Effective Striga control and yield intensification on maize farms in western Kenya with N fertilizer and herbicide-resistant variety.

Context: Maize production in western Kenya is limited by the spread of parasitic weed Striga hermonthica and depletion of soil nutrient stocks. Nitrogen (N) fertilizer and imidazolinone resistant (IR) maize are key elements in the agronomic toolbox to control infestations and enhance yields. Research question: The circumstances under which their use, individually or combined, is most effective on farmer fields have not been well documented. Inappropriate management decisions and low returns on investments arise from this knowledge gap, causing hunger and poverty in smallholder communities to persist. Methods: Experiments were carried out on 60 fields in three different agroecosystems of western Kenya using full-factorial treatments with non-herbicide treated maize (DH) and herbicide treated maize (IR), and N fertilizer omission and application. Trials were stratified on a field with low and high soil fertility within individual farms and repeated over two seasons. Results: Cultivating IR maize instead of DH maize decreased the emergence of Striga with 13 shoots m-2 on average while applying N fertilizer on DH maize led to a reduction of 5 shoots m-2 on average. Decreases of Striga by use of IR maize and N fertilizer were between 6 and 23 shoots m-2 larger at the site with high levels of infestation than at the sites with medium or low emergence. Input of N fertilizer increased grain harvests by 0.59 ton ha-1 on average while use of IR maize enhanced the productivity with 0.33 ton ha-1 on average. Use of N fertilizer had similar yield effects in all three sites, whereas use of IR maize at the site with high Striga emergence increased maize production by 0.26-0.39 ton ha-1 more than at the sites with medium or low emergence. Conclusions: The greater Striga responses to IR maize and the greater yield responses to N fertilizer demonstrate their use could be optimized according to field conditions and management goals. Combining IR maize and N fertilizer has larger added yield benefits where their individual effects on grain productivity are smaller. Significance: Findings from this study indicate that farmers in western Kenya require guidance on how to align the use of herbicide resistant maize and inorganic N inputs with the level of Striga infestation and maize yield on their fields for effectively controlling the pernicious weed and enhancing food production.

Nutrients Limiting Soybean (glycine max l) Growth in Acrisols and Ferralsols of Western Kenya.

Low soybean yields in western Kenya have been attributed to low soil fertility despite much work done on nitrogen (N) and phosphorus (P) nutrition leading to suspicion of other nutrient limitations. To investigate this, a nutrient omission trial was set up in the greenhouse at the University of Eldoret-Kenya to diagnose the nutrients limiting soybean production in Acrisols from Masaba central and Butere sub-Counties, and Ferralsols from Kakamega (Shikhulu and Khwisero sub-locations) and Butula sub-Counties and to assess the effect of liming on soil pH and soybean growth. The experiment was laid out in a completely randomized design with ten treatments viz; positive control (complete), negative control (distilled water), complete with lime, complete with N, minus macronutrients P, potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) and with, micro-nutrients boron (B), molybdenum (Mo), manganese (Mn), copper (Cu) and zinc (Zn) omitted. Visual deficiency symptoms observed included interveinal leaf yellowing in Mg omission and N addition and dark green leaves in P omission. Nutrients omission resulted in their significantly low concentration in plant tissues than the complete treatment. Significantly (P≤ 0.05) lower shoot dry weights (SDWs) than the complete treatment were obtained in different treatments; omission of K and Mg in Masaba and Shikhulu, Mg in Khwisero, K in Butere and, P, Mg and K in Butula. Nitrogen significantly improved SDWs in soils from Kakamega and Butula. Liming significantly raised soil pH by 9, 13 and 11% from 4.65, 4.91 and 4.99 in soils from Masaba, Butere and Butula respectively and soybean SDWs in soils from Butere. The results show that, poor soybean growth was due to K, Mg and P limitation and low pH in some soils. The results also signify necessity of application of small quantities of N for initial soybean use.

Shoot epicatechin and epigallocatechin contents respond to water stress in tea [Camellia sinensis (L.) O. Kuntze].

An experiment was conducted to determine the association of tea catechins to water stress in tea, with the objective of determining their suitability as indicators for predicting drought tolerance in tea (Camellia sinensis). The study consisted of six tea clones (BBK 35, TRFK 6/8, TRFK 76/1, TRFK 395/2, TRFK 31/30, and TRFK 311/287) and four levels of soil water content (38, 30, 22, and 14% v/v), which were arranged in a complete randomized design and replicated 3 times. The treatments were maintained for a period of 12 weeks. Tea shoots were sampled for catechin analysis during the 6th week of water treatment, in which fresh shoots with two leaves and a bud were plucked and steamed for 2 min, and dried at 70 degrees C to constant weight. Subsequently, the samples were ground and analyzed for catechins using an HPLC system. The total catechins showed significant correlation with shoot growth (r=0.65, P=0.006), soil water content (r=0.54, P=0.0066), and water stress index (r=0.67, P=0.0004). The epicatechin (EC) correlated with shoot growth (r=0.58, P=0.0032), soil water content (r=0.62, P=0.0014), and water stress index (r=0.63, P=0.0010). Similarly, epigallocatechin (EGC) correlated with shoot growth (r=0.65, P=0.0006), soil water content (r=0.50, P=0.0133), and water stress index (r=0.60, P=0.0021). However, epigallocatechin gallate (EGCg) and epicatechin gallate (ECG) showed no significant response to changes in soil water content. The shoot contents of EC and EGC in the six clones showed varied responses, with a distinct pattern in the water-stress tolerant clones (TRFK 6/8 and TRFK 31/30). The results suggest a potential use for EC and EGC as indicators in predicting drought tolerance in tea.

Polyphenols as potential indicators for drought tolerance in tea (Camellia sinensis L.).

Plant polyphenols have gained prominence in quality of plant products and in human health. An experiment was conducted to determine the association of tea polyphenols with water stress and their suitability as indicators for drought tolerance. The experiment was conducted in a 'rain-out' shelter, and consisted of six tea clones (BBK 35, TRFK 6/8, TRFK 76/1, TRFK 395/2, TRFK 31/30, and TRFK 311/287) and four levels of soil water contents (38, 30, 22, and 14% v/v), which were maintained for a period of 12 weeks. The treatments were arranged in a completely randomized design and replicated three times. Plant growth was monitored over 6 weeks, and a water stress index was calculated to determine water-stress tolerant clones. Total polyphenols in tea shoots was analyzed and a regression analysis done. The results indicate that declining soil water content (SWC) reduced both growth and content of polyphenols in tea. Tolerant clones maintained a high polyphenol content at low SWC, and also showed less fluctuation in phenolics when subjected to changes in SWC. There was significant (P<0.001) correlation of total polyphenol content with shoot growth and WSI of tea, and a linear relationship (r2=0.97) between SWC for tea and both, water stress index and shoot polyphenol content. We report that there is a potential to use polyphenols as indicators for selection of drought-tolerant tea cultivars.