Realizing the Principles for Remote and Selective Detection of Cancer Cells Using Magnetic Nanowires.

The unmet demand for selective and remote detection of biological entities has urged nanobiotechnology to prioritize the innovation of biolabels that can be remotely detected. Magnetic nanowires (MNWs) have been deemed promising for remote detection as the magnetic fields can deeply and safely penetrate into tissue. However, the overlapping nature of the magnetic signatures has been a long-standing challenge for selective detection, which we resolve here. To do so, 13 types of MNWs with unique irreversible switching field (ISF) signatures were synthesized for labeling canine osteosarcoma (OSCA-8) cancer cells (one set) and polycarbonate biopolymers (12 sets). After characterizing the ISF signature of each MNW type, the MNW-labeled cancer cells were transferred onto MNW-labeled biopolymers to determine the most distinguishable ISF signatures and to discern the principles for reliable selective detection of biological entities. We show that tailoring the ISF of MNWs by tuning their coercivity is a highly effective approach for generating distinct magnetic biolabels for selective detection of cells. These findings smooth the path for the progression of nanobiotechnology by enabling the remote and selective detection of biological entities using MNWs.

Iron Oxide Nanorings and Nanotubes for Magnetic Hyperthermia: The Problem of Intraparticle Interactions.

Magnetic interactions can play an important role in the heating efficiency of magnetic nanoparticles. Although most of the time interparticle magnetic interactions are a dominant source, in specific cases such as multigranular nanostructures intraparticle interactions are also relevant and their effect is significant. In this work, we have prepared two different multigranular magnetic nanostructures of iron oxide, nanorings (NRs) and nanotubes (NTs), with a similar thickness but different lengths (55 nm for NRs and 470 nm for NTs). In this way, we find that the NTs present stronger intraparticle interactions than the NRs. Magnetometry and transverse susceptibility measurements show that the NTs possess a higher effective anisotropy and saturation magnetization. Despite this, the AC hysteresis loops obtained for the NRs (0-400 Oe, 300 kHz) are more squared, therefore giving rise to a higher heating efficiency (maximum specific absorption rate, SARmax = 110 W/g for the NRs and 80 W/g for the NTs at 400 Oe and 300 kHz). These results indicate that the weaker intraparticle interactions in the case of the NRs are in favor of magnetic hyperthermia in comparison with the NTs.

Selective Detection of Cancer Cells Using Magnetic Nanowires.

The main bottleneck for implementing magnetic nanowires (MNWs) in cell-biology research for multimodal therapeutics is the inapplicability of the current state of the art for selective detection and stimulation of MNWs. Here, we introduce a methodology for selective detection of MNWs in platforms that have multiple magnetic signals, such as future multimodal therapeutics. After characterizing the signatures of MNWs, MNWs were surface-functionalized and internalized into canine osteosarcoma (OSCA-8) cancer cells for cell labeling, manipulation, and separation. We also prepared and characterized magnetic biopolymers as multimodal platforms for future use in controlling the movement, growth, and division of cancer cells. First, it is important to have methods for distinguishing the magnetic signature of the biopolymer from the magnetically labeled cells. For this purpose, we use the projection method to selectively detect and demultiplex the magnetic signatures of MNWs inside cells from those inside magnetic biopolymers. We show that tailoring the irreversible switching field of MNWs by tuning their coercivity is a highly effective approach for generating distinct magnetic biolabels for selective detection of cancer cells. These findings open up new possibilities for selective stimulation of MNWs in multimodal therapeutic platforms for drug delivery, hyperthermia cancer therapy, and mitigating cancer cell movement and proliferation.

Isolation of Cancer-Derived Exosomes Using a Variety of Magnetic Nanostructures: From Fe3O4 Nanoparticles to Ni Nanowires.

Isolating and analyzing tumor-derived exosomes (TEX) can provide important information about the state of a tumor, facilitating early diagnosis and prognosis. Since current isolation methods are mostly laborious and expensive, we propose herein a fast and cost-effective method based on a magnetic nanoplatform to isolate TEX. In this work, we have tested our method using three magnetic nanostructures: (i) Ni magnetic nanowires (MNWs) (1500 × 40 nm), (ii) Fe3O4 nanorods (NRs) (41 × 7 nm), and (iii) Fe3O4 cube-octahedral magnetosomes (MGs) (45 nm) obtained from magnetotactic bacteria. The magnetic response of these nanostructures has been characterized, and we have followed their internalization inside canine osteosarcoma OSCA-8 cells. An overall depiction has been obtained using a combination of Fluorescence and Scanning Electron Microscopies. In addition, Transmission Electron Microscopy images have shown that the nanostructures, with different signs of degradation, ended up being incorporated in endosomal compartments inside the cells. Small intra-endosomal vesicles that could be precursors for TEX have also been identified. Finally, TEX have been isolated using our magnetic isolation method and analyzed with a Nanoparticle tracking analyzer (NanoSight). We observed that the amount and purity of TEX isolated magnetically with MNWs was higher than with NRs and MGs, and they were close to the results obtained using conventional non-magnetic isolation methods.

Exchange Bias Effects in Iron Oxide-Based Nanoparticle Systems.

The exploration of exchange bias (EB) on the nanoscale provides a novel approach to improving the anisotropic properties of magnetic nanoparticles for prospective applications in nanospintronics and nanomedicine. However, the physical origin of EB is not fully understood. Recent advances in chemical synthesis provide a unique opportunity to explore EB in a variety of iron oxide-based nanostructures ranging from core/shell to hollow and hybrid composite nanoparticles. Experimental and atomistic Monte Carlo studies have shed light on the roles of interface and surface spins in these nanosystems. This review paper aims to provide a thorough understanding of the EB and related phenomena in iron oxide-based nanoparticle systems, knowledge of which is essential to tune the anisotropic magnetic properties of exchange-coupled nanoparticle systems for potential applications.