ABSTRACT
The dynamics of microbial biofilm formation by standard strain and by clinical strains of uropathogenic coliform bacteria was investigated in vitro and the effect of sublethal concentrations of the polymer compound polyazolidinammonium modified with iodine hydrate ions on the initial stages of biofilm formation was assessed. Treatment of immunological plate wells with the polymeric compound prevented film formation, especially in case of clinical E. coli strain carrying FimH virulence gene.
Subject(s)
Anti-Bacterial Agents/pharmacology , Azoles/pharmacology , Biofilms/drug effects , Polymers/pharmacology , Quaternary Ammonium Compounds/pharmacology , Uropathogenic Escherichia coli/drug effects , Anti-Bacterial Agents/chemical synthesis , Azoles/chemical synthesis , Bacterial Adhesion/drug effects , Biofilms/growth & development , Gentian Violet , Iodine/chemistry , Microbial Sensitivity Tests , Polymers/chemical synthesis , Quaternary Ammonium Compounds/chemical synthesis , Uropathogenic Escherichia coli/pathogenicity , Uropathogenic Escherichia coli/physiology , VirulenceABSTRACT
AIM: Study of antimicrobial activity of a polymer compound--polyazolidinammonium, modified with hydrate-ions of iodine. MATERIALS AND METHODS: Antimicrobial activity of polyazolidinammonium, modified with hydrate-ions of iodine, against reference strains and clinical isolates of Gram positive and negative bacteria, microscopical fungi, as well as RNA viruses was studied. RESULTS: High antibacterial activity of the studied compound was established, especially against Gram positive bacteria. A higher concentration of the preparation (125-250 µg/ml) was characterized by anti-fungal effect. A high sensitivity to polymer of swine transmissible gastroenteritis virus was noted. CONCLUSION: The polymer compound, based on the results of the studies, is a perspective antiseptic and etiotropic means for control of infectious disease causative agents.
