Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 19 de 19
Filter
1.
Int J Equity Health ; 16(1): 126, 2017 07 14.
Article in English | MEDLINE | ID: mdl-28705253

ABSTRACT

BACKGROUND: Despite male smokers being dominant in Vietnam, scarce evidence on trends in socioeconomics inequalities among the hardcore male smokers is available in the country. In this study, we aimed at assessing the trends in socioeconomics inequalities among the hardcore smokers in adult male population in Vietnam over a five-year period from 2010 to 2015. METHODS: We used data from two rounds of the Vietnam Global Adult Tobacco Survey (GATS) conducted in 2010 and 2015. We included only men aged 25 years and above in the analysis. We measured socioeconomic inequalities among hardcore smokers by calculating the concentration index. We conducted multiple logistic regression analysis to identify factors associated with hardcore smoking among men aged 25 years and above. RESULTS: The results of this study showed that the prevalence of male hardcore smokers aged 25 years and above in Vietnam was 9.5% in 2010 which increased to 13.1% in 2015. The prevalence of male hardcore smokers declined in the richest group from the 2010 level whereas it increased in the middle, poor and poorest groups. All values of weighted concentration indices indicated that the prevalence of male hardcore smokers occurred more among the poor men in Vietnam in both 2010 and 2015. The socioeconomic inequalities in hardcore smokers increased during 2010 and 2015. Residence in urban areas was significantly associated with higher adult male hardcore smoking in our study. Belonging to the age groups between 40 and 59 years, attaining primary and lower education, being self-employed, belonging to the poorest household group, smoking being allowed at home and no rule for smoking at home were associated with higher risk of being hardcore smoker among adult males in Vietnam. CONCLUSIONS: We found increased trends in socioeconomic inequalities in hardcore smoking among the study population. Our study results indicate that existing smoking secession and tobacco control policy and interventions need to be modified or new policies and interventions should be introduced with the perspective of addressing socioeconomic inequalities to have the desired impact. We recommend implementing specific targeted interventions for vulnerable population groups for better results.


Subject(s)
Health Status Disparities , Smokers , Smoking/epidemiology , Social Class , Tobacco Use Disorder/epidemiology , Adult , Age Factors , Aged , Educational Status , Employment , Family Characteristics , Humans , Logistic Models , Male , Middle Aged , Prevalence , Smoking Cessation , Socioeconomic Factors , Surveys and Questionnaires , Nicotiana , Tobacco Smoking , Urban Population , Vietnam/epidemiology , Vulnerable Populations
2.
Asian Pac J Trop Med ; 9(1): 39-43, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26851784

ABSTRACT

OBJECTIVE: To establish a surveillance in Dong Thap, at the border with Cambodia by assessing the presence of DENV serotypes and CHIKV among patients hospitalized at Dong Thap general hospital. METHODS: Cross-sectional descriptive analysis was conducted on a cohort of 131 patients hospitalized with acute fever and symptoms compatible with dengue or chikungunya. The study was conducted from January 2012 to February 2013. The full clinical picture was established as well as serological and molecular detection. Serological analysis was sequentially performed on blood samples collected on admission and an average of seven days after admission. The detection of IgM antibody to DENV was performed by IgM capture ELISA and the detection of DENV and CHIKV RNA was done by reverse-transcription multiplex PCR. RESULTS: 101 patients out of 131 (77%) were confirmed with dengue. All four dengue serotypes were detected with a predominance of DENV2 and DENV4. No chikungunya infection was detected although reported in neighboring Cambodia. A differential efficiency of serological dengue detection was observed. Efficiency was 29% upon admission and 53% after seven days on the same patients. 30 patients out of 131 (23%) were negative with both DENV and CHIKV. CONCLUSIONS: Dengue is at risk of being underestimated and chikungunya is not systematically detected. Changes in detection and surveillance procedures are therefore discussed to increase efficiency of dengue detection and continue the monitoring the emergence of CHIKV in Dong Thap province and in Vietnam.

3.
Asian Pac J Trop Med ; 8(7): 543-8, 2015 Jul.
Article in English | MEDLINE | ID: mdl-26276285

ABSTRACT

OBJECTIVE: To record the human cases of dengue fever (DF) and investigate the Aedes mosquito species circulating during the Hanoi 2011 DF epidemics. METHODS: 24 different outbreak points were recorded in 8 districts between August and December 2011. RESULTS: 140 patients were hospitalized following dengue diagnostic with a predominance of males (59.3%) and the 15-34 age class. Only DENV-1 (11.27%) and DENV-2 (88.73%) serotypes were detected in human samples. Mosquito sampling performed in and around patients households revealed the predominance of Aedes aegypti (A. aegypti) (95.15%) versus Aedes albopictus (4.85%). CONCLUSIONS: There is a positive correlation between the population density of A. aegypti and the number of human cases and duration of outbreaks. This was not observed for Aedes albopictus. Three pools of A. aegypti were positive with dengue virus, two with DENV-1 and one with DENV-2.

4.
BMJ Open ; 5(4): e006577, 2015 Apr 22.
Article in English | MEDLINE | ID: mdl-25903751

ABSTRACT

OBJECTIVE: The aim of this study was to compare the efficacy of cloth masks to medical masks in hospital healthcare workers (HCWs). The null hypothesis is that there is no difference between medical masks and cloth masks. SETTING: 14 secondary-level/tertiary-level hospitals in Hanoi, Vietnam. PARTICIPANTS: 1607 hospital HCWs aged ≥18 years working full-time in selected high-risk wards. INTERVENTION: Hospital wards were randomised to: medical masks, cloth masks or a control group (usual practice, which included mask wearing). Participants used the mask on every shift for 4 consecutive weeks. MAIN OUTCOME MEASURE: Clinical respiratory illness (CRI), influenza-like illness (ILI) and laboratory-confirmed respiratory virus infection. RESULTS: The rates of all infection outcomes were highest in the cloth mask arm, with the rate of ILI statistically significantly higher in the cloth mask arm (relative risk (RR)=13.00, 95% CI 1.69 to 100.07) compared with the medical mask arm. Cloth masks also had significantly higher rates of ILI compared with the control arm. An analysis by mask use showed ILI (RR=6.64, 95% CI 1.45 to 28.65) and laboratory-confirmed virus (RR=1.72, 95% CI 1.01 to 2.94) were significantly higher in the cloth masks group compared with the medical masks group. Penetration of cloth masks by particles was almost 97% and medical masks 44%. CONCLUSIONS: This study is the first RCT of cloth masks, and the results caution against the use of cloth masks. This is an important finding to inform occupational health and safety. Moisture retention, reuse of cloth masks and poor filtration may result in increased risk of infection. Further research is needed to inform the widespread use of cloth masks globally. However, as a precautionary measure, cloth masks should not be recommended for HCWs, particularly in high-risk situations, and guidelines need to be updated. TRIAL REGISTRATION NUMBER: Australian New Zealand Clinical Trials Registry: ACTRN12610000887077.


Subject(s)
Infection Control/instrumentation , Masks/standards , Occupational Exposure/prevention & control , Occupational Health Services/methods , Personnel, Hospital/statistics & numerical data , Respiratory Protective Devices/standards , Respiratory Tract Infections/prevention & control , Adult , Aged , Australia , Cluster Analysis , Female , Humans , Influenza, Human/prevention & control , Male , Middle Aged , New Zealand , Occupational Exposure/statistics & numerical data , RNA, Viral/isolation & purification , Respiratory Tract Infections/virology
5.
Am J Infect Control ; 43(1): 72-7, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25564127

ABSTRACT

BACKGROUND: This study aimed to examine the knowledge, attitudes, and practices towards the use of facemasks among hospital-based health care workers (HCWs) in Hanoi, Vietnam. METHODS: A qualitative study incorporating 20 focus groups was conducted between August 2010 and May 2011. HCWs from 7 hospitals in Vietnam were invited to participate. RESULTS: Issues associated with the availability of facemasks (medical and cloth masks) and respirators was the strongest theme to emerge from the discussion. Participants reported that it is not unusual for some types of facemasks to be unavailable during nonemergency periods. It was highlighted that the use of facemasks and respirators is not continuous, but rather is limited to selected situations, locations, and patients. Reuse of facemasks and respirators is also common in some settings. Finally, some participants reported believing that the reuse of facemasks, particularly cloth masks, is safe, whereas others believed that the reuse of masks put staff at risk of infection. CONCLUSIONS: In low and middle-income countries, access to appropriate levels of personal protective equipment may be restricted owing to competing demands for funding in hospital settings. It is important that issues around reuse and extended use of medical masks/respirators and decontamination of cloth masks are addressed in policy documents to minimize the risk of infection.


Subject(s)
Attitude of Health Personnel , Cross Infection/prevention & control , Health Knowledge, Attitudes, Practice , Health Personnel , Masks/statistics & numerical data , Respiratory Tract Infections/prevention & control , Developing Countries , Female , Hospitals , Humans , Male , Vietnam
6.
Crit Care ; 17(6): R280, 2013 Dec 02.
Article in English | MEDLINE | ID: mdl-24295509

ABSTRACT

INTRODUCTION: Mortality from dengue infection is mostly due to shock. Among dengue patients with shock, approximately 30% have recurrent shock that requires a treatment change. Here, we report development of a clinical rule for use during a patient's first shock episode to predict a recurrent shock episode. METHODS: The study was conducted in Center for Preventive Medicine in Vinh Long province and the Children's Hospital No. 2 in Ho Chi Minh City, Vietnam. We included 444 dengue patients with shock, 126 of whom had recurrent shock (28%). Univariate and multivariate analyses and a preprocessing method were used to evaluate and select 14 clinical and laboratory signs recorded at shock onset. Five variables (admission day, purpura/ecchymosis, ascites/pleural effusion, blood platelet count and pulse pressure) were finally trained and validated by a 10-fold validation strategy with 10 times of repetition, using a logistic regression model. RESULTS: The results showed that shorter admission day (fewer days prior to admission), purpura/ecchymosis, ascites/pleural effusion, low platelet count and narrow pulse pressure were independently associated with recurrent shock. Our logistic prediction model was capable of predicting recurrent shock when compared to the null method (P < 0.05) and was not outperformed by other prediction models. Our final scoring rule provided relatively good accuracy (AUC, 0.73; sensitivity and specificity, 68%). Score points derived from the logistic prediction model revealed identical accuracy with AUCs at 0.73. Using a cutoff value greater than -154.5, our simple scoring rule showed a sensitivity of 68.3% and a specificity of 68.2%. CONCLUSIONS: Our simple clinical rule is not to replace clinical judgment, but to help clinicians predict recurrent shock during a patient's first dengue shock episode.


Subject(s)
Decision Support Techniques , Dengue/complications , Shock/etiology , Ascites/etiology , Ecchymosis/etiology , Hospitalization , Humans , Logistic Models , Platelet Count , Pleural Effusion/etiology , Pulse , Purpura/etiology , Recurrence , Shock/diagnosis , Time Factors
7.
Virol J ; 10: 280, 2013 Sep 11.
Article in English | MEDLINE | ID: mdl-24025733

ABSTRACT

BACKGROUND: In recent decades, Echovirus 30 (E30) and Japanese encephalitis virus (JEV) have been reported to be the common causative agents of acute meningitis among patients in South East Asia. An E30 outbreak in Vietnam in 2001-2002 gained our interest because the initial clinical diagnosis of infected patients was due to JEV infection. There are few clinical insights regarding E30 cases, and there are no reports comparing E30 and JEV acute meningitis/encephalitis cases based on clinical symptoms and case histories. We therefore aimed to identify reliable clinical methods to differentiate E30 and JEV acute meningitis/encephalitis. METHODS: A retrospective, cross-sectional study was conducted to compare E30 and JEV acute meningitis/encephalitis cases. We collected and analyzed the clinical records of 43 E30 confirmed cases (E30 group) and 60 JEV confirmed cases (JEV group). Clinical data were compared between the E30 and the JEV groups. Differences of clinical parameters were analyzed by certain statistical tests. RESULTS: Fever, headache, and vomiting were the most common symptoms in both the E30 and the JEV groups. Combined symptoms of headache and vomiting and the triad of symptoms of fever, headache, and vomiting were observed in more patients in the E30 group (E30 vs. JEV: 19% vs. 0%, p < 0.001; 74% vs. 27%, p < 0.001, respectively). On the other hand, strong neurological symptoms such as seizure (5% vs. 73%, p < 0.001) and altered consciousness (12% vs. 97%, p < 0.001) were manifested primarily in the JEV group. CSF leukocytosis was observed predominantly in the E30 group (80 vs. 18 cells/µL, p = 0.003), whereas decreasing CSF sugar level was observed predominantly in the JEV group (58.7 vs. 46.9 mg/dL, p < 0.001). CONCLUSION: Fever, headache, vomiting, absence of neurological symptoms (seizure, altered consciousness), and presence of CSF leukocytosis are important parameters to consider in differentiating E30 from JEV cases during early infection. Then, proper measures can be adopted immediately to prevent the spread of the disease in the affected areas.


Subject(s)
Clinical Medicine/methods , Encephalitis Virus, Japanese/isolation & purification , Encephalitis, Viral/diagnosis , Encephalitis, Viral/pathology , Enterovirus B, Human/isolation & purification , Meningitis, Viral/diagnosis , Meningitis, Viral/pathology , Adolescent , Cerebrospinal Fluid/cytology , Child , Child, Preschool , Cross-Sectional Studies , Diagnosis, Differential , Encephalitis, Viral/virology , Female , Headache/etiology , Humans , Infant , Leukocytosis/etiology , Male , Meningitis, Viral/virology , Nervous System Diseases/etiology , Retrospective Studies , Vietnam , Vomiting/etiology , Young Adult
8.
Virology ; 444(1-2): 337-42, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23891522

ABSTRACT

We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and NDiV-infected cells were investigated by electron microscopy 24-48 h after infection. The results show that the viral nucleocapsid-like particles form clusters concentrated in the vacuoles, the endoplasmic reticulum, and are scattered in the cytoplasm. Mature virions of NDiV were released as budding particles on the cell surface where viral components appear to lie beneath and along the plasma membrane. Free homogeneous virus particles were obtained by ultracentrifugation on sucrose gradients of culture fluids. The size of the round-shaped particles with a complete internal structure was 80 nm in diameter. This is the first study to provide information on the morphogenesis and ultrastructure of the first insect nidovirus NDiV, a missing evolutionary link in the emergence of the viruses with the largest RNA genomes.


Subject(s)
Nidovirales/isolation & purification , Nidovirales/ultrastructure , Animals , Cell Line , Cell Membrane/virology , Culicidae , Cytoplasm/virology , Microscopy, Electron , Nidovirales/physiology , Organelles/ultrastructure , Organelles/virology , Virion/ultrastructure , Virus Release
9.
PLoS Pathog ; 9(7): e1003500, 2013.
Article in English | MEDLINE | ID: mdl-23874204

ABSTRACT

The small size of RNA virus genomes (2-to-32 kb) has been attributed to high mutation rates during replication, which is thought to lack proof-reading. This paradigm is being revisited owing to the discovery of a 3'-to-5' exoribonuclease (ExoN) in nidoviruses, a monophyletic group of positive-stranded RNA viruses with a conserved genome architecture. ExoN, a homolog of canonical DNA proof-reading enzymes, is exclusively encoded by nidoviruses with genomes larger than 20 kb. All other known non-segmented RNA viruses have smaller genomes. Here we use evolutionary analyses to show that the two- to three-fold expansion of the nidovirus genome was accompanied by a large number of replacements in conserved proteins at a scale comparable to that in the Tree of Life. To unravel common evolutionary patterns in such genetically diverse viruses, we established the relation between genomic regions in nidoviruses in a sequence alignment-free manner. We exploited the conservation of the genome architecture to partition each genome into five non-overlapping regions: 5' untranslated region (UTR), open reading frame (ORF) 1a, ORF1b, 3'ORFs (encompassing the 3'-proximal ORFs), and 3' UTR. Each region was analyzed for its contribution to genome size change under different models. The non-linear model statistically outperformed the linear one and captured >92% of data variation. Accordingly, nidovirus genomes were concluded to have reached different points on an expansion trajectory dominated by consecutive increases of ORF1b, ORF1a, and 3'ORFs. Our findings indicate a unidirectional hierarchical relation between these genome regions, which are distinguished by their expression mechanism. In contrast, these regions cooperate bi-directionally on a functional level in the virus life cycle, in which they predominantly control genome replication, genome expression, and virus dissemination, respectively. Collectively, our findings suggest that genome architecture and the associated region-specific division of labor leave a footprint on genome expansion and may limit RNA genome size.


Subject(s)
Genome, Viral , Models, Biological , Phylogeny , RNA Viruses/genetics , 3' Untranslated Regions , 5' Untranslated Regions , Base Sequence , Conserved Sequence , Databases, Nucleic Acid , Exoribonucleases/chemistry , Exoribonucleases/genetics , Exoribonucleases/metabolism , Genome Size , Nidovirales/enzymology , Nidovirales/metabolism , Open Reading Frames , RNA Viruses/enzymology , Viral Proteins/chemistry , Viral Proteins/genetics , Viral Proteins/metabolism
10.
Arch Virol ; 158(11): 2273-84, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23728735

ABSTRACT

In this study, we isolated and characterized an insect nidovirus from the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae) in Vietnam, as an additional member of the new family Mesoniviridae in the order Nidovirales. The virus, designated "Dak Nong virus (DKNV)," shared many characteristics with Cavally virus and Nam Dinh virus, which have also been discovered recently in mosquitoes, and these viruses should be considered members of a single virus species, Alphamesonivirus 1. DKNV grew in cultured mosquito cells but could not replicate in the cultured vertebrate cells tested. N-terminal sequencing of the DKNV structural proteins revealed two posttranslational cleavage sites in the spike glycoprotein precursor. DKNV is assumed to be a new member of the species Alphamesonivirus 1, and the current study provides further understanding of viruses belonging to the new family Mesoniviridae.


Subject(s)
Culex/virology , Insect Viruses/classification , Insect Viruses/isolation & purification , Nidovirales/classification , Nidovirales/isolation & purification , Amino Acid Sequence , Animals , Base Sequence , Cell Line , Chlorocebus aethiops , Cricetinae , Female , Insect Viruses/genetics , Insect Viruses/growth & development , Molecular Sequence Data , Nidovirales/genetics , Nidovirales/growth & development , Phylogeny , Sequence Analysis, DNA , Vero Cells , Vietnam , Viral Proteins/chemistry , Viral Proteins/genetics , Viral Proteins/metabolism
11.
Am J Trop Med Hyg ; 88(4): 681-8, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23358634

ABSTRACT

Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Culex tritaeniorhynchus Giles collected in northern and southern Vietnam and two JEV strains were isolated from Culex vishnui Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their Cx. tritaeniorhynchus and Cx. vishnui.


Subject(s)
Culex/virology , Encephalitis Virus, Japanese/classification , Insect Vectors/virology , Amino Acid Sequence , Animals , Base Sequence , Cell Line , Encephalitis Virus, Japanese/isolation & purification , Encephalitis Virus, Japanese/pathogenicity , Encephalitis, Japanese/virology , Genes, Viral , Genetic Variation , Genotype , Membrane Glycoproteins/analysis , Membrane Glycoproteins/genetics , Phylogeny , RNA, Viral/genetics , Reverse Transcriptase Polymerase Chain Reaction , Vietnam , Viral Envelope Proteins/analysis , Viral Envelope Proteins/genetics
12.
Emerg Infect Dis ; 18(11): 1817-24, 2012 Nov.
Article in English | MEDLINE | ID: mdl-23092599

ABSTRACT

Since the end of the 1990s, unexplained outbreaks of acute encephalitis in children coinciding with litchi harvesting (May-July) have been documented in the Bac Giang Province in northern Vietnam. A retrospective ecologic analysis of data for 2004-2009 involving environmental, agronomic, and climatic factors was conducted to investigate the suspected association between the outbreaks and litchi harvesting. The clinical, biological, and immunologic characteristics of the patients suggested a viral etiology. The ecologic study revealed an independent association between litchi plantation surface proportion and acute encephalitis incidence: Incidence rate ratios were 1.52 (95% CI 0.90-2.57), 2.94 (95% CI 1.88-4.60), and 2.76 (95% CI 1.76-4.32) for second, third, and fourth quartiles, respectively, compared with the lowest quartile. This ecologic study confirmed the suspected association between incidence of acute encephalitis and litchi plantations and should be followed by other studies to identify the causative agent for this syndrome.


Subject(s)
Encephalitis/epidemiology , Encephalitis/etiology , Litchi/adverse effects , Acute Disease , Child , Child, Preschool , Female , Humans , Incidence , Male , Vietnam/epidemiology
13.
Arch Virol ; 157(8): 1623-8, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22527862

ABSTRACT

Recently, two independent surveillance studies in Côte d'Ivoire and Vietnam, respectively, led to the discovery of two mosquito-borne viruses, Cavally virus and Nam Dinh virus, with genome and proteome properties typical for viruses of the order Nidovirales. Using a state-of-the-art approach, we show that the two insect nidoviruses are (i) sufficiently different from other nidoviruses to represent a new virus family, and (ii) related to each other closely enough to be placed in the same virus species. We propose to name this new family Mesoniviridae. Meso is derived from the Greek word "mesos" (in English "in the middle") and refers to the distinctive genome size of these insect nidoviruses, which is intermediate between that of the families Arteriviridae and Coronaviridae, while ni is an abbreviation for "nido". A taxonomic proposal to establish the new family Mesoniviridae, genus Alphamesonivirus, and species Alphamesonivirus 1 has been approved for consideration by the Executive Committee of the ICTV.


Subject(s)
Culicidae/virology , Nidovirales/classification , RNA, Viral/analysis , Animals , Cote d'Ivoire , Nidovirales/genetics , Nidovirales/isolation & purification , Open Reading Frames , Phylogeny , RNA, Viral/genetics , Sequence Analysis, RNA , Vietnam
14.
Talanta ; 86: 271-7, 2011 Oct 30.
Article in English | MEDLINE | ID: mdl-22063541

ABSTRACT

In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1-10 µg/ml JEV antigens, with a detection limit of 0.75 µg/ml and that stable signals are measured in about 20 min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks.


Subject(s)
Antibodies, Immobilized/blood , Antibodies, Viral/blood , Antigens, Viral/analysis , Biosensing Techniques/methods , Antibodies, Immobilized/analysis , Antibodies, Immobilized/immunology , Antibodies, Viral/analysis , Antibodies, Viral/immunology , Encephalitis Virus, Japanese/immunology , Humans , Time Factors
15.
PLoS Pathog ; 7(9): e1002215, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21931546

ABSTRACT

Nidoviruses with large genomes (26.3-31.7 kb; 'large nidoviruses'), including Coronaviridae and Roniviridae, are the most complex positive-sense single-stranded RNA (ssRNA+) viruses. Based on genome size, they are far separated from all other ssRNA+ viruses (below 19.6 kb), including the distantly related Arteriviridae (12.7-15.7 kb; 'small nidoviruses'). Exceptionally for ssRNA+ viruses, large nidoviruses encode a 3'-5'exoribonuclease (ExoN) that was implicated in controlling RNA replication fidelity. Its acquisition may have given rise to the ancestor of large nidoviruses, a hypothesis for which we here provide evolutionary support using comparative genomics involving the newly discovered first insect-borne nidovirus. This Nam Dinh virus (NDiV), named after a Vietnamese province, was isolated from mosquitoes and is yet to be linked to any pathology. The genome of this enveloped 60-80 nm virus is 20,192 nt and has a nidovirus-like polycistronic organization including two large, partially overlapping open reading frames (ORF) 1a and 1b followed by several smaller 3'-proximal ORFs. Peptide sequencing assigned three virion proteins to ORFs 2a, 2b, and 3, which are expressed from two 3'-coterminal subgenomic RNAs. The NDiV ORF1a/ORF1b frameshifting signal and various replicative proteins were tentatively mapped to canonical positions in the nidovirus genome. They include six nidovirus-wide conserved replicase domains, as well as the ExoN and 2'-O-methyltransferase that are specific to large nidoviruses. NDiV ORF1b also encodes a putative N7-methyltransferase, identified in a subset of large nidoviruses, but not the uridylate-specific endonuclease that - in deviation from the current paradigm - is present exclusively in the currently known vertebrate nidoviruses. Rooted phylogenetic inference by Bayesian and Maximum Likelihood methods indicates that NDiV clusters with roniviruses and that its branch diverged from large nidoviruses early after they split from small nidoviruses. Together these characteristics identify NDiV as the prototype of a new nidovirus family and a missing link in the transition from small to large nidoviruses.


Subject(s)
Biological Evolution , Culex/virology , Nidovirales/classification , Nidovirales/genetics , RNA, Viral/genetics , Amino Acid Sequence , Animals , Base Sequence , Cell Line , Cloning, Molecular , Computational Biology , Exons , Exoribonucleases/genetics , Gene Expression Regulation, Viral , Genes, Viral , Genome Size , Molecular Sequence Data , Nidovirales/physiology , Nucleic Acid Conformation , Open Reading Frames/genetics , Phylogeny , Sequence Alignment , Sequence Analysis, DNA , Vietnam , Virus Replication
16.
J Virol ; 85(14): 6893-905, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21543488

ABSTRACT

Theiler's murine encephalitis viruses (TMEV) are divided into two subgroups based on their neurovirulence. Persistent strains resemble Theiler's original viruses (referred to as the TO subgroup), which largely induce a subclinical polioencephalomyelitis during the acute phase of the disease and can persist in the spinal cord of susceptible animals, inducing a chronic demyelinating disease. In contrast, members of the neurovirulent subgroup cause an acute encephalitis characterized by the rapid onset of paralysis and death within days following intracranial inoculation. We report herein the characterization of a novel neurovirulent strain of TMEV, identified using pyrosequencing technology and referred to as NIHE. Complete coverage of the NIHE viral genome was obtained, and it shares <90% nucleotide sequence identity to known TMEV strains irrespective of subgroup, with the greatest sequence variability being observed in genes encoding the leader and capsid proteins. The histopathological analysis of infected brain and spinal cord demonstrate inflammatory lesions and neuronal necrosis during acute infection with no evidence of viral persistence or chronic disease. Intriguingly, genetic analysis indicates the putative expression of the L protein, considered a hallmark of strains within the persistent subgroup. Thus, the identification and characterization of a novel neurovirulent TMEV strain sharing features previously associated with both subgroups will lead to a deeper understanding of the evolution of TMEV strains and new insights into the determinants of neurovirulence.


Subject(s)
Theilovirus/isolation & purification , Amino Acid Sequence , Animals , Brain/pathology , Brain/virology , Capsid/chemistry , Genome, Viral , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Phylogeny , Sequence Homology, Amino Acid , Spinal Cord/pathology , Spinal Cord/virology , Theilovirus/classification , Theilovirus/pathogenicity , Viral Tropism
17.
J Virol Methods ; 167(1): 31-6, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20304016

ABSTRACT

A rapid and comprehensive protocol, which combines simple purification and liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS), was developed for identification of arboviruses in infected culture fluid. Using this protocol, various arboviruses were detected including uncommon viruses that were described previously as Banna virus and Yunnan orbivirus. This approach is useful for the rapid screening of viral samples that cannot be identified by conventional gene amplification or immunological methods.


Subject(s)
Arboviruses/classification , Arboviruses/isolation & purification , Proteome/analysis , Proteomics/methods , Viral Proteins/analysis , Virology/methods , Aedes , Animals , Cell Line , Chlorocebus aethiops , Chromatography, Liquid , Spectrometry, Mass, Electrospray Ionization , Virus Cultivation
18.
J Gen Virol ; 90(Pt 4): 827-832, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19264633

ABSTRACT

The Japanese encephalitis virus (JEV) circulating in Japan consists of viruses with multiple phylogenetic origins. Phylogenetic analysis revealed that some JEV strains have recently migrated from south-east and continental east Asian countries. One phylogenetic subcluster of the JEV strains circulating in Japan was closely related to viruses isolated in Vietnam and China's inland region while other JEV subclusters were related to viruses isolated in Shanghai, China. One virus subcluster, however, was isolated solely in Japan and was not found in any other Asian country. Therefore, our data suggests that the JEVs that have remained or are circulating in Japan include a mixture of viruses that have previously migrated from south-east and continental east Asian countries.


Subject(s)
Culicidae/virology , Encephalitis Virus, Japanese , Encephalitis, Japanese , Insect Vectors/virology , Phylogeny , Animals , Asia, Southeastern/epidemiology , Cells, Cultured , Culicidae/physiology , Ecology , Encephalitis Virus, Japanese/classification , Encephalitis Virus, Japanese/genetics , Encephalitis Virus, Japanese/isolation & purification , Encephalitis Virus, Japanese/physiology , Encephalitis, Japanese/epidemiology , Encephalitis, Japanese/virology , Asia, Eastern/epidemiology , Genotype , Humans , Insect Vectors/physiology , Japan/epidemiology , Polymerase Chain Reaction , Sequence Analysis, DNA
19.
J Gen Virol ; 85(Pt 6): 1625-1631, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15166447

ABSTRACT

This study analyses the evolutionary relatedness of 16 Japanese encephalitis virus (JEV) isolates (nine from Vietnam and seven from Japan) to previously published JEV strains using E gene sequence data. Vietnamese and Japanese strains isolated between 1986 and 1990 were found to cluster in genotype 3. However, more recent Vietnamese and Japanese strains isolated between 1995 and 2002 grouped within genotype 1, now a dominant though previously unreported genotype in Vietnam. In addition, in this study, strains isolated between 1995 and 2002 were more closely related to those isolated in the 1990s than to the older genotype 1 strains. Recently, the introduction of JEV genotype 1 into Japan and Korea has also been reported. Hence this genotype shift phenomenon may be occurring throughout all East Asia. Further studies on JEV ecology are needed to clarify the mechanism of JEV genotype 1 spread to new territories.


Subject(s)
Encephalitis Virus, Japanese/classification , Animals , Asia, Southeastern , Encephalitis Virus, Japanese/genetics , Encephalitis Virus, Japanese/isolation & purification , Asia, Eastern , Genotype , Humans , Phylogeny , Vietnam
SELECTION OF CITATIONS
SEARCH DETAIL
...