ABSTRACT
We have examined for the first time the relationship between the expression of PAPP-A metalloproteinase and insulin-like growth factors (IGF-I, IGF-II, VEGF) and transcription factors (NF-kappaB, HIF-1) playing an important role in pathogenesis of cancer. We also demonstrated a positive association between the level of PAPP-A metalloproteinase and the level of growth (VEGF and IGF-I) and transcription factors (NF-kappaB p50, NF-kappaB p65, HIF-1alpha). The current findings suggest an important role of PAPP-A in regulation of bioavailability of IGF-I, VEGF, activated forms of NF-kappaB, and alpha-subunits of HIF-1 in endometrial tumors.
Subject(s)
Endometrial Neoplasms/genetics , Gene Expression Regulation, Neoplastic , Pregnancy-Associated Plasma Protein-A/metabolism , Endometrial Neoplasms/pathology , Female , Humans , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Insulin-Like Growth Factor I/genetics , Insulin-Like Growth Factor I/metabolism , Insulin-Like Growth Factor II/genetics , Insulin-Like Growth Factor II/metabolism , Middle Aged , NF-kappa B/genetics , NF-kappa B/metabolism , Neoplasm Staging , Pregnancy-Associated Plasma Protein-A/genetics , Transcription Factors/biosynthesis , Vascular Endothelial Growth Factor A/genetics , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The aim of the study was investigation of morphological changes in the urinary bladder and ureteral wall of dogs in response to contact electroimpulse impact (CEII); to develop recommendations for utilization of contact electroimpulse lithotripsy in humans. Effects of single-impulse CEII on the bladder and ureteral mucosa produced by an electroimpulse lithotripter were studied in 23 mature mongrel dogs. The morphological material was studied immediately after the impact, 1, 6, 14 days and 1, 3, 6 and 12 months after it. The 0.1-0.7 J electroimpulse impact resulted in fragmentary epithelial necrosis and aceptic inflammation within a muscular layer. An enhanced to 0.8 J impact caused local damage to all layers of the wall including adventitium while a 0.9-1 J impact caused ureteral perforation in 3 cases. Duration of morphological rehabilitation depended on the impact power and was optimal for 0.1-0.5 J impulses with complete mucosal repair to day 14 and complete morphological rehabilitation to month 6 after the procedure. In power 0.6-1 J mucosal recovery increased to 1 month while complete formation of the connective tissue finished after 1 year. None cases of development of ureteral strictures after CEII was observed in follow-up for 1 year.
Subject(s)
Electric Stimulation Therapy , Urethra/pathology , Urethra/physiopathology , Urinary Bladder/pathology , Urinary Bladder/physiopathology , Animals , Dogs , Female , MaleABSTRACT
The recombinant avian adenovirus CELO-gpRb expressing glycoprotein G of rabies virus (strain TS-80, ARRIW&M, Pokrov, Russia) was used for mice vaccination against rabies. Double intramuscular immunization by recombinant CELO-gpRb adenovirus in a dose 10(9) pfu per mouse caused the induction of virus neutralizing antibodies (VNA) synthesis in 78% of mice, while twice repeated intradermal injections of the recombinant adenovirus failed to induce the VNA production. The protection level in groups of vaccinated mice after intracerebral injection of CVS rabies virus in a dose of 100 MLD50 was equal to 45% at single intramuscular immunization and to 91% after twice repeated intramuscular immunization. The recombinant adenoviral vaccine against rabies, based on CELO viral genome, has a good perspective for domestic and wild animal vaccination, not only due to rather high protection level, but also because the production of adenoviral CELO vaccine in chicken embryos is of high technology and inexpensive.
Subject(s)
Antigens, Viral/immunology , Aviadenovirus/metabolism , Glycoproteins/immunology , Immunization Schedule , Rabies Vaccines/immunology , Rabies virus/immunology , Rabies/immunology , Rabies/prevention & control , Reassortant Viruses/metabolism , Viral Envelope Proteins/immunology , Animals , Antibodies, Viral/blood , Antigens, Viral/biosynthesis , Glycoproteins/biosynthesis , Injections, Intramuscular , Mice , Mice, Inbred BALB C , Neutralization Tests , Rabies/blood , Rabies Vaccines/administration & dosage , Vaccines, Synthetic/administration & dosage , Vaccines, Synthetic/immunology , Viral Envelope Proteins/biosynthesisSubject(s)
Antibodies, Viral/blood , Rabies virus/immunology , Rabies/blood , Rabies/prevention & control , Vaccination , Animals , Antibodies, Viral/immunology , Cell Line , Enzyme-Linked Immunosorbent Assay/methods , Enzyme-Linked Immunosorbent Assay/standards , Humans , Predictive Value of Tests , Rabies/immunologyABSTRACT
Methods for evaluating the potency of inactivated rabies vaccines are reviewed. Shortcomings of the traditional NIH method and advantages of modern rapid immunological in vitro methods (antibody binding test, radial immunodiffusion test, enzyme linked immunoadsorbent assay) for estimation of antigenic activity of vaccines are discussed.
