ABSTRACT
It is known that inflammatory cytokines, which level is significantly increased in the pathogenesis of multiple sclerosis (MS), as well as interferon-beta, which is used to treat autoimmune diseases, can inhibit cytochrome P450-dependent processes of detoxification and biotransformation. The uncontrolled decrease of the activity of these processes may have a negative affect on the state of patients, so it is urgent to study the functional state of the cytochrome P450 system and to develop effective means for its regulation in these conditions. The effect of vitamin D3 and efficiency of its composition with vitamins B1, B2, B6, PP, E, alpha-lipoic, alpha-linolenoic acid and mineral substances (Mg, Zn, Se) in prevention of a functional state changes of cytochrome P450- and b5-dependent systems of the rat brain and liver endoplasmic reticulum at EAE are investigated. It has been shown that the essential decrease of the level of these cytochromes is observed both in the brain and liver. In addition the level of activity of NADH- and NADPH-oxidoreductases, which are part of microsomal electron transport chain components and coupled with monooxigenases, was reduced. These changes confirm the disturbances of a redox state and functional activity of detoxication and biotransformation systems in the studied animal tissues. Supplement of vitamin D3 as well as the composition of biologically active substances, which we developed earlier, effectively eliminated the decrease of the level of cytochromes and activities of NADH-oxidoreductase in immunised rat tissues. Normalization of these disturbances can be explained by antioxidant and membrane-stabilizing properties of applied substances, and also by the ability to reduce the activity of inflammatory reactions by regulation of the level of inflammatory cytokines in rat organism at EAE. Thus the studied vitamin-mineral composition appeared to be more effective to normalize the found disturbances and it can be useful for prevention of exacerbations and for improvement of a status of patients with multiple sclerosis and other diseases, which are accompanied with hyperactivation of immune system.
Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Encephalomyelitis, Autoimmune, Experimental/drug therapy , Magnesium/administration & dosage , Minerals/administration & dosage , Selenium/administration & dosage , Vitamins/administration & dosage , Zinc/administration & dosage , Animals , Brain/drug effects , Brain/enzymology , Brain/immunology , Carrier Proteins/agonists , Carrier Proteins/metabolism , Encephalomyelitis, Autoimmune, Experimental/chemically induced , Encephalomyelitis, Autoimmune, Experimental/enzymology , Encephalomyelitis, Autoimmune, Experimental/pathology , Freund's Adjuvant , Heme-Binding Proteins , Hemeproteins/agonists , Hemeproteins/metabolism , Liver/drug effects , Liver/enzymology , Liver/immunology , Male , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Microsomes, Liver/immunology , Myelin Proteins , Rats , Rats, WistarABSTRACT
The effects of hyperthyreosis on oxidative stress, state of glial intermediate filaments and memory were investigated. We observed a significant increase in lipid peroxidation products into both hippocampus and cortex and memory worsening. The changes of GFAP polypeptides was observed in hippocampus and cortex. In group of rats with hyperthyreosis, the content of GFAP in both soluble and filamentous fractions was increased in hippocampus. This data shows, that glial cytoskeleton is reconstructed under thyroid hormone effects.
Subject(s)
Brain/physiopathology , Cognition/physiology , Glial Fibrillary Acidic Protein/metabolism , Hyperthyroidism/physiopathology , Memory/physiology , Neurofilament Proteins/metabolism , Animals , Brain/metabolism , Brain/ultrastructure , Cytoskeleton/metabolism , Cytoskeleton/ultrastructure , Disease Models, Animal , Female , Hyperthyroidism/metabolism , Hyperthyroidism/pathology , Immunoblotting , Male , Oxidative Stress , Rats , Rats, WistarABSTRACT
The expression and polypeptide composition of Fc-gamma-receptors of enterocytes from Bos primigenius taurus L. intestine at 3-, 5-, 7-, 9- month of fetal development was investigated. The results of immunobloting show similar composition of Fc-gamma-receptors extracted from apical and basolateral membranes. The proteins that bind IgG after PAAG electrophoresis and transferring on nitrocellulose were observed as 120, 87, 72 and 43 kDa polypeptide line. The changes of each polypeptide contents were related to the changes of total content of Fc-gamma-receptor proteins. The rise in concentration of Fc-gamma-receptors on apical membrane was observed from 3-rd to 7-th month of fetal development. Maximal concentration of these proteins was detected on enterocytes at 7-th month of fetal development. Fc-gamma-receptors content on basolateral side was higher than on apical side. The presence of Fc-gamma-receptors on enterocyte's membrane indicates on active recycling of these receptors on plasma membrane and reflects early development of immune system in Bos primigenius taurus L. fetus.
Subject(s)
Enterocytes/metabolism , Jejunum , Peptides/chemistry , Receptors, IgG/biosynthesis , Animals , Cattle , Cell Membrane/metabolism , Cells, Cultured , Electrophoresis, Polyacrylamide Gel , Enterocytes/immunology , Fetal Development/immunology , Gestational Age , Immunoglobulin G/immunology , Immunoglobulin G/metabolism , Jejunum/cytology , Jejunum/embryology , Jejunum/immunology , Jejunum/metabolism , Protein Binding , Protein Conformation , Receptors, IgG/chemistryABSTRACT
The effect of streptozotocin-induced diabetes on glial intermediate filament and behavioral reactions of rats was investigated. The water Morris test shows cognitive deficit in diabetic rats. The results of an immunoblot show both an increase in GFAP degradation and an increase in common GFAP amount in the brain of diabetic rats. The observations presented here suggest that diabetes induces the changes in activity of GFAP-positive glial cells. Such glial cells may be considered a key element in plasticity of nervous system. These results suggest that the impairment of learning and memory is accompanied by glial cytoskeletal reconstruction.
Subject(s)
Behavior, Animal/physiology , Diabetes Mellitus, Experimental/physiopathology , Glial Fibrillary Acidic Protein/biosynthesis , Learning/physiology , Animals , Cerebellum/metabolism , Cerebral Cortex/metabolism , Diabetes Mellitus, Experimental/metabolism , Hippocampus/metabolism , Immunoblotting , Male , Rats , Rats, WistarABSTRACT
The short characteristic of cell adhesion molecules and the main components of cytoskeleton are submitted at the present review. Structural and functional peculiarities of these proteins are presented. On the basis of different sources an attempt to clarify the possible mechanism of the interaction of adhesion proteins cytoskeleton structure has been done. It is suggested that information about adhesion contacts is transmitted from the adhesion proteins to cytoskeleton structures that induces the transformation of the letter ones and promotes the cellular metabolic response.
