ABSTRACT
The method for the two-cycle cultivation of V. cholerae, reference strains Inaba and Ogawa, in a fermenter has been developed. In the first and second cultivation cycles no difference in the yield of the biomass and in the ultrastructure of the vibrios has been established. In the second cycle a decrease in the minimum time of generation has been observed. The vaccines obtained in the first and second cultivation cycles have not been found to differ in their antigenic and immunogenic activity. The vaccines meet the WHO requirements.
Subject(s)
Fermentation , Vibrio cholerae/growth & development , Animals , Antibodies, Bacterial/analysis , Antigens, Bacterial/immunology , Bacterial Vaccines/immunology , Bacteriological Techniques/instrumentation , Mice , Microscopy, Electron , Time Factors , Vibrio cholerae/immunology , Vibrio cholerae/ultrastructureABSTRACT
The cultivation of V. cholerae was carried out in casein broth in a fermenter. 2 kinds of cultivation conditions were compared. The results thus obtained indicate that the cultivation of V. cholerae under the conditions of the controlled level of dissolved oxygen (pO2--50%) ensures the optimum conditions for proliferation and the high yield of biomass; it also allows obtaining vaccine whose immunogenic properties are not inferior to those of the WHO reference vaccine.
