ABSTRACT
The aim of this study was to characterise the immunoglobulin G (IgG) response in 21 dogs with or without pyoderma to antigens from six isolates of Staphylococcus intermedius. The staphylococcal proteins were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis, transferred electrophoretically on to a membrane and subjected to immunoblotting with the dogs' serum. Gels containing separated proteins from the six isolates revealed 29 to 33 distinct bands with molecular weights ranging from 20 to 230 kDa. All the dogs' sera contained IgG that recognised 12 to 24 bands (mean 17), regardless of whether the dogs had pyoderma. The recognised proteins had molecular weights ranging from 20 to 198 kDa but the majority had molecular weights below 75 kDa. The most intense band in all six isolates had a molecular weight of 28 to 29 kDa. The antibody responses to the six isolates were essentially similar except that there were significantly more bands in the response to isolate 2 than to isolate 6, and occasional differences in the intensity of individual bands. All 21 dogs mounted an IgG response to multiple antigens in S intermedius, which differed only marginally between the six isolates. This lack of variation provides evidence that the host's response to different isolates of S intermedius is not a major factor in canine pyoderma.
Subject(s)
Antibodies, Bacterial/blood , Dog Diseases/immunology , Pyoderma/veterinary , Staphylococcal Infections/veterinary , Staphylococcal Skin Infections/veterinary , Staphylococcus/immunology , Animals , Antigens, Bacterial/immunology , Bacterial Proteins/analysis , Dog Diseases/microbiology , Dogs , Electrophoresis, Polyacrylamide Gel/veterinary , Immunoblotting/veterinary , Immunoglobulin G/blood , Molecular Weight , Pyoderma/immunology , Pyoderma/microbiology , Staphylococcal Infections/immunology , Staphylococcal Infections/microbiology , Staphylococcal Skin Infections/immunology , Staphylococcal Skin Infections/microbiologySubject(s)
Dog Diseases/diagnosis , Insecticides/therapeutic use , Mite Infestations/veterinary , Toluidines/therapeutic use , Administration, Topical , Animals , Contraindications , Diagnosis, Differential , Dog Diseases/drug therapy , Dog Diseases/parasitology , Dogs , Female , Mite Infestations/diagnosis , Mite Infestations/drug therapy , Mites/drug effects , Mites/growth & development , Skin/parasitology , Skin/pathology , Tick ControlABSTRACT
A four-year-old, entire male toy poodle was presented with a two-and-a-half-week history of ocular discharge progressing to periorbital alopecia, depigmentation, alopecia and ulceration around the muzzle. There was also a haemorrhagic discharge from the ears, pyrexia, lethargy and generalised lymphadenopathy. The clinical, cytological, bacteriological and histopathological findings were consistent with a diagnosis of dermatitis resembling juvenile cellulitis in an adult dog. Glucocorticoid therapy led to rapid resolution of the clinical signs and the dog has remained in remission for two years after cessation of treatment.
