Kinetics of PCB Microbial Dechlorination Explained by Freely Dissolved Concentration in Sediment Microcosms.

While microbial dechlorination of polychlorinated biphenyls (PCBs) has been observed in sediments over the last 3 decades, translation to the field has been difficult due to a lack of a clear understanding of the kinetic limitations. To address this issue, the present study used passive dosing/sampling to accurately measure the biological rate of dechlorination of 2,3,4,5-tetrachlorobiphenyl (PCB 61) to 2,3,5-trichlorobiphenyl (PCB 23) by an organohalide-respiring bacterium, Dehalobium chlorocoercia (DF-1). The biological rates were measured over an environmentally relevant concentration range of 1-50 ng/L of freely dissolved concentrations with and without the presence of sediment in bench-scale microcosm studies. The rate of dechlorination was found to be linearly dependent on the freely dissolved concentration of PCB 61 both in sediment and in sediment-free microcosms. The observed rate of dechlorination in sediment microcosms could be predicted within a factor of 2 based on the kinetics measured in sediment-free microcosms. A threshold for dechlorination was not observed down to an aqueous concentration of about 1 ng/L PCB 61. We demonstrate that with the combination of an accurate measurement of the aqueous-phase dechlorination kinetics and an understanding of the site-specific partitioning characteristics, it is possible to predict PCB microbial dechlorination in sediments.

Four decades since the ban, old urban wastewater treatment plant remains a dominant source of PCBs to the environment.

Despite the ban on new manufacture and commercial use of PCBs, municipal sewer systems continue to serve as ongoing secondary sources for contamination in receiving water bodies. Ongoing PCB sources have made it difficult to achieve desired recovery after implementation of sediment cleanup efforts. We report on a 16-month surveillance to determine the inputs, fate, and export of PCBs within a municipal waste collection/treatment system by strategic sampling of the freely-dissolved and biosolids-associated PCBs. The total PCBs entering the treatment plant was found to be 170 g/day of which 100 g/day exited the plant associated with the biosolids and 5.2 g/day was discharged in the form of freely-dissolved PCBs in the effluent. A net loss of 68 g/day was calculated for the plant, attributable to volatilization and biodegradation. Freely dissolved PCBs in the treated effluent was an order of magnitude higher than the water quality criteria for the protection of human health through fish consumption and found to be a major contributor to the dissolved concentration in the receiving river. Predicted bioaccumulation in fish from dissolved PCBs in the effluent exceeded the threshold for human consumption. The biosolids, currently land-applied as fertilizer, contained an average PCB concentration of 760 µg/kg. The sludge produced in this treatment plant is processed in large anaerobic digesters and changes to the homolog distribution point to some microbial dechlorination. Application of biosolids to clean agricultural soil resulted in a 6-fold increase in PCB levels in the earthworm E. fetida which could be eliminated by the amendment of 1% by weight of activated carbon.