ABSTRACT
Bacterial contamination of staining reagents led to false-positive smears from clinical samples. Piped deionized water used to make up the staining reagents was found to be the source of contamination. The closed loop system supplying the deionized water was decontaminated with hydrogen peroxide and upgraded by addition of a reverse osmosis unit and bacterial filter. No subsequent contamination has been demonstrated.
Subject(s)
Bacterial Infections/microbiology , Body Fluids/microbiology , Gentian Violet , Gram-Negative Bacteria/isolation & purification , Phenazines , Water , Bacterial Infections/cerebrospinal fluid , Disinfection , Equipment Contamination , False Positive Reactions , HumansABSTRACT
Bordetella bronchiseptica and toxigenic type-D Pasteurella multocida were cultured from pigs in each of five herds diagnosed as having severe atrophic rhinitis (AR). B. bronchiseptica alone, P. multocida alone, or both organisms isolated from four herds were inoculated intranasally into 1-week-old gnotobiotic pigs which were necropsied 4 weeks post-inoculation (PI). Nasal turbinate atrophy in B. bronchiseptica-inoculated pigs was moderate to severe, while P. multocida-inoculated pigs had slight to severe atrophy. Pigs inoculated with both organisms had moderate to complete turbinate atrophy. P. multocida was reisolated at necropsy from all pigs receiving the organism except those having no turbinate damage. B. bronchiseptica and P. multocida from a fifth herd were simultaneously inoculated into six naturally farrowed 6-day-old SPF pigs. Necropsy performed 4 weeks PI revealed severe to complete turbinate atrophy. Nasal turbinates were normal for control pigs in both experiments.
