (untreated patients) and 7, 6, 2 (treated patients). Cumulative median bleeding scores and platelet counts were inversely correlated in treated and untreated patients at all 3 time points. Cumulative median bleeding scores significantly decreased in both groups. CONCLUSIONS: Bleeding signs in children with newly diagnosed ITP rapidly improve within one week after diagnosis. Serial grading of bleeding severity seems to be useful to comprehensively assess and monitor the individual bleeding risk in these patients, but has to be evaluated and validated in a larger cohort.
Subject(s)
Hemorrhage/diagnosis , Hemorrhage/immunology , Purpura, Thrombocytopenic, Idiopathic/diagnosis , Purpura, Thrombocytopenic, Idiopathic/immunology , Adrenal Cortex Hormones/therapeutic use , Child , Child, Preschool , Combined Modality Therapy , Female , Hemorrhage/therapy , Humans , Immunization, Passive , Male , Pilot Projects , Platelet Count , Prospective Studies , Purpura, Thrombocytopenic, Idiopathic/therapyABSTRACT
A 14-year-old girl was diagnosed with alveolar soft part sarcoma (ASPS) of the thigh and lung metastases. She underwent tumor resection and pulmonary metastasectomy followed by hyperfractionated local radiotherapy (44.8 Gy). A mesh graft transplant was used to cover the postoperative skin defect on the thigh. Since ASPS do not respond to conventional chemotherapy antiangiogenic treatment with peginterferon alfa-2b and thalidomide was started. Immunohistochemical analysis of tumor tissue showed expression of vascular endothelial growth factor receptors (VEGFR) 1, 2, 3, and platelet derived growth factor receptor (PDGFR)-alpha and -beta. Hence, additional treatment with multitargeted receptor tyrosine kinase inhibitor sunitinib (Sutent) was started on a compassionate use basis. 2 weeks later the patient presented with necrosis of the skin transplant requiring necrectomy and skin grafting. This case illustrates that drugs inhibiting vascular endothelial growth factor receptors have to be used very cautiously in cancer patients with severe pre-existing skin damage.