ABSTRACT
In previous studies a series of Mabs against boar capacitated sperm have been produced. One of these Mabs--4B12--was found to recognize a surface membrane-associated protein located in the acrosome portion of the spermatozoa that became accessible to antibody after capacitation. In biological experiments it was shown that Mab 4B12 significantly inhibited boar sperm-porcine ZP binding. In attempts to investigate the mechanisms by which Mab 4B12 affected sperm-ZP binding, the role of the cognate protein on some functional parameters such as sperm motility and ability of the capacitated spermatozoa to undergo AR was studied. Experimental models of premature AR and AR physiologically induced with ZP were applied to study the effect of Mab 4B12 on boar sperm AR using PSA staining to estimate the acrosome-reacted state of spermatozoa. Sperm motility characteristics were determined by the time-exposure photokinesigraphic method. The results obtained in the present study, together with previously established inhibition of sperm-ZP binding by Mab 4B12, documented the participation of the 4B12 protein in primary sperm-ZP binding. The protein is not connected with sperm motility and secondary sperm-ZP binding.
Subject(s)
Antibodies, Monoclonal/metabolism , Membrane Proteins/metabolism , Sperm Capacitation/physiology , Spermatozoa/physiology , Acrosome Reaction , Animals , Male , Membrane Proteins/immunology , Protein Binding , Sperm Motility , Spermatozoa/cytology , Swine , Zona Pellucida/metabolismABSTRACT
A longitudinal study of 251 children with primary hypertension (PH), aged 6-15 from Sofia, Bulgaria, was carried out over a 10-year period (1972-1983). The dynamics of blood pressure (BP), target organ changes, and risk-factors influence were followed up. In the tenth year, 44.2% of the persons remained hypertensive, 29.9% were found to be with borderline hypertension, and 25.9% with normal BP. Functional and structural changes in the heart were discovered in a considerable number of the persons by ECG, x-ray, and echocardiography examinations. Furthermore, angioretinopathy I and II gr. in 24.0%, hyperlipoproteinemia (IIA, IIB, and IV type) in 45.3% and low levels HDL-chol. (less than or equal to 0.905 mmol/l) in 34.5% were present at the end of the study in those young persons who had PH in childhood. A control group of 145 children with normal BP in 1972-1973 has been followed up over the same period.
Subject(s)
Hypertension/genetics , Obesity/complications , Adolescent , Adult , Blood Pressure , Bulgaria , Child , Electrocardiography , Female , Follow-Up Studies , Fundus Oculi , Humans , Lipids/blood , Male , Risk FactorsABSTRACT
Sustained hypertension (H) was found in 6% of 21,500 school children aged 6 to 15 years studied in 1972-73 and again in 1982-83. The primary character of H was proved in 85%. Hemodynamic studies revealed a hyperkinetic type of circulation and elevated total peripheral resistance. Studies on lipid and carbohydrate metabolism showed a high rate of hyperlipoproteinemia with low levels of HDL-cholesterol and impaired glucose tolerance combined with high basal plasma triglycerides and plasma insulin before and after stimulation by glucose. In a follow-up study 10 years after the first one we established persistent H in 43% of the initially hypertensive children.
