ABSTRACT
Hibiscus (Hibiscus syriacus L.) is known as a horticultural plant of great ornamental and medicinal value. However, the effect of NaCl stress on hibiscus seedlings is unclear. Little is known about H. syriacus 'Duede Brabaul' (DB) and H. syriacus 'Blueberry Smoothie' (BS). Here, the effects of solutions with different concentrations of NaCl on the organic osmolytes, ion accumulation, and antioxidant enzyme activity of hibiscus seedling leaves were determined. The results showed that the Na+/K+ ratio was imbalanced with increasing NaCl concentration, especially in BS (range 34% to 121%), which was more sensitive than DB (range 32% to 187%) under NaCl concentrations of 50 to 200 mM. To cope with the osmotic stress, the content of organic osmolytes increased significantly. Additionally, NaCl stress caused a large increase in O2·- and H2O2, and other reactive oxygen species (ROS), and antioxidant enzyme activity was significantly increased to remove excess ROS. The expression level of genes related to salt tolerance was significantly higher in DB than that in BS under different NaCl concentrations. Taken together, DB possessed a stronger tolerance to salt stress and the results suggest membrane stability, Na+/K+, H2O2, catalase and ascorbate peroxidase as salt tolerance biomarkers that can be used for gene transformation and breeding in future hibiscus research.
ABSTRACT
A number of strict lockdown measures were implemented in the areas most affected by COVID-19 in China, including Ji'nan city, from 24 January to 7 February 2020. Due to these forced restrictions, the pollution levels in cities across the country drastically decreased within just a few days. Since traffic pollution and industrial emissions are important factors affecting regional air quality, congestion has a significant impact on the environment. Therefore, using the aid of air quality data for six pollutants (PM10, PM2.5, SO2, NO2, CO and O3) from 11 monitoring stations (located in urban, suburban and urban-industrial regions) across Ji'nan, we employed the air quality index (AQI) to investigate the spatial pattern of air quality in the pre-COVID-19 (pre-COVID) and COVID-19-related lockdown (COVID lockdown) periods. The results showed that air quality significantly improved during the COVID lockdown period. Among the selected pollutants, compared to the corresponding pre-COVID levels, the greatest reduction was observed for the concentration of NO2 (54.02%), while the smallest reduction was observed for the concentration of SO2 (27.92%). The PM2.5 (38.73%), PM10 (44.92%) and CO (30.60%) levels also decreased during the COVID lockdown period; only the O3 concentration increased (37.42%) during this period. Overall, air quality improved by approximate improvements of 37.33% during the COVID lockdown period. Approximately 35.48%, 37.01% and 43.43% in the AQI were observed in urban, suburban and urban-industrial regions, respectively. Therefore, the AQI exhibited remarkable regional differences in Ji'nan. This study demonstrates the contributions of the transportation sector and local emissions to improving air quality in typical urban areas, and these research results can provide guidance for the further monitoring of air pollution in northern Chinese cities.
