Cell adhesion in microchannel multiple constrictions - Evidence of mass transport limitations.

Biofilm growth (fouling) in microdevices is a critical concern in several industrial, engineering and health applications, particularly in novel high-performance microdevices often designed with complex geometries, narrow regions and multiple headers. Unfortunately, on these devices, the regions with local high wall shear stresses (WSS) also show high local fouling rates. Several explanations have been put forward by the scientific community, including the effect of cell transport by Brownian motion on the adhesion rate. In this work, for the first time, both WSS and convection and Brownian diffusion effects on cell adhesion were evaluated along a microchannel with intercalate constriction and expansion zones designed to mimic the hydrodynamics of the human body and biomedical devices. Convection and Brownian diffusion effects were numerically studied using a steady-state convective-diffusion model (convection, diffusion and sedimentation). According to the numerical results, the convection and Brownian diffusion effects on cell adhesion are effectively more significant in regions with high WSS. Furthermore, a good agreement was observed between experimental and predicted local Sherwood numbers, particularly at the entrance and within the multiple constrictions. However, further mechanisms should be considered to accurately predict cell adhesion in the expansion zones. The described numerical approach can be used as a way to identify possible clogging zones in microchannels, and defining solutions, even before the construction of the prototype.

In utero sensitization in Chagas' disease leads to altered lymphocyte phenotypic patterns in the newborn cord blood mononuclear cells.

Neonates are sensitized in utero to maternal circulating antigens and idiotypes that eventually cross the placental barrier. We believe that children born of mothers under long lasting antigenic stimulation, as in a chronic infection, would be affected by these maternal influences and show differences in the phenotypic repertoire of lymphocytes. To test this hypothesis, we evaluated flow cytometry studies in cord blood mononuclear cells (CBMC) from children born of chagasic mothers without congenital disease, with special attention to T and B cells and expression of activation markers. We have also evaluated the peripheral blood mononuclear cells (PBMC) of these children 6 months after delivery. We show that CBMC of children born of infected mothers have high proliferative responses to antigenic stimulation, significantly lower mean percentages of CD3+ T cells, CD4+ T cells and diminished expression of the costimulatory molecule CD28 in the CD8+ T cell subset. Interestingly, this subpopulation has an increased expression of the MHC class II gene product as evidenced by the expression of HLADR. It is noteworthy that the patterns observed in CBMC T lymphocyte populations of these children closely resemble earlier findings on lymphocytic profiles of chronic chagasic adult patients and those of their mothers. We also show that, 6 months after delivery, some alterations observed at birth are reversed to levels observed in noninfected individuals.