Stable isotope dilution assay and HS-SPME-GCMS quantification of key aroma volatiles of Australian pineapple (Ananas comosus) cultivars.

Pineapple aroma is one of the most important sensory quality traits that influences consumer purchasing patterns. Reported in this paper is a high throughput method to quantify in a single analysis the key volatile organic compounds that contribute to the aroma of pineapple cultivars grown in Australia. The method constituted stable isotope dilution analysis in conjunction with headspace solid-phase microextraction coupled with gas-chromatography mass spectrometry. Deuterium labelled analogues of the target analytes purchased commercially were used as internal standards. Twenty-six volatile organic compounds were targeted for quantification and the resulting calibration functions of the matrix -matched validated method had determination coefficients (R2) ranging from 0.9772 to 0.9999. The method was applied to identify the key aroma volatile compounds produced by popular pineapple cultivars such as 'Aus Carnival', 'Aus Festival', 'Aus Jubilee', 'Aus Smooth (Smooth Cayenne)' and 'Aussie Gold (73-50)', grown in Queensland, Australia. Pineapple cultivars varied in its content and composition of free volatile components, which were predominantly comprised of esters, followed by terpenes, alcohols, aldehydes, and ketones.

Epilepsy self-management mobile health application: A needs assessment in people with epilepsy and caregivers in Viet Nam.

OBJECTIVES: This study aimed to determine (1) the needsof Vietnamese people with epilepsy (PWE) and their caregivers for self-management mobile health applications and (2) the self-management features expected to be included in an application. METHODS: The survey consisted of an anonymous self-administered questionnaire that was distributed to PWE and caregivers from the age of 18 in Vietnam through online platforms and onsite at Nguyen Tri Phuong Hospital and University Medical Center, Ho Chi Minh City, from February 2022 to May 2022. The questionnaire assessed the participants' attitudes toward epilepsy self-management mobile applications, their willingness to use applications, and their expectations of the contents of an application. RESULTS: Responses from 103 participants were submitted. Eighty-one participants (78.6%) reported using a smartphone, but only 50.6% of those claimed to know about self-management applications. Most respondents (70.9%) thought the applications would be useful for disease self-management, and 68.9% were willing to use epilepsy self-management applications. In addition, the most expected features to be included in self-management applications were epilepsy information, seizure first aid, connecting with medical professionals, and a seizure diary. CONCLUSION: Most Vietnamese PWE and caregivers had a willingness to use epilepsy self-management applications.The expected features are related to all aspects of self-management, including information, seizure, medication, and safety management.

Avian influenza A(H5) virus circulation in live bird markets in Vietnam, 2017-2022.

BACKGROUND: Highly pathogenic avian influenza A(H5) human infections are a global concern, with many A(H5) human cases detected in Vietnam, including a case in October 2022. Using avian influenza virus surveillance from March 2017-September 2022, we described the percent of pooled samples that were positive for avian influenza A, A(H5), A(H5N1), A(H5N6), and A(H5N8) viruses in live bird markets (LBMs) in Vietnam. METHODS: Monthly at each LBM, 30 poultry oropharyngeal swab specimens and five environmental samples were collected. Samples were pooled in groups of five and tested for influenza A, A(H5), A(H5N1), A(H5N6), and A(H5N8) viruses by real-time reverse-transcription polymerase chain reaction. Trends in the percent of pooled samples that were positive for avian influenza were summarized by LBM characteristics and time and compared with the number of passively detected avian influenza outbreaks using Spearman's rank correlation. RESULTS: A total of 25,774 pooled samples were collected through active surveillance at 167 LBMs in 24 provinces; 36.9% of pooled samples were positive for influenza A, 3.6% A(H5), 1.9% A(H5N1), 1.1% A(H5N6), and 0.2% A(H5N8). Influenza A(H5) viruses were identified January-December and at least once in 91.7% of sampled provinces. In 246 A(H5) outbreaks in poultry; 20.3% were influenza A(H5N1), 60.2% A(H5N6), and 19.5% A(H5N8); outbreaks did not correlate with active surveillance. CONCLUSIONS: In Vietnam, influenza A(H5) viruses were detected by active surveillance in LBMs year-round and in most provinces sampled. In addition to outbreak reporting, active surveillance for A(H5) viruses in settings with high potential for animal-to-human spillover can provide situational awareness.

Engineered therapeutic proteins for sustained-release drug delivery systems.

Proteins play a vital role in diverse biological processes in the human body, and protein therapeutics have been applied to treat different diseases such as cancers, genetic disorders, autoimmunity, and inflammation. Protein therapeutics have demonstrated their advantages, such as specific pharmaceutical effects, low toxicity, and strong solubility. However, several disadvantages arise in clinical applications, including short half-life, immunogenicity, and low permeation, leading to reduced drug effectiveness. The structure of protein therapeutics can be modified to increase molecular size, leading to prolonged stability and increased plasma half-life. Notably, the controlled-release delivery systems for the sustained release of protein drugs and preserving the stability of cargo proteins are envisioned as a potential approach to overcome these challenges. In this review, we summarize recent research progress related to structural modifications (PEGylation, glycosylation, poly amino acid modification, and molecular biology-based strategies) and promising long-term delivery systems, such as polymer-based systems (injectable gel/implants, microparticles, nanoparticles, micro/nanogels, functional polymers), lipid-based systems (liposomes, solid lipid nanoparticles, nanostructured lipid carriers), and inorganic nanoparticles exploited for protein therapeutics. STATEMENT OF SIGNIFICANCE: In this review, we highlight recent advances concerning modifying proteins directly to enhance their stability and functionality and discuss state-of-the-art methods for the delivery and controlled long-term release of active protein therapeutics to their target site. In terms of drug modifications, four widely used strategies, including PEGylation, poly amino acid modification, glycosylation, and genetic, are discussed. As for drug delivery systems, we emphasize recent progress relating to polymer-based systems, lipid-based systems developed, and inorganic nanoparticles for protein sustained-release delivery. This review points out the areas requiring focused research attention before the full potential of protein therapeutics for human health and disease can be realized.

Review of the Aroma Chemistry of Pineapple (Ananas comosus).

Pineapple (Ananas comosus), one of the most flavorful and popular tropical fruits consumed worldwide, is known to contain many volatile organic compounds (VOCs) at varying concentrations. Much attention has been paid to understand which VOC plays a significant role in the sensory aroma notes of the fruit. Though, nearly 480 VOCs have been identified to date using different analytical techniques, only 40 compounds are reported to contribute to the unique flavor of pineapple. A consolidated database of the reported VOCs and key aroma compounds of pineapple is currently not available. This review discusses the available published data regarding the analytical methodologies, volatile profile of different varieties of pineapple at different maturities, and their characteristic aroma compounds. The output of this review is a subset of key pineapple aroma volatiles that can be targeted in analytical method development for utilization in varietal improvement or other research of pineapple.

Morphological comparison of the cranial movement apparatus in mudskippers (Gobiidae: Oxudercinae).

Possession of the neck allows vertebrates to move the head independently from the trunk. Fish do not have the neck and thus the cranial mobility could be limited. Oxudercine gobies show full range of habitat transition from aquatic to terrestrial environments and exhibit flexible cranial movement, yet the cranium-movement apparatus is little known. In this study, we investigated the anatomy of the structure of the eight oxudercine gobies, Oxuderces nexipinnis, Parapocryptes serperaster, Pseudapocryptes elongatus, Scartelaos histophorus, Boleophthalmus boddarti, Periophthalmus chrysospilos, Periophthalmodon schlosseri, and Periophthalmodon septemradiatus. These species share similarities in the specialized features of the craniovertebral joint and the epaxials attaching onto different locations of the neurocranium. On the other hand, large space between the ventral portions of the craniovertebral joint only occurs in O. nexipinnis, Pd. elongatus, Pn. schlosseri and Pn. septemradiatus. Hypaxials are hypertrophied at the insertion point and attach more anteriorly onto the ventral side of the neurocranium in B. boddarti, O. nexipinnis, Pa. serperaster, Pd. elongatus, and S. histophorus, whereas the muscles are small and attach posteriorly in the remaining species. There were significant differences in the area occupancy ratio of the post-cranial neural spines, the lever arm ratio of the cranial rotation, and the angle between the horizontal plane and the plane through the craniovertebral joint among the species. The cranial depression presumably facilitates grazing of oxudercine gobies in their early stage of terrestrial transition, whereas the cranial elevation parameters are contradictory to the terrestrial gradient. The cranium-movement morphometrics partially agree with the phylogeny.

Estimation of rooting depth of 137Cs uptake by plants.

Understanding the soil-to-plant transfer process of 137Cs is essential for predicting the contamination levels of plants in contaminated areas. The rooting depth is considered one of the key factors explaining the difference in the activity concentration of 137Cs in different plant species. In this study, the distributions of 137Cs and 133Cs in soils and plants were investigated, and the plants' rooting depth of 137Cs uptake was estimated using the 137Cs/133Cs ratios in exchangeable fractions of soils and biological samples. The results showed that different plant species accumulate different levels of 137Cs and 133Cs. The 137Cs/133Cs ratios were fairly constant in plants of the same species. The average 137Cs/133Cs ratios in bamboo grasses and ferns were 0.015 ± 0.009 (n = 5) and 0.13 ± 0.04 Bq ng-1 (n = 10) in Yamakiya, respectively. The percentage of 137Cs in the exchangeable fraction of the uppermost soil layer was lower than that in the deeper soil layers. The activity concentrations of 137Cs in the soil profiles decreased sharply with depth, whereas the depth distributions of 133Cs were uniform. Therefore, the 137Cs/133Cs ratios were driven mainly by the 137Cs activity concentrations in soil. The plants' rooting depths of 137Cs uptake were estimated on the basis of the relationships between the averaged 137Cs/133Cs ratio in the soil layer and the 137Cs/133Cs ratio in the plant. The results indicate that the deeper-rooted species such as bamboo grasses have a lower accumulation of 137Cs than the superficial-rooting species such as ferns. The soil-to-plant transfer factors would be determined using rooting depth by calculating the averaged activity concentration of 137Cs within the estimated rooting depth.

Effect of processing on the solubility and molecular size of oat ß-glucan and consequences for starch digestibility of oat-fortified noodles.

White wheat salted noodles containing oats have a slower digestion rate those without oats, with potential health benefits. Oat ß-glucan may play an important role in this. Effects of sheeting and shearing during noodle-making and subsequent cooking on ß-glucan concentration, solubility, molecular size and starch digestibility were investigated. The levels of ß-glucan were reduced by 16% after cooking, due to the loss of ß-glucan into the cooking water. Both the noodle-making process and cooking increased the solubility of ß-glucan but did not change its average molecular size. Digestion profiles show that ß-glucan in wholemeal oat flour did not change starch digestion rates compared with isolated starch, but reduced the starch digestion rate of oat-fortified wheat noodles compared to the control (wheat noodles). Confocal laser scanning microscopy suggests that interaction between ß-glucan and protein contributes to the starch-protein matrix and changes noodle microstructure, and thus alters their digestibility.

Influences of Agrochemicals on Health and Ecology in Vietnamese Mango Cultivation.

The study aims to identify risks of agrochemicals that impact farmworkers, consumers, and ecology in Vietnamese mango cultivation to enhance safety and friendly production. The study finds out the total numbers of root fertilizers (N-P-K) of the noncooperative and cooperative farmers are similar, approximately 1,400 kg/ha/year higher than those in other countries. Excessive fertilizer usage is a potential threat to soil, water, and air pollution. In addition, the findings indicate that the ecology component is undergoing the most negative impact from excessive agrochemical use in mango farming. The vast majority of agrochemicals in mango cultivation are fungicide and paclobutrazol over 90% of the total number of agrochemicals used in both noncooperative and cooperative farmer groups among the three seasons. Total field EIQ of the cooperative grower category is less than that of the noncooperative grower category. These results show that mango cultivation should consider rejecting the banned active ingredients of glyphosate, paraquat, and carbendazim as well as reducing fungicide and paclobutrazol usage and encouraging cooperative participation to safeguard the environment and human health. Moreover, science information needs to be closely linked and fed back to policy development to boost the management of the awareness of the ecological risks for farmers associated with reducing agrochemical use in mango cultivation.

Burden of Influenza-Associated Respiratory Hospitalizations, Vietnam, 2014-2016.

Influenza burden estimates are essential to informing prevention and control policies. To complement recent influenza vaccine production capacity in Vietnam, we used acute respiratory infection (ARI) hospitalization data, severe acute respiratory infection (SARI) surveillance data, and provincial population data from 4 provinces representing Vietnam's major regions during 2014-2016 to calculate provincial and national influenza-associated ARI and SARI hospitalization rates. We determined the proportion of ARI admissions meeting the World Health Organization SARI case definition through medical record review. The mean influenza-associated hospitalization rates per 100,000 population were 218 (95% uncertainty interval [UI] 197-238) for ARI and 134 (95% UI 119-149) for SARI. Influenza-associated SARI hospitalization rates per 100,000 population were highest among children <5 years of age (1,123; 95% UI 946-1,301) and adults >65 years of age (207; 95% UI 186-227), underscoring the need for prevention and control measures, such as vaccination, in these at-risk populations.

Starch molecular fine structure is associated with protein composition in chickpea seed.

Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein. This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties. It is found that storage proteins and starch biosynthetic enzymes influence each other. The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content. In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000). This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.

Optimizing lime pretreatment of rice straw for biolipid production using oleaginous microorganisms.

This work focuses on the lime pretreatment of rice straw, agricultural waste in northeast Vietnam, for microbial lipid (bio-lipid) production. A response surface methodology approach was performed to optimize parameters for the pretreatment process. These parameters are the concentration of Ca(OH)2, hydrolysis temperature, and maintained time; the effect is considered bio-lipid production yield. The lipid yield was estimated through consolidated bioprocessing of the pretreated rice straw as a substrate and using the fungus strain (Aspergillus oryzae 32) and the yeast strain (Lipomyces starkeyi 22). As a result, the optimal pretreatment conditions for maximum lipid yield were obtained at Ca(OH)2 concentration of 12 g/L and hydrolysis temperature of 110 °C within 60 min. The accumulated lipid in fermentation using these oleaginous microorganisms was 8.5g/100g oven-dry weight of rice straw (10.9g/100g for pretreated rice straw). The biolipid consisted of 42.6% saturated, 21.1% monounsaturated (MUFA), and 35.1% polyunsaturated (PUFA) fatty acids.

A case study of an influenza vaccination program for health care workers in Vietnam.

BACKGROUND: In 2017, the Vietnam Ministry of Health conducted a demonstration project to introduce seasonal influenza vaccination to health care workers. A total of 11,000 doses of influenza vaccine, single-dose prefilled syringes, were provided free to HCWs at 29 selected hospitals, clinics, and research institutes in four provinces: Hanoi, Khanh Hoa, Dak Lak and Ho Chi Minh City. METHODS: Before the campaign, a workshop was organized to discuss an implementation plan including technical requirements, cold chain, uptake reporting, and surveillance for adverse events following immunization. All sites distributed communication materials and encouraged their staff to register for vaccination. Following immunization sessions, sites sent reports on uptake and adverse events following immunization. Left-over vaccine was transferred to other sites to maximize vaccine use. RESULTS: The average uptake was 57% for all health care workers, with 11 sites achieving 90% and above. These 11 sites were small with less than 500 staff, including 5 primary hospitals, 3 preventive medicine units, and 2 referral hospitals. Among the six biggest sites with over 1000 staff, four sites had the lowest uptake (14-47%). Most of the high-uptake sites were from the central to the south; only one site, a referral hospital, was from the north. After redistribution of left-over vaccine, only 130 vaccine doses (1.2%) were not used and destroyed. Based on factors that affected uptake, including registration levels, differing communication strategies, availability of vaccination, and commitment by health facility leaders, we recommended ways to increase health care worker coverage; recommendations to improve reporting adverse events following immunization were also made. CONCLUSIONS: The project demonstrated that it was feasible to conduct influenza vaccination campaigns among health care workers in Vietnam. Improvements in promotion of registration, more intense pre-planning, especially at larger facilities, and wider, more consistent availability of communication materials will result in increased efficiency and coverage in this program's future expansion.

The contribution of ß-glucan and starch fine structure to texture of oat-fortified wheat noodles.

Wheat flour noodles are sometimes fortified with ß-glucan for nutritional value, but this can decrease eating quality. The contributions of ß-glucan and starch molecular fine structure to physicochemical properties of wholemeal oat flour and to the texture of oat-fortified white salted noodles were investigated here. Hardness of oat-fortified noodles was controlled by the longer amylopectin chains (DP ≥ 26) and amount of longer amylose chains (DP ≥ 1000). Higher levels of ß-glucan, in the range from 3.1 to 5.2%, result in increased noodle hardness. Pasting viscosities of wholemeal oat flour positively correlate with the hardness of oat-fortified noodles. The swelling power of oat flour is not correlated with either pasting viscosities of oat flour or noodle hardness. Longer amylopectin chains and the amount of longer amylose chains both control the pasting viscosities of oat flour, which in turn affect noodle texture. This provides new means, based on starch and ß-glucan molecular structure, to choose oats with optimal starch structure and ß-glucan content for targeted oat-fortified noodle quality.

The catalytic effects of sulfur in ethane dehydroaromatization.

In this work, we investigated the catalytic effect of adding sulfur on Zn/ZSM-5 catalyst for direct conversion of ethane to aromatics. We show that the continuous addition of hydrogen sulfide (H2S) effectively stabilizes zinc, prevents coking and results in a highly selective and stable catalyst. Considering the high content of sulfur in shale gas resources, these results highlight the importance of investigating catalysts under realistic operating conditions.

Acceptability of seasonal influenza vaccines among health care workers in Vietnam in 2017.

INTRODUCTION: A demonstration project in Vietnam provided 11,000 doses of human seasonal influenza vaccine free of charge to healthcare workers (HCWs) in 4 provinces of Vietnam. Through this project, we conducted an acceptability survey to identify the main reasons that individuals chose to be vaccinated or not to inform and improve future immunization activities. METHODS: We conducted a descriptive cross-sectional survey from May to August 2017 among HCWs at 13 selected health facilities. We employed logistic regression to determine the association between demographic and professional factors, and the decision to receive seasonal influenza vaccine. We performed post-hoc pairwise comparisons among reasons for and against vaccination using Chi square and Fisher's exact tests (for cell sizes <5). RESULTS: A total of 1,450 HCWs participated in the survey, with a higher proportion of females than males (74% versus 26%). The median age of the participating HCWs was 35 years (median range 25.8-44.2). Among those surveyed, 700 (48%) HCWs were vaccinated against seasonal influenza during the first half of 2017. Younger HCWs under 30 and 30-39 years old were less likely to get vaccinated against seasonal influenza than HCWs ≥50 years old (OR = 0.5; 95%CI 0.4-0.8 and OR = 0.6; 95%CI 0.4-0.8 respectively). Nurses and other employees were more likely to get seasonal influenza vaccination than physicians (OR = 1.5; 95%CI 1.0-2.4 and OR = 2.0; 95%CI 1.2-3.2 respectively). The most common reason for accepting vaccination was fear of getting influenza (66%) and the most common reason for not getting vaccinated was concern about vaccine side effects (23%). CONCLUSION: Acceptability of seasonal influenza vaccines in this setting varied among HCWs by age group and job category. Interventions to increase acceptance of vaccine among HCWs in this setting where influenza vaccine is being introduced free for the first time should include targeted risk communication on vaccine safety and efficacy.

An optimal control approach to design entire relaxation dispersion experiments.

A general approach is introduced to optimize experiments for the analysis of spin systems in the presence of chemical exchange. Rather than optimizing individual pulse sequence elements, such as refocusing pulses, entire relaxation dispersion sequences are optimized in the form of a single shaped pulse. This is achieved by defining a performance index that is only based on the remaining signal after the relaxation dispersion sequence for a range of exchange, relaxation, offset, and rf inhomogeneity parameters. The approach is demonstrated by optimizing energy-limited broadband relaxation dispersion sequences that closely approach the overall effect of ideal CPMG sequences. As illustrated both theoretically and experimentally, significant improvements are found compared to standard amplitude or energy-limited CPMG sequences.

Effectiveness of Periodic Treatment of Quercetin against Influenza A Virus H1N1 through Modulation of Protein Expression.

Kimchi, a traditional fermented food regularly consumed in Korea, contains various types of antimicrobial compounds. Among the tested compounds present in common spices used in Kimchi, quercetin showed the highest selectivity index against influenza A virus (IAV) H1N1. In this study, the effect of pretreatment and periodic treatment with quercetin against IAV in Madin-Darby canine kidney cells was observed. Compared to pretreatment, periodic treatment resulted in significantly higher cell viability but lower relative expression of the IAV PA gene and total apoptosis and cell death. To explain the mechanisms underlying the antiviral effects of quercetin treatment, a comparative proteomic analysis was performed in four samples (mock, quercetin-treated, IAV-infected, and quercetin-treated IAV-infected). Among the 220 proteins, 56 proteins were classified nonhierarchically into three clusters and were differentially modulated by quercetin treatment in IAV-infected cells. Post-translational modifications were identified in 68 proteins. In conclusion, periodic treatment with quercetin is effective in reducing IAV infection, and differentially regulates the expression of key proteins, including heat shock proteins, fibronectin 1, and prohibitin to reduce IAV replication.

A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments.

Selective perturbation of the activity of specific cell types in the brain tissue is essential in understanding the function of neuronal circuits involved in cognition and behavior and might also provide therapeutic neuromodulation strategies. Such selective neuronal addressing can be achieved through the optical activation of light-sensitive proteins called opsins that are expressed in specific cell populations through genetic methods-hence the name "optogenetics." In optogenetic experiments, the electrical activity of the targeted cell populations is optically triggered and monitored using arrays of microelectrodes. In closed-loop studies, the optical stimulation parameters are adjusted based on the recorded activity, ideally in real time. Here we describe the basic tools and the protocols allowing closed-loop optogenic experiments in vivo.

Chewing ability and dental functional status.

PURPOSE: The aim of this research was to explore the relationship between chewing ability and dental functional status, perceived oral health-related quality of life, and a number of background variables in a Vietnamese population. MATERIALS AND METHODS: The cluster stratified sample consisted of 2,805 dentate subjects aged ⋝ 20 years from urban and rural areas in Southern Vietnam. Chewing ability was assessed using a questionnaire that included questions on perceived difficulty with respect to eight foods and three questions of the Oral Health Impact Profile that were considered relevant. RESULTS: Only a minority reported serious problems with chewing ability (reporting difficult or very difficult to chew). The logistic regression analysis on chewing ability outcomes showed significant relationships between chewing ability and having ⋝ 10 teeth in each arch, having sufficient molar regions (⋝ 1 molar posterior occlusal pair [POP] bilaterally) for hard and soft foods, and having sufficient premolar regions (⋝ 3 POPs), especially for hard foods. In the hierarchical functional classification system, likelihood to report complaints on chewing ability appeared to discriminate in the branch "⋝ 10 teeth in each arch." Likelihood at subsequent levels ranged from approximately 1.5 to 3. In the branch "< 10 teeth in each arch," likelihood did not discriminate because the groups lacked sufficient homogeneity. CONCLUSIONS: Chewing ability and oral health-related quality of life were positively correlated. Among all dental and other variables, decreased chewing ability was strongest when correlated with older age categories and not correlated or weakly correlated with sex, socioeconomic status, and residence.