ABSTRACT
Conflicting reports on the toxicity of CeO2 nanomaterials have been published in recent years, with some studies finding CeO2 nanoparticles to be toxic, while others found it to have protective effects against oxidative stress. To investigate the possible reasons for this, we have performed a comprehensive study on the physical and chemical properties of nanosized CeO2 from three different suppliers as well as CeO2 synthesized by us, and tested their toxicity. For toxicity tests, we have studied the effects of CeO2 nanoparticles on a Gram-negative bacterium Escherichia coli in the dark, under ambient and UV illuminations. We have also performed toxicity tests on the marine diatom Skeletonema costatum under ambient and UV illuminations. We found that the CeO2 nanoparticle samples exhibited significantly different toxicity, which could likely be attributed to the differences in interactions with cells, and possibly to differences in nanoparticle compositions. Our results also suggest that toxicity tests on bacteria may not be suitable for predicting the ecotoxicity of nanomaterials. The relationship between the toxicity and physicochemical properties of the nanoparticles is explicitly discussed in the light of the current results.
Subject(s)
Cerium/chemistry , Metal Nanoparticles/chemistry , Diatoms/drug effects , Diatoms/radiation effects , Electron Spin Resonance Spectroscopy , Escherichia coli/drug effects , Escherichia coli/radiation effects , Metal Nanoparticles/toxicity , Metal Nanoparticles/ultrastructure , Microscopy, Electron, Scanning , Ultraviolet RaysABSTRACT
The toxicity of metal oxide nanomaterials and their antimicrobial activity is attracting increasing attention. Among these materials, MgO is particularly interesting as a low cost, environmentally-friendly material. The toxicity of MgO, similar to other metal oxide nanomaterials, is commonly attributed to the production of reactive oxygen species (ROS). We investigated the toxicity of three different MgO nanoparticle samples, and clearly demonstrated robust toxicity towards Escherichia coli bacterial cells in the absence of ROS production for two MgO nanoparticle samples. Proteomics data also clearly demonstrate the absence of oxidative stress and indicate that the primary mechanism of cell death is related to the cell membrane damage, which does not appear to be due to lipid peroxidation.
Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Magnesium Oxide/toxicity , Nanoparticles/toxicity , Reactive Oxygen Species/metabolism , Electron Spin Resonance Spectroscopy , Escherichia coli/genetics , Escherichia coli/radiation effects , Escherichia coli/ultrastructure , Gene Ontology , Lipopolysaccharides/pharmacology , Membrane Proteins/metabolism , Metabolic Networks and Pathways/drug effects , Metabolic Networks and Pathways/radiation effects , Microbial Sensitivity Tests , Nanoparticles/ultrastructure , Particle Size , Spectroscopy, Fourier Transform Infrared , Time Factors , Ultraviolet RaysABSTRACT
We have investigated the effect of ZnO nanoparticle properties on the dye-sensitized solar cell performance. Nanoparticles with different sizes and optical properties were considered. We found that there is a complex relationship between native defects, dye adsorption, charge transport and solar cell performance. The presence of a high concentration of nonradiative defects was found to be detrimental to photovoltaic performance, whereas for radiative defects, samples displaying orange-red defect emission exhibited better performance compared to samples with green defect emission (when the samples had similar emission intensities). Detailed discussion of the nanoparticle properties and their relationship with dye adsorption, electron injection, electron lifetime, electron transport time, and solar cell performance is given.
