ABSTRACT
AIM: Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014. MATERIALS AND METHODS: Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional-methods. RESULTS: The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili of adhesion, contained genes of hemagglutinin/protease, protease PrtV, collagenase, cytotonic factor Cef, outer membrane protein-OmpW, tol- and -vps-clusters, regulatory genes toxR and hapR. Antibioticogramms of the strains have shown the presence of cultures, resistant to ampicillin, ceftazidime-furazolidone, trimethoprim/sulfamethoxazole with intermediate resistance to streptomycin, kanamycin, gentamycin, amikacin, netilmicin, Approximately 20% of isolates had multiple drug resistance. Data of VNTR- and genotyping confirmed a possibility of water transmission route of the infection. CONCLUSION: Execution of monitoring of cultures from environmental samples is necessary for timely detection of genetic characteristics, antibiotics resistance.
Subject(s)
Cholera/epidemiology , Genes, Bacterial , Vibrio cholerae O139/genetics , Vibrio cholerae non-O1/genetics , Water Microbiology , Anti-Bacterial Agents/pharmacology , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cholera/drug therapy , Cholera/microbiology , Cholera/transmission , Cholera Toxin/genetics , Cholera Toxin/metabolism , Collagenases/genetics , Collagenases/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Drug Resistance, Multiple, Bacterial , Epidemiological Monitoring , Fimbriae, Bacterial , Gene Deletion , Humans , Immune Sera/chemistry , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , Microbial Sensitivity Tests , Peptide Hydrolases/genetics , Peptide Hydrolases/metabolism , Phylogeny , Russia/epidemiology , Serotyping , Transcription Factors/genetics , Transcription Factors/metabolism , Vibrio cholerae O139/classification , Vibrio cholerae O139/drug effects , Vibrio cholerae O139/isolation & purification , Vibrio cholerae non-O1/classification , Vibrio cholerae non-O1/drug effects , Vibrio cholerae non-O1/isolation & purificationABSTRACT
AIM: Application of the authors' GIS <
