ABSTRACT
The biogenic manufacture of nanoparticles utilising endophytic fungus is an eco-friendly, cost-effective, and secure alternative to constructing chemical methods. The prime focus of the study was to fabricate ZnONPs using the biomass filtrate of endophytic Xylaria arbuscula isolated from Blumea axillaris Linn. and to evaluate their biological properties. The characterisation of the biosynthesized ZnO-NPs was done utilising both spectroscopic and microscopic methods. The bioinspired NPs showed a surface plasmon peak at 370 nm; SEM and TEM micrographs illustrated the hexagonal organisation; XRD spectra proved the crystalline phase as hexagonal wurtzite; EDX analysis confirmed the presence of zinc and oxygen atoms; and the zeta potential analysis proved the stability of ZnONPs. In addition, they also demonstrated significant concentration-dependent inhibition of antimicrobial, antioxidant, anti-inflammatory, and antidiabetic potential in comparison with the reference drugs. In vitro cytotoxicity and wound healing potential of ZnONPs were examined in L929 cell lines, illustrating that they accelerated the wound healing process by roughly 95.37 ± 1.12% after a 24-h exposure to ZnONPs. The photocatalytic activity of the ZnONPs was examined by degrading the methylene blue dye under solar irradiation. In conclusion, our outcomes showed that mycosynthesized ZnONPs possessed potent bioactivity and could be an excellent choice for biomedical applications.
Subject(s)
Ascomycota , Asteraceae , Zinc Oxide , Zinc Oxide/pharmacology , Antioxidants , Plant Extracts/pharmacologyABSTRACT
The development of MnO2-graphene (MnO2-GR) composite by microwave irradiation method and its application as an electrode material for the selective determination of serotonin (SE), popularly known as "happy chemical", is reported. Anchoring MnO2 nanoparticles on graphene, yielded MnO2-GR composite with a large surface area, improved electron transport, high conductivity and numerous channels for rapid diffusion of electrolyte ions. The composite was characterized by X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and scanning electron microscopy (SEM) for assessing the actual composition, structure and morphology. The MnO2-GR composite modified glassy carbon electrode (GCE) exhibited an excellent electrochemical activity towards the detection of SE in phosphate buffer saline (PBS) at physiological pH of 7.0. Under optimum conditions, the modified electrode could be applied to the quantification of serotonin by square wave voltammetry over a wide linear range of 0.1 to 800 µM with the lowest detection limit of 10 nM (S/N = 3). The newly fabricated sensor also exhibited attractive features such as good anti-interference ability, high reproducibility and long-term stability.
Subject(s)
Electrochemical Techniques/methods , Graphite/chemistry , Manganese Compounds/chemistry , Oxides/chemistry , Serotonin/chemistry , ElectrodesABSTRACT
Biosurfactants are one among the best alternative for synthetic surfactants that are exploited by many researchers. Several agro wastes help to reduce the cost biosurfactants by being renewable and economical. The present research focuses on the biosurfactant production from Pseudomonas mosselii utilizing Parthenium hysterophorus as a relatively cheap substrate. P. hysterophorus being a hazardous weed, its eradication is quite tedious. So, the utilization of the weed for useful purposes serves as a choice to overcome the problems posed by the weed. In the study, this weed has been successfully utilized as a substrate and the optimized fermentative production of biosurfactant was done. From one-factor-at a-time analysis it was known that the substrate level of 3% incubation time of 96 h, pH 6.0, temperature 35 °C, glucose and yeast extract was found to be the best C and N sources for a high yield. The extracted biosurfactant was partially purified and characterized using FTIR. The biosurfactant produced from the weed could help to render the milestone for distinct biomedical and other applications.
