Persistence of pathogens and bacterial community dynamics in tropical soil after application of raw sewage.

The objective of this work was to evaluate the persistence of faecal indicators and pathogenic organisms (Salmonella spp., Escherichia coli and viable helminth eggs) and the structure/diversity of bacterial communities in soil receiving raw sewage (RS) for an extended period of application (3 uninterrupted years). In the experimental design, three treatments were defined: (1) Control soil, characterized by the analysis of a composite sample collected in an area of similar soil, but not a recipient of RS (TSC); (2) Soil receiving conventional mineral fertilization, and furrow irrigation with supply water (TW); and (3) Fertirrigated soil with RS applied by furrows (TF). The results of persistence of pathogenic organisms and indicators in TF indicated a sanitary quality similar to the control soil (TSC), thus potentially bringing low risks of contamination with pathogens present in the soil. The presence of viable helminth eggs was not identified in any treatment studied, because of its low concentration in the raw sewage of the studied system. The TW, TF and TSC treatments had 34.8% of bacterial diversity in common. The bacterial composition of the soil showed a predominance of the Proteobacteria phylum in all treatments studied; however, TF was the one with the highest relative abundance of this phylum (44.8%).

Macronutrient distribution in soil subjected to raw sanitary sewage application by closed-end furrows.

This work aimed to evsaluate the influence of raw sanitary sewage (RS) application in closed-end and level-bottom furrows on the distribution of macronutrients and sodium in the soil along its depth. The applied amount of RS was controlled due to the harmful effect of sodium, with a weekly application over three consecutive years. The experimental design consisted of two experimental plots receiving RS, one with alternating the site of effluent application (alternation of inlet and outlet-TFA) and the other without alternating the site of application (only inlet-TFN); and two experimental plots receiving conventional mineral fertilization, both irrigated with public water supply, where one alternated the site of water application (alternation of inlet and outlet-TWA) and the other without alternating the site of application (only inlet-TWN). The inversion of the flow direction with RS application in the closed-end furrows (TFA) provided a better distribution of nutrients in the soil along its length. There was no significant difference in the levels of macronutrients and sodium between the initial and final regions of the furrow soil. In contrast, the fixed flow direction experimental unit (TFN) exhibit a significant difference between the initial and final levels of total nitrogen, phosphorus, potassium and sodium in the closed furrows with mean concentrations of 265.2, 16.2, 46.7 and 110.0 mg dm-3, respectively.

Effect of the association of coagulation/flocculation, hydrodynamic cavitation, ozonation and activated carbon in landfill leachate treatment system.

Mature landfill wastewater is a complex effluent due to its low biodegradability and high organic matter content. Currently, mature leachate is treated on-site or transported to wastewater treatment plants (WWTPs). Many WWTPs do not have the capacity to receive mature leachate due to its high organic load leading to an increase in the cost of transportation to treatment plants more adapted to this type of wastewater and the possibility of environmental impacts. Many techniques are used in the treatment of mature leachates, such as coagulation/flocculation, biological reactors, membranes, and advanced oxidative processes. However, the isolated application of these techniques does not achieve efficiency to meet environmental standards. In this regard, this work developed a compact system that combines coagulation and flocculation (1st Stage), hydrodynamic cavitation and ozonation (2nd Stage), and activated carbon polishing (3rd Stage) for the treatment of mature landfill leachate. The synergetic combination of physicochemical and advanced oxidative processes showed a chemical oxygen demand (COD) removal efficiency of over 90% in less than three hours of treatment using the bioflocculant PGα21Ca. Also, the almost absolute removal of apparent color and turbidity was achieved. The remaining CODs of the treated mature leachate were lower when compared to typical domestic sewage of large capitals (COD ~ 600 mg L-1), which allows the interconnection of the sanitary landfill to the urban sewage collection network after treatment in this proposed system. The results obtained with the compact system can help in the design of landfill leachate treatment plants, as well as in the treatment of urban and industrial effluents which contains different compounds of emerging concern and persistence in the environment.

Characterisation and perspectives of energetic use of dissolved gas recovered from anaerobic effluent with membrane contactor.

Biogas is a source of renewable energy, and its production and use has been validated in anaerobic-based sewage treatment plants (STPs). However, in these systems, a large amount of methane is lost as dissolved methane (D-CH4) in the liquid effluent. In this study, the characteristics and potential energetic uses of the gas recovered during the desorption of D-CH4 from anaerobic effluents with hollow fibre membrane contactors were investigated. A pilot-scale experiment was performed using sewage and two types of membrane contactors. The recovered gas contained considerable amounts of CH4, CO2, H2S, N2, and O2; therefore, a gas upgrade is required prior to its use as a biofuel. The recovery process should be energetically self-sustainable, and induce a considerable decrease in the STP carbon footprint. Recovering D-CH4 with membrane contactors could increase the energetic potential of anaerobic-based STPs up to 50 % and allow for more sustainable systems.

Kinetics and mineralization fraction of organic matter from sewage sludge mixed with soil under controlled laboratory conditions.

The objective of this work was to evaluate the kinetics and mineralization fraction of organic matter from sewage sludge mixed with the soil under controlled laboratory conditions. For this, organic material samples accumulated in layers 0-5 cm, 5-10 cm and 10-15 cm in relation to the surface of a vertical flow constructed wetland system (VF-CW) used for treatment of septic tank sludge, in addition to samples of dewatered sludge from a septic tank and a UASB reactor and non-dewatered sludge from an anaerobic digester were mixed with material collected from the surface layer of a Red Yellow Argisol at rates equivalent to the applications, via organic residues, of 300 and 600 kg ha-1 year-1 of total nitrogen. It was found that the two-phase kinetic equation fit better to the mineralization data of labile and recalcitrant organic carbon. For the lowest nitrogen application rate in the mixtures, the mineralization fractions of the total organic carbon were higher than 73%, while at the highest dose there was a reduction in the mineralization of organic matter by 17% and 63%, respectively in samples collected in layer 10-15 cm from the VF-CW and in the septic tank sludge dewatered in the drying bed.

Randomised Controlled Trial of Fish Oil Supplementation on Responsiveness to Resistance Exercise Training in Sarcopenic Older Women.

This study aims to investigate the effects of fish oil supplementation on the muscle adaptive response to resistance exercise training, physical performance and serum levels of inflammatory cytokines in sarcopenic older women. A randomised, double-blind, placebo-controlled trial is performed with thirty-four sarcopenic women (2010 European Consensus of Sarcopenia), aged ≥ 65 years. The participants are allocated into the following two groups: Exercise and Fish Oil (EFO) and Exercise and Placebo (EP). Both groups undertook a resistance exercise programme over 14 weeks. All participants are instructed to ingest 4 g/day of food supplements; the EP group received sunflower oil capsules, and the EFO group, fish oil capsules. The cross-sectional area (CSA) of the quadriceps muscle is calculated using magnetic resonance imaging (MRI). The strength of the lower limbs is measured using isokinetic dynamometry. Both groups show improvements in CSA and strength after the intervention. Changes in EFO are significantly greater compared with EP for muscle strength (peak torque, 19.46 Nm and 5.74 Nm, respectively, p < 0.001). CSA increased after the intervention in both groups (EFO; 6.11% and EP; 2.91%), although there is no significant difference between the groups (p = 0.23). There are no significant intra-group, inter-group or time differences in any of the cytokines measured. The use of fish oil supplementation potentiates the neuromuscular response to the anabolic stimulus from training, increasing muscle strength and physical performance in sarcopenic older women.

Prevalence and factors associated with depression in the elderly: a cross-sectional study / Prevalência e fatores associados à depressão em idosos: um estudo transversal

ABSTRACT: Introduction: Depression is a psychiatric disease that causes losses in the biopsychosocial aspect of the affected individuals. The prevalence in the elderly population is significant, and identify the factors related to this outcome allows preventive measures and treatment can be early applied. Objective: Assessing the association between depression and sociodemographic and health risk factors in the elderly. Methods: This is a cross-sectional study involving 388 elderly of both genders, aged 65 years and older, urban residents of the city of Cuiabá, state of Mato Grosso (MT), Brazil. A socio-demographic questionnaire, as well as a self-report of chronic diseases and lifestyle, a self-report questionnaire of functional capacity, occurrence of falls, Geriatric Depression Scale, mental state assessment, and anthropometry tests were used. The chi-square test, oddsratio (OR), and multiple logistic regression with 95% confidence interval and p<5% were used in the statistical analysis. Results: There was a predominance of women (64%) who were mulatto/caboclo/brown skin (43%) and married (45%). Depression was significantly associated with: the lowest level of education, <8 years (OR=2.15; p=0.012), partial or total dependence of instrumental activities of daily living (OR=1.85; p=0.003), having five or more diseases (OR=2.93, p=0.002), falls in the last twelve months (OR=1.90; p=0.021), and sleep (OR=2.39; p<0.001) and visual difficulties (OR=2.28; p<0.001). Conclusion: Depression in this group of elderly was associated with modifiable and preventable factors in the health of these people. Thus, the early diagnosis of risk factors associated with depression, the inclusion of social, physical and cognitive activities must be considered for their prevention. (AU)

RESUMO: Introdução: Depressão é uma doença psiquiátrica e provoca prejuízos no aspecto biopsicossocial dos indivíduos acometidos. Sua prevalência na população idosa é expressiva, a identificação dos fatores relacionados a este desfecho permitem que medidas preventivas e de tratamento sejam aplicadas precocemente. Objetivo: Avaliar a associação entre depressão, os fatores sociodemográficos e os riscos para a saúde em idosos. Métodos: Estudo transversal com 388 idosos de ambos os sexos com idade igual ou superior a 65 anos, residentes do município de Cuiabá, estado de Mato Grosso (MT), Brasil. Foram usados questionários sociodemográficos, instrumentos de autorrelato de doenças crônicas, de estilo de vida, ocorrência de quedas e de capacidade funcional, escala de depressão geriátrica, avaliação do estado mental e testes de antropometria. Na análise estatística utilizou-se teste do qui-quadrado, odds ratio (OR) e regressão logística múltipla com intervalo de confiança de 95% e p<5%. Resultados: Encontrou-se predominância de mulheres (64%), sendo mulata/cabocla/parda (43%) e casadas (45%). A depressão foi significativamente associada com: o tercil mais baixo de educação <8 anos (OR=2,15; p=0,012); com dependência parcial ou total de atividades instrumentais da vida diária (OR=1,85; p=0,003); ter cinco ou mais doenças crônicas (OR=2,93; p=0,002), ocorrência de quedas nos últimos doze meses (OR=1,90; p=0,021); dificuldades de sono (OR=2,39; p<0,001) e visual (OR=2,28; p<0,001). Conclusão: A depressão estava associada a fatores modificáveis e que podem ser prevenidos. Desta forma, o diagnóstico precoce destes fatores de risco, a inclusão de atividades socias, físicas e cognitivas devem ser consideradas para sua prevenção. (AU)

Stephalagine, an aporphine alkaloid from Annona crassiflora fruit peel, induces antinociceptive effects by TRPA1 and TRPV1 channels modulation in mice.

Pain relief represents a critical unresolved medical need. Consequently, the search for new analgesic agents is intensively studied. Annona crassiflora, a native species of the Brazilian Savanna, represents a potential source for painful treatment. This study aimed to investigate the antinociceptive potential of A. crassiflora fruit peel, focusing on its major alkaloid, stephalagine, in animal models of pain evoked by the activation of transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) channels. Male C57BL/6/J mice were submitted to formalin-, cinnamaldehyde-, and capsaicin-induced nociception tests to assess nociceptive behavior, and to the open-field and rotarod tests for motor performance analyses. Moreover, the stephalagine's effect was tested on capsaicin- and cinnamaldehyde-induced Ca2+ influx in spinal cord synaptosomes. In silico assessments of the absorption, distribution, metabolism and central nervous system permeability of stephalagine were carried out. The ethanol extract and alkaloidal fraction reduced the nociception induced by formalin. When administered by oral route (1 mg/kg), stephalagine reduced the spontaneous nociception and paw edema induced by TRPV1 agonist, capsaicin, and by TRPA1 agonists, cinnamaldehyde- and formalin, without altering the animals' locomotor activity. The prediction of in silico pharmacokinetic properties of stephalagine suggests its capacity to cross the blood-brain barrier. Furthermore, this alkaloid reduces the capsaicin- and cinnamaldehyde-mediated Ca2+ influx, indicating a possible modulation of TRPV1 and TRPA1 channels, respectively. Together, our results support the antinociceptive and anti-edematogenic effects of the A. crassiflora fruit peel and suggest that these effects are triggered, at least in part, by TRPV1 and TRPA1 modulation by stephalagine.

Constant and random practice on learning of volleyball serve / Prática constante e aleatória na aprendizagem do saque do voleibol

Abstract The type of practice can influence what is learned from a motor skill. The aim of this study was to investigate the effects of the constant and random practice on the learning of Generalized Motor Program and parameters of the volleyball serve. The sample was composed of 20 children between 10 and 12 years old. The participants performed a pre-test whose score was adopted to counterbalance two groups (n=10), random practice and constant practice. During the acquisition phase, the random group performed 252 serves from three different positions, always indicated at the end of each serve, and the constant group performed all serves from only a specific position. The retention test showed that both groups learned the Generalized Motor Program, but random practice conducted to higher parameterization learning, resultant from the variable of practice. During practice of a sport motor skills, although the constant and random practice improve the learning of Generalized Motor Program, only the random practice improves learning of parameters of the motor skill.

Resumo O tipo de prática pode influenciar o que é aprendido de uma habilidade motora. Objetivou-se investigar os efeitos da prática constante e aleatória na aprendizagem do Programa Motor Generalizado e parâmetros do saque do voleibol. A amostra foi composta por 20 crianças entre 10 e 12 anos. Os participantes realizaram um pré-teste, cujo escore foi utilizado para contrabalançar dois grupos (n=10), prática constante e aleatória. Durante a fase de aquisição, o grupo aleatório realizou 252 saques de três posições diferentes, sempre indicada ao final de cada saque e o grupo controle realizou todos os saques somente de uma posição específica. O teste de retenção mostrou que ambos os grupos aprenderam o Programa Motor Generalizado, mas a prática aleatória conduziu para uma melhor aprendizagem dos parâmetros, resultante da prática variada. Durante a prática de habilidades motoras esportivas, apesar de as práticas constante e aleatória melhorarem a aprendizagem do Programa Motor Generalizado, somente a prática aleatória melhora a aprendizagem dos parâmetros da habilidade motora.

Prevalence and factors associated with sarcopenia and dynapenia in elderly people.

OBJECTIVES: There is little information about the risk factors for sarcopenia and dynapenia. This study aimed to assess the prevalence of sarcopenia and dynapenia and to verify which risk factors are associated with the elderly population. METHODS: A total of 387 elderly people were evaluated. We used a questionnaire to identify socio-demographic and behavioral aspects. For physical performance, we used the Short Physical Performance Battery. Using the European Working Group of Sarcopenia in Older People consensus, we defined sarcopenia that includes the occurrence of low muscle mass, added to low muscle strength or low physical performance. Dynapenia was defined using handgrip strength. RESULTS: Sarcopenia and dynapenia were identified in 15.3% and 38.2% of the elderly people, respectively; 15.8% of women and 14.2% of men had sarcopenia, and 52.4% of women and 13.5% of men had dynapenia. Sarcopenia was associated with the increase in aging, white race, smoking, and risk of malnutrition. Dynapenia is more likely to occur in women and hospitalized patients. CONCLUSION: Sarcopenia had a greater association with the risk factors evaluated here, mainly with smoking and nutritional status. On the other hand, dynapenia was different, having a greater association with hospital intervention.

Correlation between muscle mass, nutritional status and physical performance of elderly people.

OBJECTIVES: This study evaluated the relationship between the skeletal muscle mass (SMM), obtained by predictive equations, and the body composition, nutritional aspects, functionality and physical performance in elderly people. METHODS: The sample consisted of adults aged 65 years or over from the cross-sectional study of the Brazilian Elderly Frailty Study Network, in Cuiabá, Mato Grosso State, Brazil. The anthropometric parameters, instrumental activities of daily living (IADL), Short Physical Performance Battery (SPPB), and handgrip strength (HGS) were evaluated. The SMM was estimated by 2 predictive anthropometric equations. RESULTS: Both SMM equations correlated with age, anthropometric indices, SPPB, IADL, and HGS. However, only HGS and neck circumference strongly correlated in both equations, being higher in SMM II. CONCLUSIONS: It seems that both equations are sensitive to obtain the SMM, contributing to the diagnosis of sarcopenia, nutritional status, and a physical performance condition.

Membrane lipid profile of in vitro-produced embryos is affected by vitrification but not by long-term dietary supplementation of polyunsaturated fatty acids for oocyte donor beef heifers.

Dietary rumen-protected polyunsaturated fatty acids (PUFAs) rich in linoleic acid (LA) may affect embryo yield, and LA can modulate the molecular mechanisms of lipid uptake in bovine blastocysts produced in vitro. In embryos, membrane lipids, such as phosphatidylcholines (PCs) and sphingomyelins (SMs), affect cryopreservation success. The aim of the present study was to evaluate embryonic developmental rates after the IVF of oocytes retrieved from Nellore heifers fed for approximately 90 days with rumen-protected PUFAs rich in LA. In addition, we evaluated embryo cryotolerance and the membrane structure lipid composition using matrix-assisted laser desorption ionisation mass spectrometry of fresh and vitrified embryos. Embryo development to the blastocyst stage (mean 43.2%) and embryo survival after vitrification and warming (mean 79.3%) were unaffected by diet. The relative abundance of one lipid species (PC ether (PCe; 38:2, which means that this lipid has 38 carbon atoms and 2 double bonds in the fatty acyl residues) was increased after PUFAs supplementation. However, 10 ions were affected by cryopreservation; ions consistent with PC 32:0, PC 34:1, SM 24:1, PC 40:6 or PC 42:9, PC plasmalogen (PCp) 44:10 or PC 42:7, triacylglycerol (TAG) 54:9 and a not assigned ion (m/z 833.2) were lower in blastocysts that survived to the cryopreservation process compared with fresh blastocysts, whereas the abundance of the ions PC 36:3 or PC 34:0, PCe 38:2 or PC 36:6 and PC 36:5 or PCe 38:1 were increased after cryopreservation. Thus, the results demonstrate that the mass spectrometry profiles of PC, SM and TAG species differ significantly in bovine blastocysts upon cryopreservation. Because the lipid ion abundances of fresh and vitrified-warmed embryos were distinct, they can be used as potential markers of post-cryopreservation embryonic survival.

iHEART: a miniaturized near-infrared in-line gas sensor using heart-shaped substrate-integrated hollow waveguides.

A novel heart-shaped substrate-integrated hollow waveguide (hiHWG) was integrated with a near-infrared micro-spectrometer (µNIR) for sensing natural gases, resulting in an ultra-compact near-infrared gas sensing system - iHEART. The iHEART system was evaluated using two different µNIR spectrometers, and the performance was compared with a laboratory NIR spectrometer for gas analysis based on an acousto-optic tunable filter (AOTF). The spectral data were pre-processed using the 1(st) derivative Savitzky-Golay algorithm, and then used for establishing multivariate regression models based on partial least squares (PLS). The root mean square errors of prediction (RMSEPs) obtained for major components of natural gas with both iHEART systems were similar to those associated with the AOTF spectrophotometer combined with a conventional long-path measurement cell. It was demonstrated that the iHEART system has significant potential for the development of compact in-line gas sensing systems, thus facilitating monitoring of (petro)chemically relevant processes and products. However, the flexibility and modularity of the system also allows tailoring iHEART to a wide range of other relevant analytical measurement scenarios requiring short response times and minute gas sample volumes.

Concordância entre diferentes equações antropométricas na predição de gordura corporal em idosos / Concordance between different anthropometric equations for predicting body fat in the elderly / Concordancia entre las diferentes ecuaciones antropométricas para la predicción de la grasa corporal en personas de edad avanzada

O objetivo foi verificar a concordância entre as diferentes equações antropométricas desenvolvidas para estimativa do percentual de gordura corporal (%G) em idosos de ambos os sexos. Avaliaram-se 141 homens e 244 mulheres, ≥65 anos. Foram realizadas medidas de estatura, massa corporal e perímetros corporais. Nove equações baseadas em medidas antropométricas foram testadas. Utilizou-se o coeficiente de correlação intra-classe, análise da concordância das equações e a análise gráfica de Bland e Altman. Nos homens idosos, a equação de Tran, & Weltman, utilizada como referencial, subestimou e diferiu o %G, comparada com todas as outras equações. A equação de Deurenberg, et al., contudo, demonstrou concordância de 78% e forte relação (r=0,78). Nas mulheres, superestimou-se o %G, em que apenas a equação de Visser, et al. não diferiu, tendo concordância de 92% e relação forte (r=0,85). Contudo, essas equações antropométricas que apresentaram melhores resultados, podem contribuir no acompanhamento adequado da composição corporal de idosos, sendo mais fácil e viável obterem-se as medidas para estudos populacionais. Sugere-se cautela, entretanto, em sua utilização para a estimativa do %G e futuros estudos com métodos considerados padrões-ouro para validação dessas equações nas diferentes regiões do país, devido a um maior grau de diferenciação étnica e cultural dos idosos existentes no Brasil. Inúmeros fatores de uma população, incluindo idade, raça, etnia, gênero e obesidade devem ser considerados ao selecionar uma equação de regressão para estimar %G. Além disso, existe uma necessidade de se desenvolverem modelos de regressão precisos que considerem obesidade, sexo, raça ou etnia, quando prevendo %G em uma população idosa sul-americana.

The purpose was to investigate the concordance between the different anthropometric equations for estimation of body fat percentage (BF%) in elderly men and women. Evaluated 141 men and 244 women, ≥65 years. It was held height measurements, body mass and body perimeters. Nine equations based on anthropometric measures were tested. We use coefficient of intra-class correlation, concordance analysis of the equations and graphical analysis of Bland-Altman. In older men the equation Tran & Weltman utilized as reference, underestimated and differed the %BF compared to all other equations, however, the equation of Deurenberg, et al. demonstrated 78% concordance and strong relationship (r=0.78). In older women overestimated %BF, the equation of Visser, et al. not differed and had 92% concordance and strong correlation (r=0.85). However, these anthropometric equations showed better results can ontribute in appropriate monitoring of body composition in elderly, being easier and viable to obtain measures for population studies. It is suggested, however, caution in its use for the estimation of% G and future studies with methods considered gold standards for validation of these equations in the different regions of the country, due to a greater degree of ethnic and cultural differentiation of the elderly in Brazil. Numerous factors of a population, including age, race, ethnicity, gender and obesity should be considered when selecting a regression equation to estimate% G. In addition, there is a need to develop accurate regression models that consider obesity, gender, race or ethnicity when predicting% G in an elderly South American population.

El objetivo fue investigar la concordancia entre las diferentes ecuaciones antropométricas para la estimación del porcentaje de grasa corporal (% BF) en hombres y mujeres ancianos. Se evaluaron 141 hombres y 244 mujeres, ≥65 años. Se realizaron mediciones de altura, masa corporal y perímetros corporales. Se probaron nueve ecuaciones basadas en medidas antropométricas. Utilizamos el coeficiente de correlación intra-clase, el análisis de concordancia de las ecuaciones y el análisis gráfico de Bland-Altman. En los hombres mayores, la ecuación Tran, & Weltman utilizó como referencia, subestimó y diferenció el% BF en comparación con todas las demás ecuaciones, sin embargo, la ecuación de Deurenberg, et al. Demostró 78% de concordancia y fuerte relación (r = 0,78). En las mujeres mayores sobrestimado% BF, la ecuación de Visser, et al. No difirió y tuvo concordancia del 92% y fuerte correlación (r = 0,85). Sin embargo, estas ecuaciones antropométricas mostraron mejores resultados que pueden contribuir a un monitoreo adecuado de la composición corporal en ancianos, siendo más fácil y viable obtener medidas para estudios poblacionales. Se sugiere, sin embargo, precaución en su uso para la estimación de% G y futuros estudios con métodos considerados estándares de oro para la validación de estas ecuaciones en las diferentes regiones del país, debido a un mayor grado de diferenciación étnica y cultural de los ancianos en Brasil . Se deben considerar numerosos factores de una población, incluyendo la edad, la raza, la etnia, el género y la obesidad al seleccionar una ecuación de regresión para estimar% G. Además, es necesario desarrollar modelos de regresión precisos que consideren la obesidad, el género, la raza o la etnia Al predecir% G en una población sudamericana de edad avanzada.

Antinociceptive and anti-inflammatory potential of extract and isolated compounds from the leaves of Salvia officinalis in mice.

ETHNOPHARMACOLOGICAL RELEVANCE: Salvia officinalis L. has been used as a traditional herbal medicine for gastric disturbances and inflammatory processes. This study investigated the toxicological, antinociceptive and anti-inflammatory effects of the hydroalcoholic extract (HE) from leaves of Salvia officinalis and its isolated compounds in mice. MATERIALS AND METHODS: Mice were treated with HE before the induction of nociceptive response by chemical agents (acetic-acid, formalin, glutamate, capsaicin and cinnamaldehyde). Total leukocytes and plasma extravasation induced by acetic acid and paw oedema induced by glutamate, capsaicin and cinnamaldehyde were also measured. The antinociceptive effect of carnosol and ursolic acid/oleanolic acid were evaluated on formalin and cinnamaldehyde models. RESULTS: In the acute toxicity test the value of estimated LD50 for HE was 44.7579 g/kg. Oral administration of HE (10, 30 and 100 mg/kg) inhibited the number of writhings, total leukocytes and plasma extravasation induced by acetic acid. In the formalin test, HE reduced both neurogenic and inflammatory phases, effect that was affected by naloxone. The glutamate-, capsaicin- and cinnamaldehyde-induced nociception and paw oedema were reduced by HE at doses that did not affect the locomotor activity of mice in the open field test. Carnosol (10mg/kg) and ursolic acid/oleanolic acid (30 mg/kg) inhibited the inflammatory phase of formalin and the nociception and mechanical allodynia induced by cinnamaldehyde. CONCLUSIONS: These results demonstrate that HE presents significant anti-inflammatory and also antinociceptive effects on chemical behavioral models of nociception that involves an opioid mechanism. In addition, carnosol and ursolic acid/oleanolic acid contained in this plant appears to contribute for the antinociceptive property of the extract, possibly through a modulatory influence on TRPA1-receptors. However, further studies regarding the precise site and the mechanism of action of HE and carnosol and ursolic acid/oleanolic acid merited exploring further.