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1.
Genet Mol Res ; 13(4): 8971-81, 2014 Oct 31.
Article in English | MEDLINE | ID: mdl-25366788

ABSTRACT

Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for MDIi-2 from healthy adult Muscovy Duck. The full-length MDIi-2 cDNA sequence was found to be 1377-base pairs, encoding a 285-amino acid protein. MDIi-2 contains 63 amino acids with an insertion sequence in the Tg domain. MDIi-2 shares high identity (72.51-94.74%) with the same protein in other birds. The Tg domain of MDIi-2 is highly conserved and showed relatively high identity (96.83%) among all tested birds. The molecular structure of the Tg domain supports this conservation. MDIi-2 expression was measured in various tissues using real-time quantitative polymerase chain reaction. Similar to MDIi-1, MDIi-2 was detected in all tissues but at different levels. Higher expression level was observed in the spleen, intestinal mucosa, and bursa stipe (bursa of Fabricius stipe) than in other tissues. This suggests that MDIi-2, like MDIi-1, plays an essential role in all tissues and that its differential expression may be related to its functions in these tissues. The coexistence of 2 MDIi isoforms indicates that their functions are correlated in Muscovy Duck. This study improves the understanding of poultry immunology and may be used to improve measures to protect Muscovy Duck from disease.


Subject(s)
Antigens, Differentiation, B-Lymphocyte/genetics , Ducks/genetics , Gene Expression Profiling , Histocompatibility Antigens Class II/genetics , Organ Specificity/genetics , Amino Acid Sequence , Animals , Antigens, Differentiation, B-Lymphocyte/chemistry , Antigens, Differentiation, B-Lymphocyte/metabolism , Base Sequence , Binding Sites/genetics , DNA/chemistry , DNA/genetics , Histocompatibility Antigens Class II/chemistry , Histocompatibility Antigens Class II/metabolism , Intestinal Mucosa/metabolism , Kidney/metabolism , Lung/metabolism , Models, Molecular , Molecular Sequence Data , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/metabolism , Protein Structure, Tertiary , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Spleen/metabolism , Thymus Gland/metabolism
2.
Genet Mol Res ; 10(4): 2867-80, 2011 Nov 22.
Article in English | MEDLINE | ID: mdl-22179959

ABSTRACT

The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the MDIi gene in various tissues by real-time quantitative PCR. A full-length cDNA sequence of MDIi was obtained, 1188-bp long, encoding a 222-amino acid protein. A comparison of the amino acid sequence of Ii between muscovy duck and other birds showed high similarity (66.3-95.3%). Characteristic functional domains found in Ii of other species, such as cytoplasmic domain, transmembrane domain, class II-associated Ii-derived peptide (CLIP) and trimerization domain, were identified in MDIi. Although all functional domains of Ii were found to be highly conserved, small differences in the CLIP sequence occur among the various species. Expression of MDIi was detected in all tissues at different levels. A higher expression level was found in the spleen, intestinal mucosa and the bursa stipe (bursa of Fabricius stipe) than other tissues. This tissue-specific expression suggests that MDIi plays an essential role in all tissues and differential expression may be a function of the innate structures and essential functions of these tissues.


Subject(s)
Antigens, Differentiation, B-Lymphocyte/genetics , Ducks/genetics , Histocompatibility Antigens Class II/genetics , Immunity, Innate/genetics , Amino Acid Sequence , Animals , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Differentiation, B-Lymphocyte/immunology , Avian Proteins/genetics , Avian Proteins/immunology , Cloning, Molecular , Conserved Sequence , DNA, Complementary/biosynthesis , Ducks/immunology , Ducks/metabolism , Histocompatibility Antigens Class II/immunology , Models, Molecular , Molecular Sequence Data , Organ Specificity , Phylogeny , Polymerase Chain Reaction/methods , Protein Structure, Tertiary , RNA, Messenger/analysis , RNA, Messenger/biosynthesis , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid
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