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1.
Avian Pathol ; 49(1): 5-14, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31259607

ABSTRACT

Since the first report of chicken infectious anaemia virus (CIAV) in Vietnam in 2013, there have not been many studies focused on the detection of CIAV or the molecular characteristics of the virus. This study attempted to investigate the presence of CIAV in northern Vietnam by molecular-based methods. Regarding the spatial distribution of CIAV, the PCR-based results showed that CIAV was detected in 47 out of 64 farms (73.4%) and in all 10 investigated provinces. Of the 119 samples assayed by PCR, 74 (62.2%) tested positive for CIAV DNA. By arranging the samples into different categories, it was found that CIAV was detected at high rates (above 50%) based on all 4 evaluated criteria as follows: production type of chicken, housing system, flock size and age group. Different housing systems were significantly associated with the detection rates of CIAV (P = 0.003). By genetic analyses, all of the Vietnamese CIAVs were found to (i) lack substitutions related to attenuation substitutions, (ii) group separately from vaccine-like CIAVs and (iii) belong to genogroups G2 and G3 of CIAV. Because of the wide distribution of CIAV and because the virus was confirmed not to be vaccine-like viruses, it is suggested that further studies be conducted on the clinical form of chicken infectious anaemia, as well as the immunosuppressive effect of CIAV on chickens in Vietnam.RESEARCH HIGHLIGHTS Wide distribution of chicken infectious anaemia virus (CIAV) in northern Vietnam.Vietnamese CIAVs belong to genogroups G2 and G3 of CIAV.


Subject(s)
Chicken anemia virus/genetics , Chickens , Circoviridae Infections/veterinary , Poultry Diseases/epidemiology , Poultry Diseases/virology , Age Factors , Analysis of Variance , Animals , Chicken anemia virus/classification , Chicken anemia virus/immunology , Chickens/classification , Circoviridae Infections/epidemiology , Circoviridae Infections/virology , DNA, Viral/chemistry , DNA, Viral/isolation & purification , Genome, Viral , Housing, Animal , Immune Tolerance , Phylogeny , Polymerase Chain Reaction/veterinary , Population Density , Vietnam/epidemiology
2.
J Microbiol Biotechnol ; 22(5): 699-707, 2012 May.
Article in English | MEDLINE | ID: mdl-22561866

ABSTRACT

Since the 2009 pandemic human H1N1 influenza A virus emerged in April 2009, novel reassortant strains have been identified throughout the world. This paper describes the detection and isolation of reassortant strains associated with human pandemic influenza H1N1 and swine influenza H1N2 (SIV) viruses in swine populations in South Korea. Two influenza H1N2 reassortants were detected, and subtyped by PCR. The strains were isolated using Madin- Darby canine kidney (MDCK) cells, and genetically characterized by phylogenetic analysis for genetic diversity. They consisted of human, avian, and swine virus genes that were originated from the 2009 pandemic H1N1 virus and a neuraminidase (NA) gene from H1N2 SIV previously isolated in North America. This identification of reassortment events in swine farms raises concern that reassortant strains may continuously circulate within swine populations, calling for the further study and surveillance of pandemic H1N1 among swine.


Subject(s)
Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza A Virus, H1N2 Subtype/isolation & purification , Influenza, Human/virology , Orthomyxoviridae Infections/veterinary , Reassortant Viruses/isolation & purification , Swine Diseases/virology , Animals , Cell Line , Dogs , Humans , Influenza A Virus, H1N1 Subtype/classification , Influenza A Virus, H1N1 Subtype/genetics , Influenza A Virus, H1N2 Subtype/classification , Influenza A Virus, H1N2 Subtype/genetics , Influenza, Human/epidemiology , Molecular Sequence Data , Orthomyxoviridae Infections/virology , Phylogeny , Reassortant Viruses/classification , Reassortant Viruses/genetics , Republic of Korea/epidemiology , Swine , Swine Diseases/epidemiology , Viral Proteins/genetics , Viral Proteins/metabolism
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