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1.
Int Arch Allergy Immunol ; 126(1): 68-77, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11641608

ABSTRACT

BACKGROUND: The major birch pollen allergen Bet v 1 represents one of the most prevalent environmental allergens responsible for allergic airway inflammation. OBJECTIVE: In the present study we sought to compare the complete recombinant Bet v 1 allergen molecule with genetically produced hypoallergenic fragments of Bet v 1 regarding mucosal tolerance induction in a mouse model of allergic asthma. METHODS: BALB/c mice were intranasally treated with recombinant Bet v 1 or with two recombinant Bet v 1 fragments (F I: aa 1-74; F II: aa 75-160) prior to aerosol sensitization with birch pollen and Bet v 1. RESULTS: Intranasal application of F II, containing the major T cell epitope, led to significant reduction of IgE/IgG1 antibody responses, in vitro cytokine production (IL-5, IFN-gamma, IL-10) and negative immediate cutaneous hypersensitivity reactions comparable to the pretreatment with the complete rBet v 1 allergen. Moreover, airway inflammation (eosinophilia, IL-5) was inhibited by the pretreatment with either the complete Bet v 1 or F II. However, for prevention of airway hyperresponsiveness the complete molecule was required. The mechanisms leading to immunosuppression seemed to differ in their dependence on the conformation of the molecules, since tolerance induced with the complete Bet v 1, but not with F II, was transferable with spleen cells and associated with increased TGF-beta mRNA levels. CONCLUSION: We conclude that mucosal tolerance induction with recombinant allergens and genetically engineered hypoallergenic derivatives thereof could provide a convenient and safe intervention strategy against type I allergy.


Subject(s)
Allergens/administration & dosage , Hypersensitivity, Immediate/prevention & control , Plant Proteins/administration & dosage , Pollen/immunology , Administration, Intranasal , Adoptive Transfer , Allergens/genetics , Allergens/immunology , Animals , Antigens, Plant , Betula/genetics , Betula/immunology , Cytokines/biosynthesis , Desensitization, Immunologic , Female , Hypersensitivity, Immediate/genetics , Hypersensitivity, Immediate/immunology , Immune Tolerance , Immunity, Mucosal , Immunoglobulin E/biosynthesis , Immunoglobulin G/biosynthesis , In Vitro Techniques , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Plant Proteins/genetics , Plant Proteins/immunology , Pollen/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , Recombinant Proteins/administration & dosage , Recombinant Proteins/genetics , Recombinant Proteins/immunology , T-Lymphocytes/immunology , Transforming Growth Factor beta/genetics
2.
Int Arch Allergy Immunol ; 124(1-3): 391-4, 2001.
Article in English | MEDLINE | ID: mdl-11307025

ABSTRACT

Type I allergy, frequently elicited by airborne allergens, has constantly increased within recent years. Birch pollen and its major allergen Bet v 1 represent a major source of type I allergens. By genetic engineering hypoallergenic Bet v 1 fragments were produced, which lost the IgE binding capacity but retained the T cell epitopes. We have established a murine model of aerosol sensitization to birch pollen and its major allergen Bet v 1, leading to type I allergic immune responses and airway hyperresponsiveness. In the present study we demonstrate that mucosal administration of recombinant Bet v 1 prior to sensitization led to allergen-specific suppression of B and T cell responses in vivo and in vitro, reduction of eosinophilic infiltration in the lungs and inhibition of airway hyperresponsiveness. Intranasal pretreatment with the nonanaphylactic fragments of Bet v 1 prevented allergic immune responses and airway inflammation to the same degree as the pretreatment with the complete molecule. We conclude from our studies that mucosal tolerance induction with hypoallergenic molecules could provide a safe and convenient treatment strategy against type I allergies.


Subject(s)
Asthma/immunology , Hypersensitivity, Immediate/immunology , Immune Tolerance , Immunity, Mucosal , Allergens/immunology , Animals , Antigens, Plant , Bronchial Hyperreactivity/immunology , Desensitization, Immunologic , Female , Immunoglobulin E/biosynthesis , Immunoglobulin G/biosynthesis , Mice , Mice, Inbred BALB C , Peptide Fragments/immunology , Plant Proteins/immunology , Pollen/immunology
3.
Am J Physiol Lung Cell Mol Physiol ; 280(3): L565-73, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11159041

ABSTRACT

A method for the noninvasive measurement of airway responsiveness was validated in allergic BALB/c mice. With head-out body plethysmography and the decrease in tidal midexpiratory flow (EF(50)) as an indicator of airway obstruction, responses to inhaled methacholine (MCh) and the allergen ovalbumin were measured in conscious mice. Allergen-sensitized and -challenged mice developed airway hyperresponsiveness as measured by EF(50) to aerosolized MCh compared with that in control animals. This response was associated with increased allergen-specific IgE and IgG1 production, increased levels of interleukin-4 and interleukin-5 in bronchoalveolar lavage fluid and eosinophilic lung inflammation. Ovalbumin aerosol challenge elicited no acute bronchoconstriction but resulted in a significant decline in EF(50) baseline values 24 h after challenge in allergic mice. The decline in EF(50) to MCh challenge correlated closely with simultaneous decreases in pulmonary conductance and dynamic compliance. The decrease in EF(50) was partly inhibited by pretreatment with the inhaled beta(2)-agonist salbutamol. We conclude that measurement of EF(50) to inhaled bronchoconstrictors by head-out body plethysmography is a valid measure of airway hyperresponsiveness in mice.


Subject(s)
Bronchial Hyperreactivity/etiology , Bronchial Hyperreactivity/physiopathology , Hypersensitivity/complications , Maximal Midexpiratory Flow Rate , Tidal Volume , Administration, Inhalation , Adrenergic beta-Agonists/pharmacology , Albuterol/pharmacology , Allergens/immunology , Animals , Bronchial Hyperreactivity/chemically induced , Bronchitis/physiopathology , Bronchoalveolar Lavage Fluid/chemistry , Bronchoconstrictor Agents , Cytokines/analysis , Female , Hypersensitivity/immunology , Immunoglobulins/blood , Lung Compliance , Methacholine Chloride , Mice , Mice, Inbred BALB C , Plethysmography/methods , Pulmonary Gas Exchange , Respiration , Time Factors
4.
Am J Respir Cell Mol Biol ; 23(2): 228-33, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10919990

ABSTRACT

The effects of repeated ozone exposures on the development of immune responses toward ovalbumin (OVA) were investigated in BALB/c and C57BL/6 mice. Ozone exposures (180 to 500 microg/m(3); 4 h, three times/wk for 4 wk) were combined with a protocol of OVA-aerosol exposure (1% OVA). Immediate cutaneous hypersensitivity (ICHS) reactions and antibody titers were assessed in parallel to cytokine levels of bronchoalveolar lavage fluids. In BALB/c mice, ozone triggered a T-helper (Th)2-like response indicated by dose-dependent increases in total serum immunoglobulin (Ig) E (from 133 to 821 ng/ml), interleukin (IL)-4 (from 60 to 208 pg/ml), and IL-5 levels (from 43 to 356 pg/ml), and by the recruitment of eosinophils and lymphocytes into the airways. Ozone exposure (500 microg/m(3)) in parallel to OVA-aerosol exposure increased anti-OVA IgG(1) antibody titers by 80%, leukotrienes (C(4)/D(4)/E(4)) by 60%, and airway responsiveness (11.3 versus 7.2 mg/ml methacholine), and doubled the frequency of positive ICHS reactions. In C57BL/6 mice, only the combination of OVA and ozone exposure induced positive ICHS reactions, doubled anti-OVA IgG(1), and suppressed anti-OVA IgG(2a) (-64%) antibody titers. Ozone, therefore, shifted the immune responses to OVA toward a Th2-like pattern in both "IgE-high responder" (BALB/c) and "IgE-low responder" (C57BL/6) mice.


Subject(s)
Hypersensitivity, Immediate/immunology , Oxidants, Photochemical/pharmacology , Ozone/pharmacology , Administration, Inhalation , Animals , Bronchial Hyperreactivity/immunology , Bronchoalveolar Lavage Fluid/cytology , Cell Count/drug effects , Dose-Response Relationship, Drug , Female , Immunoglobulin E/blood , Immunoglobulin E/drug effects , Immunoglobulin G/blood , Immunoglobulin G/drug effects , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Ovalbumin/immunology , Ovalbumin/pharmacology , Th2 Cells/cytology , Th2 Cells/drug effects , Th2 Cells/immunology
5.
Int Arch Allergy Immunol ; 121(1): 57-67, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10686510

ABSTRACT

BACKGROUND: Mouse models have been established mirroring key features of human bronchial asthma including airway hyperresponsiveness (AHR). Acute airway obstruction in response to an allergen challenge, however, remains to be demonstrated in these models. OBJECTIVE: A mouse model of allergic lung inflammation was employed to analyze the development of specific (allergen-induced) and nonspecific (methacholine-induced) airway obstruction. METHODS: Mice were sensitized to ovalbumin (OVA) and challenged with OVA aerosol twice each week during four weeks. Changes in lung functions were determined by noninvasive head-out body plethysmography. The development of acute airway obstruction after OVA challenge and AHR after methacholine aerosol application were assessed by a decrease in the mid-expiratory flow rate (EF(50)). RESULTS: Two airway challenges were sufficient to induce AHR (5.7 vs. 15 mg/ml methacholine). Further OVA challenges reduced the baseline EF(50) from 1.85 to 1.20 ml/s (4th week) and induced acute airway obstruction. The OVA-induced obstruction was maximal in the 4th week (EF(50) = 0.91 ml/s). CONCLUSION: The development of acute airway obstruction in allergen-sensitized mice was demonstrated by means of head-out body plethysmography. In our model, AHR was observed before the development of airway obstruction.


Subject(s)
Airway Obstruction/physiopathology , Asthma/physiopathology , Bronchial Hyperreactivity/physiopathology , Airway Obstruction/chemically induced , Airway Obstruction/immunology , Airway Resistance , Allergens/immunology , Animals , Asthma/chemically induced , Asthma/immunology , Bronchial Hyperreactivity/chemically induced , Bronchial Hyperreactivity/immunology , Bronchial Provocation Tests , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Immunoglobulin E/analysis , Immunoglobulin G/analysis , Maximal Expiratory Flow Rate , Methacholine Chloride , Mice , Mice, Inbred BALB C , Ovalbumin/immunology , Plethysmography, Whole Body , Tidal Volume
6.
Am J Respir Cell Mol Biol ; 21(4): 537-46, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10502564

ABSTRACT

The aim of this study was to investigate production and cellular sources of brain-derived neurotrophic factor (BDNF) production in allergic asthma. For this purpose a mouse model of chronic and severe ovalbumin (OVA)-induced airway inflammation was developed. Allergen-exposed mice developed elevated immunoglobulin E titers; airway inflammation with influx of lymphocytes, monocytes, and eosinophils; and airway hyperresponsiveness. In addition to an influx of inflammatory cells, interleukin (IL)-4 and IL-5 production were enhanced, macrophages showed morphologic signs of activation, and airway epithelium was thickened and displayed a goblet-cell hyperplasia with a marked mucus production. BDNF was detected using in situ hybridization and enzyme-linked immunosorbent assay. Constitutive expression of BDNF messenger RNA (mRNA) was observed in the respiratory epithelium of sensitized and nonsensitized mouse lungs. In addition, BDNF mRNA was detected in airway inflammatory infiltrations and bronchoalveolar lavage fluid (BALF) cells of OVA-sensitized and aerosol-challenged mice. Highest BDNF protein levels were detected in BALF after long-term allergen aerosol exposure. Analysis of BDNF production by isolated lymphocyte subsets revealed T but not B cells as a cellular source of BDNF. In addition, activated alveolar macrophages were identified as BDNF-positive cells. These data indicate that in allergic airway inflammation BDNF production is upregulated and immune cells serve as a source of BDNF.


Subject(s)
Asthma/etiology , Brain-Derived Neurotrophic Factor/biosynthesis , Allergens/administration & dosage , Animals , Asthma/immunology , Asthma/pathology , Brain-Derived Neurotrophic Factor/genetics , Bronchial Hyperreactivity/etiology , Bronchial Hyperreactivity/immunology , Disease Models, Animal , Humans , In Situ Hybridization , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Ovalbumin/immunology , RNA, Messenger/genetics , RNA, Messenger/metabolism
7.
Eur J Immunol ; 29(3): 1021-31, 1999 03.
Article in English | MEDLINE | ID: mdl-10092107

ABSTRACT

Although immunological consequences of systemic superantigen administration have been extensively studied, the effects of local mucosal exposure to superantigens are not well defined. The purpose of this study was to delineate the type of immune response triggered by superantigen exposure to the airway mucosa in mice. In dose-response experiments we determined a low dose of staphylococcal enterotoxin B (SEB) that triggered an inflammatory response characterized by mucosal and airway recruitment of lymphocytes, eosinophils and neutrophils together with elevated levels of IL-4, but not IFN-gamma, in bronchoalveolar lavage (BAL) fluids. TCR Vbeta analysis revealed that superantigen-responsive and -non-responsive T cells were equally recruited into the airways. SEB markedly enhanced the frequency of TNF-alpha-positive BAL macrophages as well as the amount of TNF-alpha in BAL fluids. These responses were associated with the development of increased airway responsiveness (AR) in SEB-treated mice. This effect occurred in an antibody-independent fashion. Furthermore, this type of response was observed in IgE-high responder BALB/c as well as in IgE-low/intermediate responder C57BL/6 mice. The development of increased AR was CD4+ T cell dependent as shown by transfer experiments into BALB/c nu/nu mice. These results suggest that the local immune response following mucosal superantigen administration triggers a unique inflammatory response in the airways resembling many features of "intrinsic asthma".


Subject(s)
Asthma/immunology , CD4-Positive T-Lymphocytes/immunology , Enterotoxins/immunology , Lung/immunology , Models, Immunological , Staphylococcus aureus/immunology , Superantigens/immunology , Administration, Intranasal , Animals , Bronchoalveolar Lavage Fluid/cytology , Bronchoalveolar Lavage Fluid/immunology , Enterotoxins/administration & dosage , Immunoglobulin Variable Region/immunology , Interleukin-4/biosynthesis , Macrophages, Alveolar/immunology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Receptors, Antigen, T-Cell, alpha-beta/immunology , Superantigens/administration & dosage , Tumor Necrosis Factor-alpha/biosynthesis
8.
Toxicology ; 123(3): 185-95, 1997 Dec 05.
Article in English | MEDLINE | ID: mdl-9355937

ABSTRACT

Seven agents were analyzed with respect to their ability to induce heat shock protein (HSP) synthesis in C6 rat glioma cells. Induction of HSP synthesis was correlated with cytotoxicity and lipophilicity of the substances. In addition to the first four n-alcohols (methanol, ethanol, propanol and butanol) and phenol, whose capacity to induce HSP was analyzed earlier (Neuhaus-Steinmetz et al., 1994. Mol. Pharmacol. 45, 36-41), isopropanol, 1,4-dinitrophenol (DNP), diethylstilbestrol (DES), carbonylcyanide-m-chlorophenylhydrazone (CCCP), rotenone, paracetamol and acetyl salicylic acid (ASA) induced HSP synthesis after a 1-h incubation at a substance-specific concentration. The maximal induction of HSPs was closely correlated with the cytotoxicity of all substances and occurred when cell viability was reduced to 75 +/- 11% of the controls. Cytotoxicity and the ability to induce HSP were correlated with the lipophilicity of the alcohols, phenol, rotenone and paracetamol. Calculation of the hypothetical membrane concentrations of these compounds yielded a nearly equal value (0.54 +/- 0.13 M), indicating that interaction of substances with lipophilic cellular compounds, such as membranes or lipophilic core regions of proteins, is a critical step leading to HSP induction. This assumption is supported by a correlation between HSP induction and protein denaturation by the different alcohols (Herskovits et al., 1970. J. Biol. Chem. 245, 2588-2598). We assume that the amount of misfolded proteins induced by these lipophilic agents is responsible for the induction of HSP synthesis. ASA, DNP and CCCP induced HSP at lower concentrations than substances with a similar lipophilicity, which may be due to effects which add to the misfolding of proteins or to other signal pathways.


Subject(s)
Cell Survival/drug effects , Glioma/metabolism , Heat-Shock Proteins/biosynthesis , Lipid Metabolism , Pharmaceutical Preparations , Protein Denaturation/drug effects , Tumor Cells, Cultured/metabolism , Actins/metabolism , Animals , Autoradiography , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Glioma/drug therapy , Rats , Tumor Cells, Cultured/drug effects
9.
Brain Res ; 746(1-2): 220-30, 1997 Jan 23.
Article in English | MEDLINE | ID: mdl-9037501

ABSTRACT

The effects of two different stressors, heat shock (HS; 44 degrees C, 20 min) and ethanol (1.2 M, 60 min), on ion content and membrane potential were investigated in C6 rat glioma cells. Both treatments were previously shown to induce the HS response [26]. Intracellular pH (pH(i)), sodium ion concentration ([NA+]i), potassium ion concentration ([K+]i) and membrane potential were determined by means of continuous 31P and 23Na nuclear magnetic resonance (NMR), continuous fluorescence spectroscopy and 86Rb uptake. Lactate extrusion was determined in addition with respect to pH(i) regulation. The aim of this study was a detailed picture of HS and ethanol-induced ion changes in a single cell type, because stress-induced changes in the intracellular ionic balance may be important factors for determining proliferation, stress response and apoptosis. HS lowered the pH(i) from 7.38 +/- 0.04 to about 7.05 +/- 0.04. [Na+]i decreased during HS to 50% of the control and recovered to normal level 95 min after HS treatment. During HS, [K+]i remained constant but increased after HS. The membrane potential hyperpolarized from -83 mV to -125 mV and returned to initial values during HS treatment. Lactate extrusion increased 3-fold after HS. Ethanol (1.2 M) lowered the pH(i) from pH 7.38 +/- 0.04 to pH 7.0 +/- 0.04, but in contrast to heat strongly increased [Na]i. It hyperpolarized the membrane potential from -83 to -125 mV. Ethanol also increased lactate extrusion similar to HS. Also in contrast to the effect of HS, the potassium concentration decreased during ethanol treatment. The Na(+)-H+ exchanger monensin was used to overcome the apparent inhibition of the cellular Na(+)-H+ exchanger by HS. At normal pH(e) (7.4) monensin increased [Na+]i and pH(i) considerably. A subsequent HS reduced [Na+]i only minimally. Acidification of the cells by low pH(e) (6.2) prior to HS did not abolish the HS-induced drop of pH(i), indicating that the Na(+)-H+ exchanger was also inhibited at low pH(i). At low pH(e), monensin transports H+ into the cell. A subsequent HS decreased pH(i) only little, showing the importance of inhibition of the Na(+)-H+ exchanger for the HS-induced pH(i) decrease. 100 microM amiloride reduced pH(i) and [Na+]i in a similar way as HS, but did not change pH(i) and [Na+]i much during a HS. These results indicate that some of the HS-induced ionic changes are mediated by inhibition of the Na(+)-H+ exchanger, activation of Na(+)-K(+)-ATPase and changes of membrane conductance for ions.


Subject(s)
Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Glioma , Animals , Biological Transport/physiology , Hot Temperature , Hydrogen-Ion Concentration , Ionophores/pharmacology , Kinetics , Lactates/metabolism , Magnetic Resonance Spectroscopy , Membrane Potentials/drug effects , Monensin/pharmacology , Potassium/metabolism , Protons , Rats , Sodium/metabolism , Sodium-Hydrogen Exchangers/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Spectrometry, Fluorescence , Tumor Cells, Cultured/chemistry , Tumor Cells, Cultured/drug effects , Tumor Cells, Cultured/enzymology
10.
Brain Res ; 724(1): 16-24, 1996 Jun 10.
Article in English | MEDLINE | ID: mdl-8816251

ABSTRACT

The effect of intracellular pH (pHi) on heat shock protein (HSP) synthesis was investigated in C6 rat glioma cells. pHi changes were analysed by means of fluorescence spectroscopy in a perfused monitoring system allowing continuous measurements before, during and after treatments. HSP induction was determined by means of Western blots and autoradiographs. A 20 min heat shock (HS) of 44 degrees C decreased the pHi from 7.36 to 7.05 during exposure [17] and elicited the synthesis of heat shock proteins 2-8 h later. A pHi decrease, brought about by low extracellular pH (pHe) of 4.5 and 5.0 or 5.5, induced HSP synthesis after 1 h or 3 h, respectively. During these treatments, pHi decreased to values significantly lower than that caused by HS. Three h exposure to pHe 6.2, however, was not inductive. These results indicate that the heat shock-induced pHi decrease alone is not sufficient to stimulate HSP synthesis. In order to investigate the effect of alkaline pHi on the induction of HSP by heat, pHi was increased prior to HS treatments. Preincubation of cells at pHe ranging from 6.8 to 8.0 had little effect on pHi and on HSP synthesis. A shift of pHi to more alkaline values was achieved by adding the H+/Na+ exchanger monensin at alkaline pHe. Twenty microM monensin raised the pHi and inhibited the HSP induction depending on the pHe values: as pHe was increased from pH 7.2 to 8.0 HSP synthesis was increasingly inhibited. Monensin also diminished the HS-induced drop of pHi particularly at higher pHe. The result showed that neither a lower pHi nor a drop of pHi during HS is a necessary prerequisite for the induction, whereas alkalosis inhibits the synthesis of HSP.


Subject(s)
Alkalosis/metabolism , Heat-Shock Proteins/metabolism , Alkalosis/chemically induced , Animals , Autoradiography , Blotting, Western , Fluorescent Dyes , Glioma , Hydrogen-Ion Concentration , Monensin/adverse effects , Neutral Red , Rats , Tumor Cells, Cultured/metabolism
11.
Mol Pharmacol ; 45(1): 36-41, 1994 Jan.
Article in English | MEDLINE | ID: mdl-8302278

ABSTRACT

In C6 rat glioma cells, the n-alcohols methanol, ethanol, propanol, and butanol and the aromatic alcohol phenol all induce heat shock proteins (HSPs) of high molecular mass (68, 70, 90, and 110 kDa) when applied for 1 hr. The lowest alcohol concentrations that induce HSP synthesis cause about 20% cell death, as determined by neutral red assay. HSP induction thus occurs at alcohol concentrations close to the highest tolerable dose. The cytotoxicity and the potential of alcohols to induce the synthesis of HSPs increase with chain length and are correlated with the lipophilicity of the alcohols. A clear structure-activity relationship is observed for both parameters. A calculation of the putative membrane concentrations of these alcohols reveals that cytotoxic effects (50% cell death) occur at nearly the same membrane concentration (approximately 0.2 M). This also holds true for the lowest HSP 68-inducing alcohol concentrations, but at a lower concentration (approximately 0.12 M). The activities of major proteinases are affected by both heat shock and alcohols. The effects of alcohols also depend on the lipophilicity of the alcohols. Effective concentrations again are close to the highest tolerable dose. The stress reactions measured in terms of significant changes in HSP synthesis and proteinase activity provide information about the mechanisms by which toxic agents act on the cell.


Subject(s)
Alcohols/pharmacology , Heat-Shock Proteins/biosynthesis , Alcohols/chemistry , Alcohols/toxicity , Animals , Blotting, Western , Cell Survival , Endopeptidases/metabolism , Hot Temperature , Neutral Red , Rats , Structure-Activity Relationship , Trypan Blue , Tumor Cells, Cultured
12.
Experientia ; 45(5): 476-7, 1989 May 15.
Article in English | MEDLINE | ID: mdl-2566511

ABSTRACT

Tyrosine aminotransferase activity of Euglena oscillates with an ultradian period of approximately 4-5 h. The oscillation frequency in the time series was determined by cosine fitting. Experiments which were performed between 16 and 31.5 degrees C revealed temperature compensation.


Subject(s)
Activity Cycles , Circadian Rhythm , Euglena gracilis/physiology , Tyrosine Transaminase/metabolism , Animals , Euglena gracilis/enzymology , Temperature
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