ABSTRACT
Using the direct leukocyte migration inhibition assay, the cell-mediated immune response was followed in together 47 patients suffering from herpes zoster. Of these, 19 persons were treated with partially purified and concentrated lysed leukocyte ultrafiltrate. Enhancement and/or earlier production of the leukocyte migration inhibition factor--in the presence of varicella-zoster virus antigen--was observed by leukocytes from patients given the ultrafiltrate on days 2-3 since the onset of the vesicular stage of the disease. Highly significant (alpha = 1%, P less than 0.01) differences in the migration inhibition values were observed between non-treated patients and patients given one dose of the ultrafiltrate during the days 3-12 after appearance of the first herpes zoster vesicles; on days 13-30 these values were not significant.
Subject(s)
Biological Factors/therapeutic use , Herpes Zoster/therapy , Leukocyte Migration-Inhibitory Factors/biosynthesis , Leukocytes/immunology , Lymphokines/biosynthesis , Adolescent , Adult , Aged , Biological Factors/isolation & purification , Child , Herpes Zoster/immunology , Herpesvirus 3, Human/immunology , Humans , Immunity, Cellular/immunology , Middle Aged , UltrafiltrationSubject(s)
Meningitis, Listeria/epidemiology , Adult , Aged , Czechoslovakia , Humans , Male , Meningitis, Listeria/diagnosis , Middle AgedABSTRACT
Gel filtration through Sephadex G-200 or Ultrogel AcA 34 minicolumns was applied to isolate IgM antibodies from sera of patients with varicella and herpes zoster (HZ). Serum samples were stained with Sudan III and Evan's Blue in order to monitor the separation process visually when running several columns at the same time. The presence of specific IgM antibodies was demonstrated by enzyme linked immunosorbent assay (ELISA). Rapid separation of stained sera by diffusion chromatography on minicolumns with successive determination of specific IgM antibodies in ELISA proved suitable for serological diagnosis of varicella, whereas the significance of IgM-specific antibody response in HZ seems uncertain.
Subject(s)
Antibodies, Viral/isolation & purification , Chickenpox/diagnosis , Herpes Zoster/diagnosis , Herpesvirus 3, Human/immunology , Immunoglobulin M/isolation & purification , Acute Disease , Antibodies, Viral/analysis , Cell Line , Chromatography, Gel , Enzyme-Linked Immunosorbent Assay , Humans , Immunoglobulin G/analysis , Immunoglobulin G/isolation & purification , Immunoglobulin M/analysis , Predictive Value of TestsABSTRACT
A study of an outbreak of tick-borne encephalitis (TBE) in a natural focus of this infection at ZAvada village (Slovakia) led to the conclusion that apparently the epidemic was caused by consumption of fresh sheep cheese and infection of persons occurred by the alimentary route. To our knowledge, this is the first record of this source of infection in man.
