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1.
Biophys J ; 78(6): 3081-92, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10827985

ABSTRACT

In striated muscle, force generation and phosphate (P(i)) release are closely related. Alterations in the [P(i)] bathing skinned fibers have been used to probe key transitions of the mechanochemical coupling. Accuracy in this kind of studies is reduced, however, by diffusional barriers. A new perfusion technique is used to study the effect of [P(i)] in single or very thin bundles (1-3 microM in diameter; 5 degrees C) of rabbit psoas myofibrils. With this technique, it is possible to rapidly jump [P(i)] during contraction and observe the transient and steady-state effects on force of both an increase and a decrease in [P(i)]. Steady-state isometric force decreases linearly with an increase in log[P(i)] in the range 500 microM to 10 mM (slope -0.4/decade). Between 5 and 200 microM P(i), the slope of the relation is smaller ( approximately -0.07/decade). The rate constant of force development (k(TR)) increases with an increase in [P(i)] over the same concentration range. After rapid jumps in [P(i)], the kinetics of both the force decrease with an increase in [P(i)] (k(Pi(+))) and the force increase with a decrease in [P(i)] (k(Pi(-))) were measured. As observed in skinned fibers with caged P(i), k(Pi(+)) is about three to four times higher than k(TR), strongly dependent on final [P(i)], and scarcely modulated by the activation level. Unexpectedly, the kinetics of force increase after jumps from high to low [P(i)] is slower: k(Pi(-)) is indistinguishable from k(TR) measured at the same [P(i)] and has the same calcium sensitivity.


Subject(s)
Muscle Contraction/drug effects , Muscle, Skeletal/physiology , Myofibrils/physiology , Phosphates/pharmacology , Adenosine Diphosphate/pharmacology , Animals , In Vitro Techniques , Kinetics , Muscle Fibers, Fast-Twitch/drug effects , Muscle Fibers, Fast-Twitch/physiology , Muscle, Skeletal/drug effects , Myofibrils/drug effects , Rabbits
2.
J Physiol ; 516 ( Pt 3): 847-53, 1999 May 01.
Article in English | MEDLINE | ID: mdl-10200430

ABSTRACT

1. The effects of magnesium adenosine triphosphate (MgATP; also referred to as 'substrate') concentration on maximal force and shortening velocity have been studied at 5 C in single and thin bundles of striated muscle myofibrils. The minute diameters of the preparations promote rapid diffusional equilibrium between the bathing medium and lattice space so that during contraction fine control of substrate and product concentrations is achieved. 2. Myofibrils from frog tibialis anterior and rabbit psoas fast skeletal muscles were activated maximally by rapidly (10 ms) exchanging a continuous flux of pCa 8.0 for one at pCa 4.75 at a range of substrate concentrations from 10 microM to 5 mM. At high substrate concentrations maximal isometric tension and shortening velocity of both frog and rabbit myofibrils were very close to those determined in whole fibre preparations from the same muscle types. 3. As in frog and rabbit skinned whole fibres, the maximal isometric force of the myofibril preparations decreases as MgATP concentration is increased. The maximal velocity of unloaded shortening (V0) depends hyperbolically on substrate concentration. V0 extrapolated to infinite MgATP (3.6 +/- 0.2 and 0.8 +/- 0.03 l0 s-1 in frog and rabbit myofibrils, respectively) is very close to that determined directly at high substrate concentration. The Km is 210 +/- 20 microM for frog tibialis anterior and 120 +/- 10 microM for rabbit psoas myofibrils, values about half those found in larger whole fibre preparations of the same muscle types. This implies that measurements in whole skinned fibres are perturbed by diffusional delays, even in the presence of MgATP regenerating systems. 4. In both frog and rabbit myofibrils, the Km for V0 is about one order of magnitude higher than the Km for myofibrillar MgATPase determined biochemically in the same experimental conditions. This confirms that the difference between the Km values for MgATPase and shortening velocity is a basic feature of the mechanism of chemomechanical transduction in muscle contraction.


Subject(s)
Muscle, Skeletal/physiology , Myofibrils/physiology , Adenosine Triphosphate/pharmacology , Animals , In Vitro Techniques , Isometric Contraction/drug effects , Isometric Contraction/physiology , Kinetics , Muscle, Skeletal/cytology , Muscle, Skeletal/drug effects , Myofibrils/drug effects , Rabbits , Rana esculenta
3.
Adv Exp Med Biol ; 453: 373-81; discussion 381-2, 1998.
Article in English | MEDLINE | ID: mdl-9889849

ABSTRACT

Single myofibrils or small groups of myofibrils were isolated from different types of striated muscle: rabbit psoas, frog tibialis anterior, frog atrial and ventricular muscle. The Ca2+ concentration of the solution perfusing the myofibrils was changed within few milliseconds by translating the interface between two flowing streams of solution across the preparations. In all types of myofibrils tested, the time course of force rise in response to maximal activation (pCa 4.75) was approximately monoexponential and nearly superimposable on that observed after a release-restretch protocol applied to the myofibril at the plateau of maximal contractions. This suggests that the kinetics of force development following rapid myofibril activation essentially reflects the kinetics of interaction between contractile proteins. The half time of force rise in response to maximal activation varied among different myofibril types; it was shortest in frog tibialis anterior myofibrils and longest in frog ventricular myofibrils. In all types of myofibril preparations tested the half time of force rise increased with decreasing Ca2+ levels in the activating solution. The finding provides support for a kinetic mechanism of force regulation by Ca2+ in all types of striated muscle. The extent of this Ca2+ effect, however, varied among the different myofibril preparations tested; at 15 degrees C for instance, it was smaller in frog tibialis anterior myofibrils than in the other preparations.


Subject(s)
Calcium/physiology , Muscle Contraction , Muscle Fibers, Skeletal/physiology , Muscle, Skeletal/physiology , Animals , Rabbits , Rana esculenta
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