[Immune defense in hormone-dependent bronchial asthma]. / Immunnaia zashchita pri gormonal'no-zavisimoi forme bronkhial'noi astmy.

The study was undertaken to examine the clinical and immunological features of hormone-dependent bronchial asthma. Long-term corticosteroid therapy was found to have a pronounced immunodepressive effect on both lymphocytic subpopulations and opsonophagocytosis. The sequelae of immunological disorders are lower anti-infective defense, which contributes to a more severe course of the disease generally running in the presence of chronic obstructive bronchitis. The use of immunomodulators, extracorporeal techniques, which favours to reduce the adverse immunosuppressive effect of long-term therapy, is an alternative to corticosteroid dosage increment.

[Ribonuclease stimulation of nonspecific factors of protection in experimental animals]. / Stimuliatsiia ribonukleazami nespetsificheskikh faktorov zashchity v organizme éksperimental'nykh zhivotnykh.

It was shown that a single intraperitoneal administration of Bacillus intermedius RNAse to rats stimulated the activity of lysozyme and blood serum complement. A single intraperitoneal administration of pancreatic RNAse, Bacillus intermedius RNAse and its derivative selectively inactivated by the histidine active centre stimulated the metabolic activation of neutrophils as was shown by their ability to reduce tetrazolium nitroblue to diphormazone. The efficiency of the neutrophil stimulation by the RNAses was comparable with that of the microbial vaccine and did not depend on the catalytic activity of the RNAses.

[The cytomorphological evaluation of the effect of Bacillus intermedius RNAse on the organs of experimental animals]. / Tsitomorfologicheskaia otsenka vliianiia RNKazy Bacillus intermedius na organy éksperimental'nykh zhivotnykh.

Cytomorphological changes in organs of experimental animals after RNAse Bacillus intermedius treatment have been studied. It has been found that RNAse stimulates elements of lympho- and homopies of thymus and spleen and increases stromal reaction and functional activity of liver. The caused effects don't depend on catalytic enzyme activity.

[The histological evaluation of the effect of Bacillus intermedius RNAse on the organs of immunogenesis in laboratory animals]. / Gistologicheskaia otsenka vliianiia RNKazy Bacillus intermedius na organy immunogeneza laboratornykh zhivotnykh.

Histological changes in organs of immunogenesis of experimental animals after RNAse Bacillus intermedius treatment have been studied. The effect of RNAse on organs is revealed in activation of both cell and humoral immunity and factors of nonspecific resistance at early stage of reaction. The inhibition of immunological reactions and factors of nonspecific resistance takes place at later stages of enzyme action. The caused effects don't depend on catalytic enzyme activity.

[Cytomorphologic assessment of the effect of Bacillus intermedius RNase on the organs of experimental animals]. / Tsitomorfologicheskaia otsenka vliianiia RNKazy Bacillus intermedius na organy éksperimental'nykh zhivotnykh.

Cytomorphological changes in the organs of laboratory animals after their treatment with RNAse from Bacillus intermedius were investigated. It was shown that the effect of RNAse on the thymus and spleen was evident from stimulation of the elements of the lympho and hemopoiesis while its effect on the liver manifested itself in increased stromal response of the liver and higher functional activity of the organ. The observed effects did not depend on catalytic activity of the enzyme.

[Influence of Bacillus intermedius RNAase on thymus cell composition and T-lymphocyte differentiation in an experiment]. / Vliianie rnkazy Bacillus intermedius na kletochnyi sostav timusa i differentsirovku T-limfotsitov v éksperimente.

It was shown that exposure of mice AKR and (CBA X C57B1/6)F1 to Bacillus intermedius RNAase and its derivative selectively inactivated by the active centre histidine stimulated T-lymphocyte maturation. With using bone marrow and spleen lymphocytes of congenitally thymus-free mice it was revealed that this effect of RNAase was defined by induction of Thy-1 antigen of the lymphocyte plasmic membrane and not associated with homology of the enzyme primary structure and the alpha 1-thymosin structure.

[Effect of ribonucleases on humoral immunity of experimental animals]. / Vliianie ribonukleaz na gumoral'nyi immunitet éksperimental'nykh zhivotnykh.

The stimulating effect of RNAases on the humoral immune response was observed in experiments with animals. It was shown that the stimulation was mainly mediated by the system of T-lymphocytes. In the T-lymphocyte system positive sensitivity to the enzymes was attributed to the T-helper cell subpopulation.

[Experimental study of the allergenic and modulating properties of RNAses]. / Eksperimental'noe izuchenie allergennykh i moduliruiushchikh svoistv RNKaz.

It was shown with the immediate type hypersensitivity (ITH) test and the delayed type hypersensitivity (DTH) test that pancreatic RNase and RNases from Bacillus sp. had rather pronounced allergenic properties which were similar in both the tests. The modulating properties of the RNases with respect to sensitization in accordance with ITH or DTH were also investigated. It was shown that the enzymes did not influence development of ITH in the experimental animals sensitized with the BCA vaccine. However, they markedly increased DTH in the experimental animals sensitized with the BCG vaccine. RNase from Bacillus sp. selectively inactivated with respect to histidine of the active site maintained its capacity for increasing DTH when used as a native enzyme.

[Structural and antigenic differences of pancreatic RNAse preparations modified by dextran ethers in an azo-combination reaction]. / Strukturnye i antigennye razlichiia preparatov pankreaticheskoi RNKazy, modifitsirovannoi éfirami dekstrana metodom azosochetaniia.

Preparations of pancreatic RNAase modified by dextrane derivatives were obtained in an azocombination reaction. The UV absorption spectra and amino acid analysis of the preparations revealed quantitative and qualitative differences in the sites of the enzyme binding to the polymeric matrix depending on modification conditions. The observed differences in the binding sites of modified RNAase may be related both to the differences in the primary structure or even in the secondary and ternary structure of the enzyme. The latter observation was confirmed in studies of the antigenic properties of modified preparations. The interrelationship of these preparations with antibodies raised against native RNAase and the comparison of the degree of their antigenicity suggest the influence of modification on the antigenic properties of the protein. The number of binding sites of the enzyme to the support can be determined, which would neutralize the antigen-stimulated effect of the support due to the screening of protein antigenic determinants.