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1.
Nat Aging ; 1(8): 666-676, 2021 08.
Article in English | MEDLINE | ID: mdl-37117767

ABSTRACT

The gut microbiota is increasingly recognized as an important regulator of host immunity and brain health. The aging process yields dramatic alterations in the microbiota, which is linked to poorer health and frailty in elderly populations. However, there is limited evidence for a mechanistic role of the gut microbiota in brain health and neuroimmunity during aging processes. Therefore, we conducted fecal microbiota transplantation from either young (3-4 months) or old (19-20 months) donor mice into aged recipient mice (19-20 months). Transplant of a microbiota from young donors reversed aging-associated differences in peripheral and brain immunity, as well as the hippocampal metabolome and transcriptome of aging recipient mice. Finally, the young donor-derived microbiota attenuated selective age-associated impairments in cognitive behavior when transplanted into an aged host. Our results reveal that the microbiome may be a suitable therapeutic target to promote healthy aging.


Subject(s)
Gastrointestinal Microbiome , Microbiota , Animals , Mice , Fecal Microbiota Transplantation , Aging/genetics , Brain
2.
Nutrients ; 12(9)2020 Sep 12.
Article in English | MEDLINE | ID: mdl-32932733

ABSTRACT

Blueberry (BB) consumption is linked to improved health. The bioconversion of the polyphenolic content of BB by fermentative bacteria in the large intestine may be a necessary step for the health benefits attributed to BB consumption. The identification of specific gut microbiota taxa that respond to BB consumption and that mediate the bioconversion of consumed polyphenolic compounds into bioactive forms is required to improve our understanding of how polyphenols impact human health. We tested the ability of polyphenol-rich fractions purified from whole BB-namely, anthocyanins/flavonol glycosides (ANTH/FLAV), proanthocyanidins (PACs), the sugar/acid fraction (S/A), and total polyphenols (TPP)-to modulate the fecal microbiota composition of healthy adults in an in vitro colon system. In a parallel pilot study, we tested the effect of consuming 38 g of freeze-dried BB powder per day for 6 weeks on the fecal microbiota of 17 women in two age groups (i.e., young and older). The BB ingredients had a distinct effect on the fecal microbiota composition in the artificial colon model. The ANTH/FLAV and PAC fractions were more effective in promoting microbiome alpha diversity compared to S/A and TPP, and these effects were attributed to differentially responsive taxa. Dietary enrichment with BB resulted in a moderate increase in the diversity of the microbiota of the older subjects but not in younger subjects, and certain health-relevant taxa were significantly associated with BB consumption. Alterations in the abundance of some gut bacteria correlated not only with BB consumption but also with increased antioxidant activity in blood. Collectively, these pilot data support the notion that BB consumption is associated with gut microbiota changes and health benefits.


Subject(s)
Blueberry Plants/chemistry , Gastrointestinal Microbiome/drug effects , Polyphenols/pharmacology , Adult , Aged , Anthocyanins/pharmacology , Antioxidants/metabolism , Colon/microbiology , Feces/microbiology , Female , Fermentation , Flavonols/pharmacology , Glycosides/pharmacology , Healthy Volunteers , Humans , Models, Anatomic , Oxidative Stress/drug effects , Pilot Projects , Young Adult
3.
ISME J ; 11(9): 2059-2074, 2017 09.
Article in English | MEDLINE | ID: mdl-28585938

ABSTRACT

The biological significance of Archaea in the human gut microbiota is largely unclear. We recently reported genomic and biochemical analyses of the Methanomassiliicoccales, a novel order of methanogenic Archaea dwelling in soil and the animal digestive tract. We now show that these Methanomassiliicoccales are present in published microbiome data sets from eight countries. They are represented by five Operational Taxonomic Units present in at least four cohorts and phylogenetically distributed into two clades. Genes for utilizing trimethylamine (TMA), a bacterial precursor to an atherosclerogenic human metabolite, were present in four of the six novel Methanomassiliicoccales genomes assembled from ELDERMET metagenomes. In addition to increased microbiota TMA production capacity in long-term residential care subjects, abundance of TMA-utilizing Methanomassiliicoccales correlated positively with bacterial gene count for TMA production and negatively with fecal TMA concentrations. The two large Methanomassiliicoccales clades have opposite correlations with host health status in the ELDERMET cohort and putative distinct genomic signatures for gut adaptation.


Subject(s)
Archaea/genetics , Archaea/metabolism , Gastrointestinal Microbiome , Methylamines/metabolism , Aged , Aged, 80 and over , Animals , Archaea/classification , Archaea/isolation & purification , Feces/microbiology , Female , Gastrointestinal Tract/microbiology , Genomics , Humans , Male , Metagenome , Metagenomics , Microbiota , Phylogeny
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