ABSTRACT
Perfluorohexane gas lowers the kinetic barrier that opposes the displacement of albumin by dipalmitoylphosphatidylcholine at the air/water interface submitted to sinusoidal oscillations at frequencies in the range of those encountered in respiration.
Subject(s)
Fluorocarbons/chemistry , Pulmonary Surfactants/chemistry , Serum Albumin, Bovine/chemistry , 1,2-Dipalmitoylphosphatidylcholine/chemistry , Adsorption , Air , Animals , Cattle , Fluorocarbons/metabolism , Gases/chemistry , Kinetics , Liposomes/chemistry , Liposomes/metabolism , Microscopy, Fluorescence , Pulmonary Surfactants/metabolism , Serum Albumin, Bovine/metabolism , Water/chemistryABSTRACT
The adsorption dynamics of a series of phospholipids (PLs) at the interface between an aqueous solution or dispersion of the PL and a gas phase containing the nonpolar, nonamphiphilic linear perfluorocarbon perfluorohexane (PFH) was studied by bubble profile analysis tensiometry. The PLs investigated were dioctanoylphosphatidylcholine (DiC(8)-PC), dilaurylphosphatidylcholine, dimyristoylphosphatidylcholine, and dipalmitoylphosphatidylcholine. The gas phase consisted of air or air saturated with PFH. The perfluorocarbon gas was found to have an unexpected, strong effect on both the adsorption rate and the equilibrium interfacial tension (γ(eq)) of the PLs. First, for all of the PLs, and at all concentrations investigated, the γ(eq) values were significantly lower (by up to 10 mN m(-1)) when PFH was present in the gas phase. The efficacy of PFH in decreasing γ(eq) depends on the ability of PLs to form micelles or vesicles in water. For vesicles, it also depends on the gel or fluid state of the membranes. Second, the adsorption rates of all the PLs at the interface (as assessed by the time required for the initial interfacial tension to be reduced by 30%) are significantly accelerated (by up to fivefold) by the presence of PFH for the lower PL concentrations. Both the surface-tension reducing effect and the adsorption rate increasing effect establish that PFH has a strong interaction with the PL monolayer and acts as a cosurfactant at the interface, despite the absence of any amphiphilic character. Fitting the adsorption profiles of DiC(8)-PC at the PFH-saturated air/aqueous solution interface with the modified Frumkin model indicated that the PFH molecule lay horizontally at the interface.
