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Biomed Res Int ; 2017: 3756089, 2017.
Article in English | MEDLINE | ID: mdl-28357399

ABSTRACT

Deletion of PI3K catalytic subunit p110α in adipose tissue (aP2-Cre/p110αflx/flx, α-/- hereafter) results in increased adiposity, glucose intolerance, and liver steatosis. Because this endocrine organ releases hormones like leptin, which are important in reproductive physiology, we investigated the reproductive phenotype of α-/- males. Compared to controls, α-/- males displayed delayed onset of puberty accompanied by a reduction in plasma LH levels and testicular weight. At postnatal day 30, α-/- mice exhibited normal body weight but elevated fasted plasma leptin levels. Testicular leptin gene expression was increased, whereas expression of the cholesterol transporter StAR and of P450 cholesterol side chain cleavage enzyme was decreased. Adult α-/- males were infertile and exhibited hyperandrogenemia with normal basal LH, FSH, and estradiol levels. However, neither sperm counts nor sperm motility was different between genotypes. The mRNA levels of leptin and of 17-beta-dehydrogenase 3, and enzyme important for testosterone production, were significantly higher in the testis of adult α-/- males. The mRNA levels of ERα, an important regulator of intratesticular steroidogenesis, were lower in the testis of adult and peripubertal α-/- males. We propose that chronic hyperleptinemia contributes to the negative impact that disrupting PI3K signaling in adipocytes has on puberty onset, steroidogenesis, and fertility in males.


Subject(s)
Adipose Tissue/metabolism , Class I Phosphatidylinositol 3-Kinases/genetics , Infertility, Male/genetics , Puberty, Delayed/genetics , 17-Hydroxysteroid Dehydrogenases/biosynthesis , 17-Hydroxysteroid Dehydrogenases/blood , Adipose Tissue/pathology , Animals , Class I Phosphatidylinositol 3-Kinases/biosynthesis , Follicle Stimulating Hormone/blood , Gene Expression Regulation , Genotype , Humans , Infertility, Male/blood , Infertility, Male/pathology , Leptin/blood , Leptin/genetics , Luteinizing Hormone/blood , Male , Mice , Mice, Transgenic , Puberty, Delayed/blood , Puberty, Delayed/pathology , Sperm Count , Sperm Motility/genetics , Testosterone/biosynthesis
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