ABSTRACT
The influence of ceruloplasmin on cell proliferation in regeneration liver of the rat has been studied. Ceruloplasmin stimulates cell proliferation in regeneration liver increasing functional activity of the mononuclear phagocytes.
Subject(s)
Ceruloplasmin/pharmacology , Liver Regeneration/drug effects , Liver/drug effects , 5'-Nucleotidase/analysis , 5'-Nucleotidase/drug effects , Adenosine Deaminase/analysis , Adenosine Deaminase/drug effects , Animals , Ascitic Fluid/cytology , Cell Division/drug effects , Hepatectomy , Liver/cytology , Macrophages/drug effects , Macrophages/enzymology , Male , Rats , Time FactorsABSTRACT
Hepatocytes in cell culture have been studied for their sensitivity to mitogenic effect of the epidermal growth factor, epidermal growth factor and insulin, 10% of cattle blood serum after hyperthermia. It is established that heat shock (43 degrees C, 90 min) induces hypersensitivity of hepatocytes to the mentioned mitogens, its maximum being observed 20-24 h after hyperthermia and coinciding in time with the development of thermotolerance and intensification of protein biosynthesis after the heat shock 68, 70, 94 kD.
Subject(s)
Heat-Shock Proteins/biosynthesis , Hyperthermia, Induced , Liver/cytology , Animals , Cell Division , Cells, Cultured , Epidermal Growth Factor/pharmacology , Insulin/pharmacology , Male , Mitogens/pharmacology , Rats , Time FactorsABSTRACT
It was established that difluoromethylornithine (DFMO) and methylglyoxal-bis(guanylhydrazone) blocked the mitogenic response of hepatocytes to epidermal growth factor. This effect of inhibitors, especially DFMO, has been prevented by exogenic putrescine, spermidine or spermine. But neither blocking action of inhibitors nor preventing effect of polyamines associated with changes of epidermal growth factor binding to hepatocytes.
Subject(s)
Biogenic Polyamines/antagonists & inhibitors , Epidermal Growth Factor/pharmacology , Liver/drug effects , Mitosis/drug effects , Polyamines/pharmacology , Animals , Biogenic Polyamines/biosynthesis , Cells, Cultured , DNA/biosynthesis , DNA/drug effects , Drug Interactions , Eflornithine/pharmacology , Mitoguazone/pharmacologyABSTRACT
It is established that sialadenectomy leads to inhibition of hepatocarcinogenesis induced in adult rats by diethylnitrosamine, which is confirmed by a decrease in the incidence of tumours, their delayed growth and an increase of longevity. But sialadenectomy 2 weeks after the beginning of carcinogen treatment has no effect on the hepatocarcinogenesis. A significant molecular heterogeneity of the epidermal growth factor-competing activity is observed in acid-ethanol extracts of the liver tumour of the sialadenectomized rats.
