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1.
Toxicon ; 239: 107606, 2024 Feb 23.
Article in English | MEDLINE | ID: mdl-38181837

ABSTRACT

Cyclotides, plant-derived cysteine-rich peptides, exhibit a wide range of beneficial biological activities and possess exceptional structural stability. Cyclotides are commonly distributed throughout the Violaceae family. Viola dalatensis Gagnep, a Vietnamese species, has not been well studied, especially for cyclotides. This pioneering research explores cyclotides from V. dalatensis as antimicrobials. This study used a novel approach to enhance cyclotides after extraction. The approach combined 30% ammonium sulfate salt precipitation and RP-HPLC. A comprehensive analysis was performed to ascertain the overall protein content, flavonoids content, polyphenol content, and free radical scavenging capacity of compounds derived from V. dalatensis. Six known cyclotides were sequenced utilizing MS tandem. Semi-purified cyclotide mixtures (M1, M2, and M3) exhibited antibacterial efficacy against Bacillus subtilis (inhibitory diameters: 19.67-23.50 mm), Pseudomonas aeruginosa (22.17-23.50 mm), and Aspergillus flavus (14.67-21.33 mm). The enriched cyclotide precipitate from the stem extract demonstrated a minimum inhibitory concentration (MIC) of 0.08 mg/mL against P. aeruginosa, showcasing significant antibacterial effectiveness compared to the stem extract (MIC: 12.50 mg/mL). Considerable advancements have been achieved in the realm of cyclotides, specifically in their application as antimicrobial agents.


Subject(s)
Cyclotides , Viola , Cyclotides/pharmacology , Cyclotides/chemistry , Viola/chemistry , Viola/metabolism , Plant Extracts/pharmacology , Plant Extracts/chemistry , Anti-Bacterial Agents/chemistry , Vietnam
2.
BMC Cell Biol ; 11: 57, 2010 Jul 20.
Article in English | MEDLINE | ID: mdl-20646279

ABSTRACT

BACKGROUND: In normal adult skeletal muscle, cell turnover is very slow. However, after an acute lesion or in chronic pathological conditions, such as primary myopathies, muscle stem cells, called satellite cells, are induced to proliferate, then withdraw definitively from the cell cycle and fuse to reconstitute functional myofibers. RESULTS: We show that Maged1 is expressed at very low levels in normal adult muscle but is strongly induced after injury, during the early phase of myoblast differentiation. By comparing in vitro differentiation of myoblasts derived from wild-type or Maged1 knockout mice, we observed that Maged1 deficiency results in reduced levels of p21CIP1/WAF1, defective cell cycle exit and impaired myotube maturation. In vivo, this defect results in delayed regeneration of injured muscle. CONCLUSIONS: These data demonstrate for the first time that Maged1 is an important factor required for proper skeletal myoblast differentiation and muscle healing.


Subject(s)
Cyclin-Dependent Kinase Inhibitor p21/metabolism , Muscle, Skeletal/metabolism , Neoplasm Proteins/biosynthesis , Animals , Cell Cycle/genetics , Cell Line , Cyclin-Dependent Kinase Inhibitor p21/genetics , Mice , Mice, Knockout , Mice, Mutant Strains , Muscle Development , Muscle Fibers, Skeletal/metabolism , Muscle, Skeletal/injuries , Muscle, Skeletal/pathology , Muscular Diseases/pathology , Muscular Diseases/physiopathology , Neoplasm Proteins/genetics , Regeneration , Satellite Cells, Skeletal Muscle
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