ABSTRACT
Modic changes (MCh) are pathological changes in the bone marrow and the endplates of adjacent vertebrae characterized by bone marrow edema, fatty degeneration or osteosclerosis that are revealed by high-power field magnetic resonance imaging (MRI). The dominant disease pattern is a persistent back pain syndrome with varying intensity. It leads to difficulties in the interpretation of clinical data, evaluation of MRI data and treatment. The article presents a modern conception of the pathogenesis, classification, clinical implications and treatment of MCh based on national and foreign sources as well and the author's view on the osteogenic mechanism of the pathogenesis and treatment of MCh using intraosseous blockades.
Subject(s)
Low Back Pain , Lumbar Vertebrae/pathology , Adult , Bone Marrow/pathology , Female , Humans , Low Back Pain/classification , Low Back Pain/diagnosis , Low Back Pain/pathology , Low Back Pain/therapy , Magnetic Resonance Imaging , Male , Middle AgedABSTRACT
The protein spectra for 70 strains of obligate methanotrophic bacteria were studied using electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. The protein spectra of Methylomonas methanica 12 and Methylosinus trichosporium 44 did not change in the course of the culture growth. Basing on the data obtained, the similarity coefficients were calculated for these strains. The numerical analysis of the similarity coefficients was done, and a dendrogram presenting the phylogenetic relationships between these bacterial strains, species, genera and groups was constructed. The coefficients of similarity between the strains of one species varied within the range of 78 to 100%. The similarity coefficients of the species and genera varied from 36 to 38%, respectively. The data suggest the existence of 5 independent genera of obligate methanotrophic bacteria: Methylomonas, Methylobacter, Methylococcus, Methylosinus and Methylocystis.
Subject(s)
Fungal Proteins/classification , Methylococcaceae/classification , Electrophoresis, Polyacrylamide Gel/methods , Fungal Proteins/analysis , Methylococcaceae/analysis , PhylogenySubject(s)
Coal Mining , Methylococcaceae/metabolism , Water Microbiology , Methane/metabolism , Oxidation-Reduction , UkraineSubject(s)
Rheumatic Diseases , Clinical Trials as Topic , Collagen Diseases , Humans , Research , USSRABSTRACT
Growth parameters and their interrelationship were determined for two mixed and seven pure cultures of methanotrophic bacteria. The specific growth rate, the rate of methane assimilation, and the economic coefficient changed significantly in the course of cultivation of the methanotrophic cultures in the periodic regime, the changes being of the extremem character. Therefore, mean values of the growth parameters of these cultures determined under identical conditions were compared. These parameters were found to be dependent on the taxonomy of the cultures and on the conditions of their growth. No considerable differences had been found in the growth parameters between mixed and pure methanotrophous cultures. Portions of the carbon of methane expended for synthesis of the biomass, carbon dioxide, and exometabolites was different among methanotrophic cultures belonging to different genera.
Subject(s)
Bacteria/growth & development , Methane/metabolism , Bacteria/metabolism , Species SpecificitySubject(s)
Joint Diseases/classification , Terminology as Topic , Adult , Child , Humans , Rheumatic Diseases/classificationABSTRACT
Adsorption of pure cultures of methane oxidizing bacteria, Methylosinus trichosporium 20 and Methylococcus ucrainicus 21, on glass and coal was studied; the former strain was sorbed on both sorbents, the latter strain was sorbed on coal but not on glass. The rate of methane oxidation by the cells of adsorbed microorganisms was higher than in the case of free cells, and increased with a decrease in dimensions of the sorbent particles.
Subject(s)
Gram-Negative Aerobic Bacteria/metabolism , Methane/metabolism , Adsorption , Chromatography, Gas , Coal , Culture Media , Glass , Time FactorsABSTRACT
Respiration of the cells of Methylococcus ucrainicus, strain 21, cultivated in the atmosphere of methane, is stimulated by methanol, formaldehyde, formate, n-alcohols, and allyl alcohol. The rate of oxygen assimilation is lower in the presence of isopropanol, isobutanol, propane, butane, maltose, and some organic acids (acetate, fumarate, citrate, succinate). The Michaelis constant for methanol is 88 mcM. Oxidation of methane, methanol, formaldehyde, and formate by the bacterium is inhibited by cyanide, hydroxylamine, and azide. The rate of oxygen assimilation by the cells in the presence of methane and other C1-compounds did not decrease after the suspension had been stored at 4 degrees C during four months and longer.
Subject(s)
Gram-Negative Aerobic Bacteria/metabolism , Methane/metabolism , Oxygen Consumption , Culture Media , Gases/metabolism , Methanol/metabolism , Oxidation-Reduction/drug effects , Oxygen Consumption/drug effectsABSTRACT
The efficacy of disrupting cells of methane oxidizing bacteria M. trichosporium strain 20 by air decompression was measured. The efficacy increased with a rise of the gas pressure, density of the bacterial suspension and the ratio of the air and suspension volumes. The relationship between the efficacy of disruption of the microorganisms and the rate of pressure reduction showed a distinct maximum. The efficacy of the bacterial disruption did not change with an increase of the time of saturation of the bacterial suspension by air from 30 sec. to 60 min.
