ABSTRACT
Oligomer 12 S alpha-toxin as well as 3 S alpha-toxoid of Staphylococcus aureus induced the formation of monoclonal antibodies (mabs). Mabs against the 12 S alpha-toxin could be demonstrated in 31 and those against 3 S alpha-toxoid in 18 of 120 hybrid cell colonies. Each of these mab-preparations reacted with 12 S, 3 S alpha-toxin and 3 S alpha-toxoid. The reactions were more pronounced with the homologous than the heterologous toxin preparations. Mabs against 12 S alpha-toxin inhibited the hemolytic effects of native 3 S alpha-toxin as well or better than the respective polyclonal antisera.
Subject(s)
Antibodies, Monoclonal , Bacterial Toxins/pharmacology , Hemolysin Proteins , Hemolysis/drug effects , Animals , Antigen-Antibody Complex , Bacterial Toxins/immunology , Bacterial Toxins/isolation & purification , Hybridomas/immunology , Kinetics , Mice , Staphylococcus aureus/analysisSubject(s)
Bacterial Toxins/immunology , Bacterial Vaccines/immunology , Hemolysin Proteins/immunology , Rabbits , Staphylococcal Infections/veterinary , Staphylococcus aureus/immunology , Animals , Injections, Intradermal/veterinary , Staphylococcal Infections/prevention & control , Vaccination/veterinaryABSTRACT
Concentrations of alpha 2-macroglobulin could be determined in the sera of 215 blood donors and 94 patients with various internal diseases by quantitative inhibition of an acid protease from Staphylococcus aureus, M 135 (fig. 1, 2). The determinations agreed closely with those obtained by immunodiffusion (tab. 1, fig. 3). However, the alpha 2-macroglobulin-measurements by the protease method required only 4 h and 40 microliter serum. This procedure would also be suitable for the determination of alpha 2-macroglobulin in sera from experimental and domestic animals.
