Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-100 and Polyvinylpyrrolidone.

We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Ð¥-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 µ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Ð¥-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Ð¥-100 is more favorable for population with cells.

[Palivizumab: four seasons in Russia].

In 2010, the Russian Federation (RF) registered palivizumab--innovative drug, based on monoclonal antibodies for passive immunization of seasonal respiratory syncytial virus (RSV) infection in children of disease severe progress risk group, which include primarily premature infants, children with bronchopulmonary dysplasia and hemodynamically significant congenital heart disease. Currently, palivizumab is included in the list of recommended medicines and medical care standards of different countries, including Russia. In the review the results of Russian research on the progress of RSV infection, its epidemiology and immunization experience gained over the 2010-2014 period are summarized in relation to the foreign data. During the four epidemic seasons palivizumab immunization covered more than 3,200 children of severe RSV infection risk group with a progressive annual increase in the number of patients who received the drug. Geography of palivizumab immunization is also greatly expanded in our country during this time. If during the first two seasons measures of immunization were taken mainly in Moscow and St. Petersburg, at the present time, thirty one territorial entities of the Russian Federation have the experience in the drug application. Analysis of the results of RSV infection immunization (made in several regions) confirms the high clinical efficacy and palivizumab safety already demonstrated in international studies. In addition, the analysis presents the potential to improve the efficiency of the integrated RSV infection immunization programs, realizing in the establishment of high-risk child group register, adequate counseling for parents, as well as the development of the routing of patients and coordination of interaction between different health institutions during the immunization.

[Insulin-glucose parameters in healthy women and patients with arterial hypertension and ischemic heart disease in dependence on age and follicle-stimulating hormone levels].

Insulin-glucose parameters in healthy women do not significantly change with changes of functional state of ovaries. Women with hypertension and normal levels of glycemia have significantly higher values of basal insulinemia and HOMA-R index reflecting presence of moderate insulin resistance degree of which is more pronounced in middle aged patients. In women with documented ischemic heart disease values of basal insulinemia, HOMA-R index, and glycemia are comparable with those in patients with hypertension and follicle-stimulating hormone level above 30 IE/l and significantly differ from corresponding parameters in healthy women in peri- and postmenopause.

[Hormone-producing function of the ovaries in young and middle-aged women with arterial hypertension and coronary heart disease].

The study demonstrates that arterial hypertension itself, judging by main anthropometric variables such as body weight index and waist to hip ratio, together with the estrogen-producing function of the ovaries, evaluated by measuring follicle-stimulating hormone levels, contributes substantially to the forming of abdominal obesity in normo- and hypertensive women. All the above confirms close correlation between hemodynamic and metabolic disturbances which eventually result in coronary heart disease in young and middle-aged women.

[On the possible prooxidant role of insulinemia in formation of metabolic syndrome in men and women.]. / O vozmozhnoi prooksidantnoi roli insulinemii v formirovanii metabolicheskogo sindroma u muzhchin i zhenshchin.

Levels of basal insulinemia, malondialdehyde (an index of oxidative stress) and some metabolic parameters were studied in 143 men and 83 women aged 48.3+/-0.7 years with incomplete metabolic syndrome: hypertension, abdominal obesity, dyslipoproteinemia with normoglycemia. Basal insulinemia correlated significantly with malondialdehyde levels both in men and women. Slose relationship was also found between basal insulinemia and waist circumference.

[Myocardial infarction in women: risk factors and clinical features]. / Faktory riska i osobennosti techeniia infarkta miokarda u zhenshchin.

The course of myocardial infarction (MI) in women, especially 60 years of age and older, is characterized by such severe complications as cardiorrhexis, hypovolemic cardiogenic shock, asystole, recurrent ventricular fibrillation and electromechanic dissociation responsible for the majority of lethal outcomes. Especially high MI lethality is in women at the age 70-79 years who have also the highest incidence of recurrent macrofocal MI while small-focal MI occurs in women over 80 years of age (80-89) more frequently than in 60-year-olds and younger. Dominating MI risk factors in women were the following: arterial hypertension detected in 81% patients under 60 and 90.8% cases over 60 years; abnormal lipid spectrum of blood including hypercholesterolemia (HCE), hypertriglyceridemia (HTE) and low concentration of HDLP cholesterol. HCE and HTE closely correlated with abdominal obesity irrespectively of age. Early menopause in women under 60 and diabetes mellitus of type 2 in older women, accumulation of two and more factors of risk contribute to development of coronary heart disease and MI, in females.