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1.
Rev. bras. ativ. fís. saúde ; 29: 1-15, abr. 2024. fig, tab, quad
Article in Portuguese | LILACS | ID: biblio-1561336

ABSTRACT

Identificar e analisar os temas presentes em artigos que relacionam, de alguma maneira, a Educação Física e o Programa Academia da Saúde é o objetivo deste manuscrito. Os dados foram coletados nos bancos de dados LILACS, SciELO, Biblioteca Virtual em Saúde, Portal dos Periódicos Eletrônicos e em 12 periódicos da Educação Física. Foram selecionados e analisados os conteúdos de 54 artigos. Os resultados mostraram que houve maior número de publicações em 2020, com destaque para pesquisas realizadas no estado de Minas Gerais. O maior número de publicações consta na Revista Brasileira de Atividade Física & Saúde. Detecta-se o interesse crescente por estudos com objetos ampliados que considerem a complexidade das condições de saúde dos grupos e das comunidades atendidas no programa, especialmente a partir das aproximações com a Saúde Coletiva.


The aim of this manuscript is to identify and analyze the themes present in articles that in some way relate Physical Education and the the Health Gym Program, of Brazil. Data was collected from the LILACS, SciELO, Virtual Health Library, Electronic Periodicals Portal and 12 Physical Education journals. The contents of 54 articles were selected and analyzed. The results showed that there was a greater number of publications in 2020, with an emphasis on research carried out in the state of Minas Gerais. The largest number of publications was in the Brazilian Journal of Physical Activity & Health. There is a growing interest in studies with broader objects that consider the complexity of the health conditions of the groups and communities served by the program, especially based on approaches to Collective Health.


Subject(s)
Physical Education and Training , Exercise , Public Health
2.
Comput Biol Med ; 170: 108052, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38308868

ABSTRACT

The imbalance of epigenetic regulatory mechanisms such as DNA methylation, which can promote aberrant gene expression profiles without affecting the DNA sequence, may cause the deregulation of signaling, regulatory, and metabolic processes, contributing to a cancerous phenotype. Since some metabolites are substrates and cofactors of epigenetic regulators, their availability can be affected by characteristic cancer cell metabolic shifts, feeding cancer onset and progression through epigenetic deregulation. Hence, there is a need to study the influence of cancer metabolic reprogramming in DNA methylation to design new effective treatments. In this study, a generic Genome-Scale Metabolic Model (GSMM) of a human cell, integrating DNA methylation or demethylation reactions, was obtained and used for the reconstruction of Genome-Scale Metabolic Models enhanced with Enzymatic Constraints using Kinetic and Omics data (GECKOs) of 31 cancer cell lines. Furthermore, cell-line-specific DNA methylation levels were included in the models, as coefficients of a DNA composition pseudo-reaction, to depict the influence of metabolism over global DNA methylation in each of the cancer cell lines. Flux simulations demonstrated the ability of these models to provide simulated fluxes of exchange reactions similar to the equivalent experimentally measured uptake/secretion rates and to make good functional predictions. In addition, simulations found metabolic pathways, reactions and enzymes directly or inversely associated with the gene promoter methylation. Two potential candidates for targeted cancer epigenetic therapy were identified.


Subject(s)
DNA Methylation , Neoplasms , Humans , DNA Methylation/genetics , Epigenesis, Genetic , Cell Line , Neoplasms/genetics , Genome
3.
Bioact Mater ; 34: 311-325, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38274293

ABSTRACT

More effective approaches are needed in the treatment of blood cancers, in particular acute myeloid leukemia (AML), that are able to eliminate resistant leukemia stem cells (LSCs) at the bone marrow (BM), after a chemotherapy session, and then enhance hematopoietic stem cell (HSC) engraftment for the re-establishment of the HSC compartment. Here, we investigate whether light-activatable nanoparticles (NPs) encapsulating all-trans-retinoic acid (RA+NPs) could solve both problems. Our in vitro results show that mouse AML cells transfected with RA+NPs differentiate towards antitumoral M1 macrophages through RIG.1 and OASL gene expression. Our in vivo results further show that mouse AML cells transfected with RA+NPs home at the BM after transplantation in an AML mouse model. The photo-disassembly of the NPs within the grafted cells by a blue laser enables their differentiation towards a macrophage lineage. This macrophage activation seems to have systemic anti-leukemic effect within the BM, with a significant reduction of leukemic cells in all BM compartments, of animals treated with RA+NPs, when compared with animals treated with empty NPs. In a separate group of experiments, we show for the first time that normal HSCs transfected with RA+NPs show superior engraftment at the BM niche than cells without treatment or treated with empty NPs. This is the first time that the activity of RA is tested in terms of long-term hematopoietic reconstitution after transplant using an in situ activation approach without any exogenous priming or genetic conditioning of the transplanted cells. Overall, the approach documented here has the potential to improve consolidation therapy in AML since it allows a dual intervention in the BM niche: to tackle resistant leukemia and improve HSC engraftment at the same time.

4.
Article in English | MEDLINE | ID: mdl-37925270

ABSTRACT

OBJECTIVE: Sirolimus (SRL) and everolimus (EVL) are increasingly included in immunosuppressive protocols after heart transplantation. They present some side effects, including the appearance of painful lesions in the oral cavity. Therefore, this systematic review aimed to verify the global prevalence and clinical characteristics of oral lesions induced by SRL and EVL in heart transplant patients. STUDY DESIGN: A systematic review was performed using 5 main electronic databases (Medline/PubMed, SCOPUS, EMBASE, Web of Science, and LILACS), in addition to the gray literature. Studies were independently assessed by 2 reviewers based on established eligibility criteria. The risk of bias was assessed using the Joanna Briggs Institute appraisal tools, and the certainty of evidence was evaluated through GRADE assessment. RESULTS: Seventeen studies (860 patients) were included in the qualitative analysis. Of these, 11 studies were pooled in a meta-analysis of prevalence. The worldwide prevalence of oral lesions induced by SRL and EVL in heart transplant patients was 10.0%, and most lesions were described as ulcers >1.0 cm, related to significant pain. CONCLUSIONS: Oral lesions induced by SRL and/or EVL, although not very prevalent, have a relevant impact on patient's lives and the continuity of treatment.


Subject(s)
Everolimus , Heart Transplantation , Humans , Everolimus/adverse effects , Sirolimus/adverse effects , Prevalence , Immunosuppressive Agents/adverse effects , Heart Transplantation/adverse effects
5.
J Evid Based Dent Pract ; 23(3): 101863, 2023 09.
Article in English | MEDLINE | ID: mdl-37689449

ABSTRACT

BACKGROUND: Dual antiplatelet therapy (DAPT) provides additional risk reduction of ischemic events compared to aspirin monotherapy, at cost of higher bleeding risk. There are few data comparing new techniques for reducing bleeding after dental extractions in these patients. PURPOSE: This study investigated the effectiveness of the HemCon Dental Dressing (HDD) compared to oxidized cellulose gauze. MATERIALS AND METHODS: This randomized study included 60 patients on DAPT who required at least two dental extractions (120 procedures). Each surgical site was randomized to HDD or oxidized regenerated cellulose gauze as the local hemostatic method. Intra-oral bleeding time was measured immediately after the dental extraction and represents our main endpoint for comparison of both hemostatic agents. Prolonged bleeding, platelet reactivity measured by Multiplate Analyser (ADPtest and ASPItest) and tissue healing comparison after 7 days were also investigated. RESULTS: Intra-oral bleeding time was lower in HDD compared with control (2 [2-5] vs. 5 [2-8] minutes, P=0.001). Prolonged postoperative bleeding was observed in 7 cases (11.6%), all of them successfully managed with local sterile gauze pressure. More HDD treated sites presented better healing when compared with control sites [21 (36.8%) vs. 5 (8.8%), P=0.03]. There was poor correlation between platelet reactivity and intra-oral bleeding time. CONCLUSIONS: In patients on DAPT, HDD resulted in a lower intra-oral bleeding time compared to oxidized cellulose gauze after dental extractions. Moreover, HDD also seems to improve healing conditions.


Subject(s)
Cellulose, Oxidized , Hemostatics , Humans , Cellulose, Oxidized/therapeutic use , Platelet Aggregation Inhibitors/therapeutic use , Single Person , Single-Blind Method , Hemostatics/therapeutic use , Tooth Extraction
6.
J Clin Rheumatol ; 29(7): 341-346, 2023 Oct 01.
Article in English | MEDLINE | ID: mdl-37748433

ABSTRACT

OBJECTIVE: The aim of this study was to analyze HLA alleles in patients with Behçet disease (BD) and their correlation with ophthalmic manifestations (OMs) in a multiethnic Brazilian population. METHODS: This case-control study compared 72 BD patients with or without OM who underwent a thorough ophthalmologic evaluation, including best-corrected visual acuity, bino-ophthalmoscopy, and HLA analysis, with 144 matched healthy controls. Fluorescein angiography was also performed in the patients with BD and OM. HLA class I (A, B, and C) and II (DRB1, DQB1, and DQA1) typing were performed using PCR-SSO. RESULTS: Of 72 patients with BD, 42 (58%) had OM. The HLA-B*51 and -A*26 alleles were more frequent in patients with BD than in controls (23.6% vs 14.6% and 12.5% vs 4.3%, respectively), but could not differentiate OM risk. The HLA alleles of BD patients that differentiated those with and without OM were HLA-B*15 (40.5% vs 20.7%; odds ratio [OR], 2.59; p = 0.0059), HLA-C*02 (33.3% vs 13.4%; OR, 3.20; p = 0.0024), and HLA-DQB1*03 (64.3% vs 45.7%, p = 0.017), whereas HLA-A*03 (0.0% vs 13.3%, p = 0.006) and HLA-DRB1*15 (4.8% vs 19.5%; OR, 0.21; p = 0.0121) were protective against OM. CONCLUSIONS: In this study of a Brazilian multiethnic BD population, alleles were similar between groups of BD patients with and without OM. We described HLA-B*15, -C*02, and -DQB1*03 as risk factors and -A*03 and -DRB1*15 as protective factors for OM in BD, which could function as biomarkers for predicting disease phenotypes.


Subject(s)
Behcet Syndrome , Humans , Behcet Syndrome/diagnosis , Behcet Syndrome/genetics , Alleles , Case-Control Studies , Risk Factors , Brazil/epidemiology
7.
J Indian Soc Periodontol ; 27(4): 392-398, 2023.
Article in English | MEDLINE | ID: mdl-37593548

ABSTRACT

Background: To analyze through finite element analysis the stress distribution in peri-implant bone tissues, implants, and prosthetic components induced by the socket shield (SS) technique in comparison to other techniques used to treat tooth loss. Materials and Methods: A three-dimensional model of a superior central incisor crown supported by implant was modeled and three different placement conditions were simulated: SS - 2.0-mm-thick root dentin fragment positioned between the alveolar buccal wall and implant; heterologous bone graft (HBG) - bovine bone graft positioned the alveolar buccal wall and implant; and control (C) - implant fully placed in bone tissue of a healed alveolus. The model was restricted at the lateral surfaces of the bone tissue and the following loads were simulated: Both oblique (45°) loads of 100 N on the lingual surface of the crown (maximal habitual intercuspation) and 25.5 N on the incisal edge of the crown (tooth contact during mandibular protrusion) were simultaneously applied. Tensile stress, shear stress, compression, and displacement were analyzed in the cortical bone, trabecular bone, dentin root fragment, and bone graft; while equivalent von Mises stresses were quantified in the implant and prosthetic components. Results: Stress values of SS and HBG in the bone tissues were higher than C, while slight differences within models were observed for dentin root fragment, bone graft, implant, and prosthetic components. Conclusions: The SS technique presented the highest stress concentration in the peri-implant tissues.

8.
Antioxidants (Basel) ; 12(6)2023 Jun 14.
Article in English | MEDLINE | ID: mdl-37372004

ABSTRACT

Signaling and detoxification of Reactive Oxygen Species (ROS) are important patho-physiologcal processes. Despite this, we lack comprehensive information on individual cells and cellular structures and functions affected by ROS, which is essential to build quantitative models of the effects of ROS. The thiol groups from cysteines (Cys) in proteins play a major role in redox defense, signaling, and protein function. In this study, we show that the proteins in each subcellular compartment contain a characteristic Cys amount. Using a fluorescent assay for -SH in thiolate form and amino groups in proteins, we show that the thiolate content correlates with ROS sensitivity and signaling properties of each compartment. The highest absolute thiolate concentration was found in the nucleolus, followed by the nucleoplasm and cytoplasm whereas protein thiolate groups per protein showed an inverse pattern. In the nucleoplasm, protein reactive thiols concentrated in SC35 speckles, SMN, and the IBODY that accumulated oxidized RNA. Our findings have important functional consequences, and explain differential sensitivity to ROS.

9.
Article in Portuguese | LILACS | ID: biblio-1537176

ABSTRACT

Esta revisão narrativa buscou descrever e caracterizar os conceitos de corpo operados no campo da Educação Física brasileira. O material explorado indicou que os conceitos são apropriados de obras clássicas, ramificam-se e representam abordagens teóricas distintas que influenciam os conhecimentos, saberes e práticas. Os conceitos demonstram as divergências e contradições entre a dimensão natural e social, assim como a hegemonia do modelo biomédico no campo. O conceito de práticas corporais tem sido basilar para construir intervenções humanizadas e críticas com sujeitos e comunidades, considerando suas necessidades de saúde (AU).


This narrative review aimed to describe and characterize the concepts of body operated in the field of Brazilian Physical Education. The material explored indicated that the concepts are appropriated from classical works, branch out and represent divergent theoretical approaches that influence knowledge, know-how, and practices. The concepts demonstrate the divergences and contradictions between the natural and social dimensions, as well as the hegemony of the biomedical model in the field. The concept of body practices has been fundamental to build humanized and critical interventions with subjects and communities considering their health needs (AU).


Esta revisión narrativa tuvo como objetivo describir y caracterizar los conceptos de cuerpo operado en el ámbito de la Educación Física brasileña. El material explorado indica que los conceptos se apropian de obras clásicas, se ramifican y representan enfoques teóricos divergentes que influyen en el saber, el saber hacer y las prácticas. Los conceptos demuestran las divergencias y contradicciones entre las dimensiones natural y social, así como la hegemonía del modelo biomédico en el campo. El concepto de prácticas corporales ha sido fundamental para construir intervenciones humanizadas y críticas con individuos y comunidades teniendo en cuenta sus necesidades sanitarias (AU).


Subject(s)
Humans , Exercise , Health-Disease Process , Human Body , Brazil
11.
Int. j. cardiovasc. sci. (Impr.) ; 36: e20200312, 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1430488

ABSTRACT

Abstract Background Dental anesthetic management in implantable cardioverter defibrillator (ICD) recipients with cardiac channelopathies (CCh) can be challenging due to the potential risk of life-threatening arrhythmias and appropriate ICD therapies during procedural time. Objectives The present study assessed the hypothesis that the use of local dental anesthesia with 2% lidocaine with 1:100,000 epinephrine or without a vasoconstrictor can be safe in selected ICD and CCh patients, not resulting in life-threatening events (LTE). Methods Restorative dental treatment under local dental anesthesia was made in two sessions, with a wash-out period of 7 days (cross-over trial), conducting with a 28h - Holter monitoring, and 12-lead electrocardiography, digital sphygmomanometry, and anxiety scale assessments in 3 time periods. Statistical analysis carried out the paired Student's t test and the Wilcoxon signed-rank test. In all cases, a significance level of 5% was adopted. All patients were in stable condition with no recent events before dental care. Results Twenty-four consecutive procedures were performed in 12 patients (9 women, 3 men) with CCh and ICD: 7 (58.3%) had long QT syndrome (LQTS), 4 (33.3%) Brugada syndrome (BrS), and 1 (8.3%) Catecholaminergic polymorphic ventricular tachycardia (CPVT). Holter analysis showed no increased heart rate (HR) or sustained arrhythmias. Blood pressure (BP), electrocardiographic changes and anxiety measurement showed no statistically significant differences. No LTE occurred during dental treatment, regardless of the type of anesthesia. Conclusion Lidocaine administration, with or without epinephrine, can be safely used in selected CCh-ICD patients without LTE. These preliminary findings need to be confirmed in a larger population with ICD and CCh.

12.
Motrivivência (Florianópolis) ; 35(66): 1-15, 2023.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1524085

ABSTRACT

O presente estudo trata da crise da razão objetiva, da formalização da razão subjetiva, passando pela crise da razão no ocidente e chegando à crise da racionalidade na Educação Física. Trabalhamos com a ideia de que a razão subjetiva se desenvolveu mediante progressos técnicos e científicos, e a razão objetiva mergulhou em uma narrativa que julga as ações dos sujeitos a partir das circunstâncias. A cada dia há alienação exacerbada e torna os seres humanos marionetes no sistema capitalista de produção de mercadoria. Nesse sentido, a Educação Física é apresentada como uma razão objetivista com fundamentos de uma razão subjetiva "sem", contudo, avançar para uma única matriz de referência epistemológica, mas que nem por isso devemos deixar de repensá-la por outros modos. Defendemos a cultura corporal como objeto de estudo do campo e a necessidade da manutenção das subáreas da biodinâmica, sociocultural e pedagógica para o desenvolvimento do campo, apesar das lutas e disputas em jogo nesse campo.


The present study deals with the crisis of objective reason, the formalization of subjective reason, going through the crisis of reason in the West and arriving at the crisis of rationality in Physical Education. We developed the idea that subjective reason was developed through technical and scientific progress, and objective reason was immersed in a narrative that judges the actions of subjects based on circumstances. Every day there is exacerbated alienation and makes human beings puppets within the capitalist system of commodity production. In this sense, Physical Education is presented as an objectivist reason based on a subjective reason "without", however, moving towards a single epistemological reference matrix, but that does not mean that we must rethink it in other ways. We defend body culture as an object of study in the field and the need to maintain the subareas of biodynamics, sociocultural and pedagogical for the development of the field, despite the struggles and disputes at stake in the field.


El presente estudio aborda la crisis de la razón objetiva, la formalización de la razón subjetiva, pasando por la crisis de la razón en Occidente y llegando a la crisis de la racionalidad en la Educación Física. Desarrollamos la idea de que la razón subjetiva se desarrolló a través del progreso técnico y científico, y la razón objetiva estaba inmersa en una narrativa que juzga las acciones de los sujetos en función de las circunstancias. Cada día se exacerba la alienación y se convierte a los seres humanos en marionetas dentro del sistema capitalista de producción de mercancías. En este sentido, la Educación Física se presenta como una razón objetivista basada en una razón subjetiva "sin", sin embargo, avanzar hacia una única matriz epistemológica de referencia, pero eso no significa que debamos repensarla de otras maneras. Defendemos la cultura corporal como objeto de estudio en el campo y la necesidad de mantener las subáreas de biodinámica, sociocultural y pedagógica para el desarrollo del campo, a pesar de las luchas y disputas en juego en el campo.

13.
PLoS One ; 17(10): e0276154, 2022.
Article in English | MEDLINE | ID: mdl-36228016

ABSTRACT

Several studies comparing resistance training (RT) frequencies may have been affected by the large between-subject variability. This study aimed to compare the changes in lower limbs maximal dynamic strength (1RM) and quadriceps femoris cross-sectional area (CSA) after a RT with different weekly frequencies in strength-trained individuals using a within-subject design. Twenty-four men participated in a 9-week RT program, being randomly divided into two conditions: resistance training with equalized total training volume (RTEV) and with unequalized total training volume (RTUV). The RT protocol used the unilateral leg press 45° exercise and each subject's lower limb executed one of the proposed frequencies (one and three times/week). All conditions effectively increased 1RM and CSA (p<0.001); however, no significant differences were observed in the values of 1RM (p = 0.454) and CSA (p = 0.310) between the RT frequencies in the RTEV and RTUV conditions. Therefore, RT performed three times a week showed similar increases in 1RM and CSA to the program performed once a week, regardless of training volume equalization. Nevertheless, when the higher RT frequency allowed the application of a greater TTV (i.e., RTUV), higher effect size (ES) values (0.51 and 0.63, 1RM and CSA, respectively) were observed for the adaptations.


Subject(s)
Muscle Strength , Resistance Training , Humans , Hypertrophy , Male , Muscle Strength/physiology , Muscle, Skeletal/physiology , Quadriceps Muscle/physiology , Resistance Training/methods , Weight Lifting
16.
Food Chem Toxicol ; 166: 113198, 2022 Aug.
Article in English | MEDLINE | ID: mdl-35671903

ABSTRACT

Amanita phalloides is one of the most toxic mushrooms worldwide, being responsible for the majority of human fatal cases of mushroom intoxications. α-Amanitin, the most deleterious toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and renal failure. Herein, we used cyclosporine A after it showed potential to displace RNAP II α-amanitin in silico. That potential was not confirmed either by the incorporation of ethynyl-UTP or by the monitoring of fluorescent RNAP II levels. Nevertheless, concomitant incubation of cyclosporine A with α-amanitin, for a short period, provided significant protection against its toxicity in differentiated HepaRG cells. In mice, the concomitant administration of α-amanitin [0.45 mg/kg intraperitoneal (i.p.)] with cyclosporine A (10 mg/kg i.p. plus 2 × 10 mg/kg cyclosporine A i.p. at 8 and 12 h post α-amanitin) resulted in the full survival of α-amanitin-intoxicated mice, up to 30 days after the toxin's administration. Since α-amanitin is a substrate of the organic-anion-transporting polypeptide 1B3 and cyclosporine A inhibits this transporter and is a potent anti-inflammatory agent, we hypothesize that these mechanisms are responsible for the protection observed. These results indicate a potential antidotal effect of cyclosporine A, and its safety profile advocates for its use at an early stage of α-amanitin intoxications.


Subject(s)
Alpha-Amanitin , Mushroom Poisoning , Alpha-Amanitin/metabolism , Alpha-Amanitin/toxicity , Amanita , Animals , Antidotes/pharmacology , Cyclosporine/toxicity , Humans , Liver , Mice
17.
Article in English | MEDLINE | ID: mdl-35640792

ABSTRACT

Subzero temperatures are among the most significant factors defining the distribution of organisms, yet, certain taxa have evolved to overcome this barrier. The microscopic tardigrades are among the most freeze-tolerant animals, with selected species reported to survive milli-Kelvin temperatures. Here, we estimate survival of fully hydrated eutardigrades of the species Ramazzottius varieornatus following exposures to -20 °C and  -80 °C as well as -196 °C with or without initial cooling to -80 °C. The tardigrades easily survive these temperatures, yet with a significant decrease in viability following rapid cooling by direct exposure to -196 °C. Hence, post-freeze recovery of R. varieornatus seems to rely on cooling rate and thus controlled ice formation. Cryophilic organisms are renowned for having cold-active enzymes that secure appropriate reaction rates at low temperatures. Hence, extreme freeze-tolerance in R. varieornatus could potentially involve syntheses of cryoprotectants and de novo transcription. We therefore generated a reference transcriptome for this cryophilic R. varieornatus population and explored for differential gene expression patterns following cooling to -80 °C as compared to active 5 °C controls. Specifically, we tested for fast transcription potentially occurring within 25 min of cooling from room temperature to a supercooling point of ca. -20 °C, at which the tardigrades presumably freeze and enter into the ametabolic state of cryobiosis. Our analyses revealed no evidence for differential gene expression. We, therefore, conclude that extreme freeze-tolerance in R. varieornatus relies on controlled extracellular freezing with any freeze-tolerance related genes being constitutively expressed.


Subject(s)
Ice , Tardigrada , Animals , Cold Temperature , Freezing , Tardigrada/genetics , Temperature
19.
Article in English | MEDLINE | ID: mdl-35182765

ABSTRACT

Tardigrades are renowned for their extreme stress tolerance, which includes the ability to endure complete desiccation, high levels of radiation and very low sub-zero temperatures. Nevertheless, tardigrades appear to be vulnerable to high temperatures and thus the potential effects of global warming. Here, we provide the first analysis of transcriptome data obtained from heat stressed specimens of the eutardigrade Ramazzottius varieornatus, with the aim of providing new insights into the molecular processes affected by high temperatures. Specifically, we compare RNA-seq datasets obtained from active, heat-exposed (35 °C) tardigrades to that of active controls kept at 5 °C. Our data reveal a surprising shift in transcription, involving 9634 differentially expressed transcripts, corresponding to >35% of the transcriptome. The latter data are in striking contrast to the hitherto observed constitutive expression underlying tardigrade extreme stress tolerance and entrance into the latent state of life, known as cryptobiosis. Thus, when examining the molecular response, heat-stress appears to be more stressful for R. varieornatus than extreme conditions, such as desiccation or freezing. A gene ontology analysis reveals that the heat stress response involves a change in transcription and presumably translation, including an adjustment of metabolism, and, putatively, preparation for encystment and subsequent diapause. Among the differentially expressed transcripts we find heat-shock proteins as well as the eutardigrade specific proteins (CAHS, SAHS, MAHS, RvLEAM, and Dsup). The latter proteins thus seem to contribute to a general stress response, and may not be directly related to cryptobiosis.


Subject(s)
Tardigrada , Transcriptome , Animals , Heat-Shock Proteins/genetics , Heat-Shock Response/genetics , RNA-Seq , Tardigrada/genetics
20.
Cien Saude Colet ; 27(1): 173-182, 2022 Jan.
Article in Portuguese, English | MEDLINE | ID: mdl-35043897

ABSTRACT

This study aimed to identify and analyze the themes of body culture mobilized in the interventions of Physical Education professionals working in Psychosocial Care Centers (CAPS) and understand their presence in urban spaces outside the CAPS. We collected data through semi-structured interviews with 18 Physical Education professionals, members of the eight CAPS teams in Goiânia (GO), Brazil. Data were analyzed using the content analysis technique. The results indicated that the interventions involve different themes. In the social imagination, the activities are instead related to the perspective of physical fitness development, and "sports" and "physical and gymnastic exercises" were the most reported. However, there is a scope for others that are less marked by the expected physical fitness development.


O objetivo desta pesquisa foi identificar e analisar os temas da cultura corporal mobilizados nas intervenções dos profissionais de Educação Física que trabalham em Centros de Atenção Psicossocial (CAPS) e compreender a presença destes centros nos espaços urbanos exteriores aos CAPS. Realizamos a coleta de dados através de entrevistas semiestruturadas com 18 profissionais de Educação Física, integrantes das equipes de oito CAPS da cidade de Goiânia, Goiás. Os dados foram analisados a partir da técnica de análise de conteúdo. Os resultados indicaram que as intervenções envolvem temáticas diversas. As atividades que no imaginário social estão mais relacionadas com a perspectiva de desenvolvimento da aptidão física estão presentes, com "esportes" e "exercícios físicos e ginásticos" os mais relatados. Entretanto, percebe-se uma abrangência para outras que são menos marcadas pela expectativa de desenvolvimento da aptidão física.


Subject(s)
Psychiatric Rehabilitation , Brazil , Humans , Physical Education and Training
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