Subject(s)
Bradycardia/etiology , CD40 Ligand/analysis , Erythroblastosis, Fetal/prevention & control , Heart Rate, Fetal/drug effects , Platelet Transfusion/adverse effects , Pregnancy Outcome , Adult , Biomarkers/blood , Blood Donors , Blood Transfusion, Intrauterine/adverse effects , Blood Transfusion, Intrauterine/methods , Bradycardia/diagnosis , Female , Follow-Up Studies , Gestational Age , Humans , Infant, Newborn , Platelet Transfusion/methods , Pregnancy , Risk Assessment , Sensitivity and Specificity , Treatment OutcomeABSTRACT
The human neutrophil antigens HNA-1a, -1b and -1c play an important role in immune neutropenia. The frequencies of the coding FCGR3B genes were determined in different populations. New FCGR3B variants were also found in some populations. This study investigated the FCGR3B gene frequencies and FCGR3 variants in a Chinese population compared with the results of Northern Germans and African Blacks (Uganda). Our results show that the gene frequencies in 413 healthy Chinese individuals from Zhejiang Province were 0.565 for FCGR3B*1, 0.430 for FCGR3B*2 and 0.00 for FCGR3B*3. The genotype frequency of FCGR3B(null) was 0.48% (2/413). Sequencing of FCGR3 revealed that in seven out of 19 Chinese individuals, cloned and sequenced DNA fragments that exhibited variants caused by single nucleotide exchanges at one or more of the polymorphic positions 141, 147, 227, 266 and 277 in exon 3 also existed in this Chinese population. From the present study, it is concluded that the FCGR3B*1 gene is more frequent in a Chinese population from Zhejiang Province than the FCGR3B*2 gene, and the FCGR3B*3 gene seems to be absent, which is in contrast to studies in the white populations. Gene variants caused by single nucleotide exchanges were found in addition to the well-known forms, but the reason for this remains unclear.
Subject(s)
Gene Frequency , Genetic Variation , Isoantigens/genetics , Alleles , Antigens, CD , Asian People/genetics , Black People/genetics , China , DNA/blood , DNA/chemistry , GPI-Linked Proteins , Genotype , Humans , Polymerase Chain Reaction , Polymorphism, Genetic , Receptors, IgG , Sequence Analysis, DNA , White People/geneticsABSTRACT
Fcgamma receptor (FcgammaR)-mediated destruction of immunoglobulin-coated red blood cells (RBCs) is the underlying mechanism of haemolytic disease of the newborn. Human leucocyte antigen (HLA) antibodies in vitro are able to block monocyte FcgammaRs and prevent phagocytosis. The intention was to demonstrate this effect in vivo upon a volunteer. Plasma containing a non-cytotoxic HLA-DR alloantibody was infused into the subject. The FcgammaR blockade was achieved and persisted for about 2.5 d, but, unexpectedly, a mild transfusion-related acute lung injury (TRALI) was also caused. Monocytes disappeared completely from the peripheral blood within the first hour after infusion and a mild pulmonary oedema was observed within 3-4 h. The subject recovered within 2 d.
