ABSTRACT
The aim of this study was to evaluate and compare the mitogenic effect of peritoneal fluid (PF) from women with mild and severe endometriosis on the endometrial stromal cell proliferation. Increasing concentrations of PF from women with and without mild or severe endometriosis were added to primary endometrial stromal cell cultures and 3H-thymidine incorporation was used to assess DNA synthesis in these cultures. PF from women with mild endometriosis induced a statistically significant dose-dependent increase in stromal cell thymidine uptake ranged from 5.8 to 14.5 fold, whereas PF from women with severe endometriosis produced an average 51% inhibition of stromal cell proliferation of compared with cells exposed to non-endometriosis PF or exposed to nutrient medium supplemented with 2.5% calf serum alone. PF samples from patients with stage I endometriosis induced a statistically dose-dependent increase in stromal cell proliferation, whereas PF from patients with stage IV endometriosis caused a significant inhibition.
Subject(s)
Ascitic Fluid/physiopathology , Endometriosis/physiopathology , Endometrium/cytology , Cell Division/physiology , Cells, Cultured , DNA/biosynthesis , Female , Humans , Thymidine/metabolismABSTRACT
Three patients with primary sterility in whom the majority of spermatozoa lacked a normally implanted head are presented. A small cephalic knob was evident in most of them by routine colorimetric techniques, and the Feulgen reaction failed to show any deoxyribose nucleic acid. The morphologic features of the tails was normal. Few loose sperm heads were observed in the ejaculates. Even though motility was decreased, there were numerous acephalic sperms with different degrees of forward motility. Electron microscopy showed a well-organized structure of the centrioles and connecting piece, which were located in the neck region within a small cytoplasmic mass, but no chromatin was detected in any case. Studies on immature spermatids present in semen evidenced an independent anomalous development of heads and tails and suggested that they became separated at the end of spermatid maturation. This anomaly, of probable genetic origin, is interpreted to be due either to an alteration in the mechanism of migration and positioning of the tail on the caudal pole of the nucleus or to an interference with the formation of the implantation fossa of the head, which normally accommodates the connecting piece.
Subject(s)
Infertility, Male/diagnosis , Sperm Head/abnormalities , Spermatozoa/abnormalities , Adult , Cytological Techniques , Fertilization , Humans , Male , Microscopy, Electron , Sperm Head/ultrastructure , Sperm Motility , Sperm Tail/ultrastructure , SyndromeABSTRACT
Con el objeto de evaluar el cuerpo amarillo como elemento en el estudio dinÔmico del ciclo ovulatorio, se investigó su morfología en 45 pacientes, separadas en dos grupos, uno con disfunción de la fase lútea y otro con ciclos ovulatorios normales. Se correlacionó el crecimiento del cuerpo amarillo con el endometrio y con el momento ovulatorio determinado por el pico de LH y/o TB. A través de los parámetros analizados se concluyó: 1) Que la técnica de la biopsia del cuerpo amarillo no es compleja ni ha presentado complicaciones a través de nuestra experiencia; 2) Que la biopsia del cuerpo amarillo es útil en la investigación de la fisiopatología de la fase lútea; 3) Que en los ciclos ovulatorios normales, la biopsia del cuerpo amarillo aporta datos de valor al estudio del endometrio de la implantación (AU)
Subject(s)
Humans , Female , Corpus Luteum/cytology , Ovulation DetectionABSTRACT
Con el objeto de evaluar el cuerpo amarillo como elemento en el estudio dinâmico del ciclo ovulatorio, se investigó su morfología en 45 pacientes, separadas en dos grupos, uno con disfunción de la fase lútea y otro con ciclos ovulatorios normales. Se correlacionó el crecimiento del cuerpo amarillo con el endometrio y con el momento ovulatorio determinado por el pico de LH y/o TB. A través de los parámetros analizados se concluyó: 1) Que la técnica de la biopsia del cuerpo amarillo no es compleja ni ha presentado complicaciones a través de nuestra experiencia; 2) Que la biopsia del cuerpo amarillo es útil en la investigación de la fisiopatología de la fase lútea; 3) Que en los ciclos ovulatorios normales, la biopsia del cuerpo amarillo aporta datos de valor al estudio del endometrio de la implantación
