ABSTRACT
The ultrastructure of the leaf epidermis and the localization and content of silicon in epidermal cells of the psammophyte Alyssum desertorum Stapf (Brassicaceae) were investigated using electron microscopy and laser confocal microscopy. The study aimed to examine the effect of flooding on the epidermal structure of the leaves, study the presence, localization, and content of silicon in the epidermal cells and determine whether changes in soil moisture affect the silicon content in the leaf epidermis. Silicon inclusions were observed in trichomes and typical epidermal cells on both leaf surfaces, with varying content depending on the cell type, leaf surface, and conditions growth. Soil flooding was found to influence the leaf epidermis ultrastructure and the silicon content in the epidermal cells of A. desertorum. Trichomes and pavement cells were identified as the main accumulators of silicon inclusions and wax-like structures. These findings suggest that changes in leaf microstructure and silicon content contribute to the normal functioning of psammophyte plants and can be considered signs of plant phenotypic plasticity and adaptive markers depending on water supply conditions.
ABSTRACT
The microstructure of leaf epidermis, the localization, and content of syringyl (S) and guajacyl (G) monolignol in epidermal cells of Phragmites australis grown in water and moderate drought soil on the bank of the Venetian strait of the Dnipro River in Kiev (Ukraine) were studied with the electron microscopic method and laser confocal microscopy. The trichomes, guard cells of stomata, epidermal cells of vaulted zones over veins of adaxial and abaxial surface contained syringyl and guajacyl, the content of which depended on plant ecotype. We established that moisture soil influenced the leaf epidermis ultrastructure, on the change of monolignols content and S/G ratio in epidermis leaf of reed. The investigations show that trichomes and the cells of the vaulted zone in the leaf epidermis were the main accumulator of monolignols. The obtained results suggest that change of the microstructure of leaves, content of the monolignols, and S/G ratio in leaves contribute to the preservation of optimal water status of plants and can be considered as the signs of plant phenotypic plasticity and adaptive marker depending on the conditions of water supply.
Subject(s)
Ecotype , Poaceae , Epidermal Cells , Epidermis , Plant LeavesABSTRACT
The goal of our work was a role of phosphorylase (EC. 2.4.1.1) in starch accumulation in plastids of storage parenchyma cells in potato minitubers forming under clinorotation. An increased enzyme activity under the influence of simulated microgravity has been revealed by using the biochemical and electron cytochemical methods. The obtained results suggest the correlation between an increase in phosphorylase activity and acceleration growth rate and senescence of plant storage organs in microgravity.
