[In-vitro effect of X-irradation on respiration and glycolysis of Ehrlich-ascitic carcinoma cells of the mouse--an experimental comparison with the mouse tumour-tetanus assay (author's transl)]. / In-vitro-Wirkung von Röntgenstrahlen auf Atmung und Gärung von Ehrlich-Aszites-Karzinomzellen der Maus--ein experimenteller Vergleich mit dem Maus-Tumor-Tetanus-Test.

Depending on the dose of X-rays, in-vitro irradiation of Ehrlich-ascitic carcinoma cells of the mouse affected both respiration and glycolysis. 15,000 R irradiation suppressed the aerobic and anaerobic energy metabolism rather strongly followed by a reduction of the "take" and growth of the subcutaneously injected tumour cells, as opposed to the growth behaviour of non-irradiated cells. In analogy, tetanus mortality rates were reduced in the mouse tumour-tetanus assay with 15,000 R irradiated cells. On the other hand, irradiation with 2,000 R of Ehrlich carcinoma cells resulted in unchanged rates of respiration and glycolysis, in spite of the strongly limited growth capacity of the tumour cells. The tumour-tetanus assay of the mouse showed good correlation with subcutaneous tumour growth; no such correlation was found in the tetanus assay and the manometric values of respiration and glycolysis with 2,000 R irradiated tumour cells.--After subcutaneous injection of mixed cell suspensions consisting of 1 x 10(5) viable and 1 x 10(6) 15,000 R irradiated Ehrlich-ascitic carcinoma cells as well as of 3 x 10(2) tetanus spores per single dose, we observed similar rates of tumour growth, or tetanus mortality, respectively, if 1 x 10(5) viable tumour cells alone were administered together with 3 x 10(2) tetanus spores, without addition of irradiated tumour cells.

[Experiments concerning the enhancement of clostridial growth (Clostridium butyricum 1672 A McClung) by means of the anaerobic metabolism of Ehrlich ascites carcinoma cells of the mouse (author's transl)]. / Versuche zur Stimulierung des Klostridienwachstums (Clostridium butyricum 1672 A, McClung) durch den anaeroben Stoffwechsel von Ehrlich-Aszites-Karzinomzellen der Maus

Ehrlich ascites carcinoma cells stimulate the vegetative growth of Clostridium butyricum in a modified Parker medium under N2 atmosphere. The enhancement of clostridial growth depends on the number of tumour cells added. The Ehrlich ascites carcinoma cells keep the ordinary transplantability after anaerobic incubation with the vegetative forms of Clostridia. Further means for the manometric investigations of the tumour clostridium phenomenon are discussed.