ABSTRACT
An ectopic left kidney together with an aberrant vascular structure were identified during a routine sterilization procedure in an eighteen-month-old female Akita Inu. Subsequently, ultrasound and computed tomography were performed to evaluate the clinical relevance. Based on these examinations' findings, a simple unilateral left-sided ectopic kidney with an abnormal course of the left renal artery as well as an earlier division of the right-sided renal artery were diagnosed. An ectopic kidney is clinically relevant differential diagnosis of secondary renal displacement or abdominal mass.
Subject(s)
Kidney , Renal Artery , Female , Dogs , Animals , Renal Artery/diagnostic imaging , Kidney/diagnostic imaging , Clinical Relevance , Sterilization, Reproductive/veterinary , Tomography, X-Ray ComputedABSTRACT
Molecular profiling of a tumor allows the opportunity to design specific therapies which are able to interact only with cancer cells characterized by the accumulation of several genomic aberrations. This study investigates the usefulness of next-generation sequencing (NGS) and mutation-specific analysis methods for the detection of target genes for current therapies in non-small-cell lung cancer (NSCLC), metastatic colorectal cancer (mCRC), and melanoma patients. We focused our attention on EGFR, BRAF, KRAS, and BRAF genes for NSCLC, melanoma, and mCRC samples, respectively. Our study demonstrated that in about 2% of analyzed cases, the two techniques did not show the same or overlapping results. Two patients affected by mCRC resulted in wild-type (WT) for BRAF and two cases with NSCLC were WT for EGFR according to PGM analysis. In contrast, these samples were mutated for the evaluated genes using the therascreen test on Rotor-Gene Q. In conclusion, our experience suggests that it would be appropriate to confirm the WT status of the genes of interest with a more sensitive analysis method to avoid the presence of a small neoplastic clone and drive the clinician to correct patient monitoring.
