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1.
Pan Afr Med J ; 47: 175, 2024.
Article in English | MEDLINE | ID: mdl-39036016

ABSTRACT

Introduction: in areas with intense perennial malaria transmission, limited data is available on the impact of environmental conditions especially rainfall on naturally acquired immunity against promising malaria vaccine candidates. For this reason, we have compared IgG antibody responses specific to Plasmodium spp. derived MSP3 and UB05 vaccine candidates, in plasma of children living in two areas of Cameroon differing in rainfall conditions. Methods: data about children less than 5 years old was collected during the years 2017 and 2018. Next malaria asymptomatic P. falciparum (Pf) infected children were selected following malaria test confirmation. MSP3 and UB05 specific IgG antibody responses were measured in participant´s plasma using enzyme-linked immunosorbent assay (ELISA). Results: interestingly, IgG antibody responses specific to UB05 were significantly higher (p<0.0001) in Pf-negative children when compared to their asymptomatic Pf-infected counterparts living in monomodal rainfall areas. In contrast, a significantly higher (p<0.0001) IgG response to MSP3 was observed instead in asymptomatic Pf-infected children in the same population. In addition, IgG responses specific to UB05 remained significantly higher in bimodal when compared to monomodal rainfall areas irrespective of children´s Pf infection status (p<0.0055 for Pf-positive and p<0.0001 for negative children). On the contrary, IgG antibody responses specific to MSP3 were significantly higher in bimodal relative to monomodal rainfall areas (P<0.0001) just for Pf-negative children. Conclusion: thus IgG antibody responses specific to UBO5 are a better correlate of naturally acquired immunity against malaria in Pf-negative Cameroonian children especially in monomodal rainfall areas.


Subject(s)
Antibodies, Protozoan , Antigens, Protozoan , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G , Malaria, Falciparum , Plasmodium falciparum , Protozoan Proteins , Humans , Cameroon , Malaria, Falciparum/immunology , Malaria, Falciparum/epidemiology , Immunoglobulin G/blood , Child, Preschool , Plasmodium falciparum/immunology , Protozoan Proteins/immunology , Antigens, Protozoan/immunology , Antibodies, Protozoan/blood , Infant , Female , Malaria Vaccines/administration & dosage , Malaria Vaccines/immunology , Male , Rain , Recombinant Proteins/immunology
2.
Sex Transm Dis ; 50(11): e30-e33, 2023 11 01.
Article in English | MEDLINE | ID: mdl-37732834

ABSTRACT

ABSTRACT: The contribution of chlamydia to secondary infertility in women is poorly understood. Among 404 female participants enrolled in a previous study in Cameroon, 142 had secondary infertility (cases) and 262 were pregnant with no history of infertility (controls) , Chlamydia trachomatis seropositivity was 92%. Seropositivity did not significantly differ by case/control status.


Subject(s)
Chlamydia Infections , Infertility, Female , Pregnancy , Female , Humans , Chlamydia trachomatis , Chlamydia Infections/complications , Chlamydia Infections/epidemiology , Cameroon/epidemiology , Antibody Formation , Antibodies, Bacterial
3.
Phytochemistry ; 83: 95-103, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22963707

ABSTRACT

Three compounds, ficusamide (1), ficusoside (2) and elasticoside (3), were isolated from the bark of aerial roots of Ficus elastica (Moraceae), together with nine known compounds, including four triterpenes, three steroids and two aliphatic linear alcohols. The chemical structures of the three compounds were established by extensive 1D and 2D NMR spectroscopy, mass spectrometry and by comparison with published data. The growth inhibitory effect of the crude extract and isolated compounds was evaluated against several microorganisms and fungi. The cytotoxicity against human cancer cell lines was also assessed. Ficusamide (1) displayed a moderate in vitro growth inhibitory activity against the human A549 lung cancer cell line and a strong activity against Staphylococcus saprophyticus, while elasticoside (3) showed a potent activity on Enterococcus faecalis.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Enterococcus faecalis/drug effects , Moraceae/chemistry , Saponins/pharmacology , Staphylococcus saprophyticus/drug effects , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Line, Tumor , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Microbial Sensitivity Tests , Molecular Conformation , Plant Components, Aerial/chemistry , Plant Roots/chemistry , Saponins/chemistry , Saponins/isolation & purification , Structure-Activity Relationship
4.
J Ethnopharmacol ; 128(2): 476-81, 2010 Mar 24.
Article in English | MEDLINE | ID: mdl-20093175

ABSTRACT

AIM OF THE STUDY: Seven extracts and eight compounds from four selected Cameroonian medicinal plants, Solanecio mannii Hook f. (Asteraceae), Monodora myristica Dunal (Annonaceae), Albizia gummifera (J.F. Gmel) C.A. Smith (Fabaceae/Mimosoideae) and Glyphaea brevis (Spreng) Monachino (Tiliaceae), traditionally used for the treatment of hepatitis, parasites and other infectious diseases, were tested in vitro for their antimicrobial activity against Gram-positive (5 species) and Gram-negative (4 species) bacteria species and pathogenic yeasts (2 Candida species), to establish whether or not they have antimicrobial activity and to validate scientifically their use in traditional medicine. MATERIALS AND METHODS: The agar disc diffusion and the microbroth dilution methods were used to determine the zone of inhibition between the edge of the filter paper and the edge of the inhibition area (IZ) and the minimal inhibitory concentration (MIC) respectively. RESULTS: The most active extracts against Candida albicans and Candida krusei were respectively the cyclohexane extract from the fruits of Monodora myristica and the ethyl acetate extract from the stem bark of Albizia gummifera (MIC=6.3 microg/ml for both extracts). The lowest MIC value (1.6 microg/ml) for purified compounds was obtained on Candida albicans with a mixture of linear aliphatic primary alcohols (n-C24H50O to n-C30H62O), with n-hexacosanol (1b) as major compound and mixture of fatty acid esters of diunsaturated linear 1,2-diols (6). CONCLUSION: These results afford ground informations for the potential use of the crude extracts of these species as well as of some of the isolated compounds in bacterial and fungal infections.


Subject(s)
Anti-Infective Agents/pharmacology , Bacteria/drug effects , Mycoses/drug therapy , Plants, Medicinal/chemistry , Yeasts/drug effects , Albizzia , Annonaceae , Anti-Infective Agents/isolation & purification , Cameroon , Candida/drug effects , Candida albicans/drug effects , Dosage Forms , Fatty Alcohols/chemistry , Medicine, Traditional , Microbial Sensitivity Tests/methods , Tiliaceae
5.
Nat Prod Res ; 22(4): 296-304, 2008 Mar 10.
Article in English | MEDLINE | ID: mdl-18322845

ABSTRACT

Spathoside, a new cerebroside was isolated from the stem bark of Spathodea campanulata, besides known compounds (n-alkanes, linear aliphatic alcohols, sitosterol and their esters, beta-sitosterol-3-O-beta-D-glucopyranoside, oleanolic acid, pomolic acid, p-hydroxybenzoic acid and phenylethanol esters). The structures of the isolated compounds were established by spectroscopic studies. The antibacterial activity of the isolated compounds against a wide range of microorganisms was examined. They inhibited significantly the growth of some gram-positive and -negative bacteria.


Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bignoniaceae/chemistry , Cerebrosides/chemistry , Plant Bark/chemistry , Bacteria/drug effects , Cerebrosides/pharmacology , Molecular Structure , Plant Stems/chemistry
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