ABSTRACT
The anti-allergic effect of berberine was evaluated in cellular and animal models of allergic responses. In this study, the results of the in vitro model of immunoglobulin (Ig) E-mediated mast cell degranulation showed that berberine significantly inhibited the release of ß-hexosaminidase (ß-HEX), histamine, IL-4 and TNF-α in rat basophilic leukemia cells (RBL-2H3 cells). Pretreatment with berberine prevented morphological changes in IgE-stimulated RBL-2H3 cells such as the recovery of an elongated shape. Pretreatment with berberine also suppressed the phosphorylation of antigen-induced Lyn, Syk, and Gab2, thus suppressing the downstream MAPK pathways. In the in vivo model of allergic responses, administration of berberine inhibited passive cutaneous anaphylaxis (PCA) in mice. The above results indicate berberine could suppress mast cell activation and allergic responses.
Subject(s)
Anti-Allergic Agents/therapeutic use , Berberine/therapeutic use , Hypersensitivity/drug therapy , Mast Cells/drug effects , Animals , Cell Degranulation , Cell Line , Disease Models, Animal , Histamine/metabolism , Humans , Immunoglobulin E/metabolism , Interleukin-4/metabolism , Male , Mast Cells/physiology , Mice , Passive Cutaneous Anaphylaxis/drug effects , Rats , Tumor Necrosis Factor-alpha/metabolism , beta-N-Acetylhexosaminidases/metabolismABSTRACT
Coptisine is one of the main components of isoquinoline alkaloids in the coptidis rhizome. The effect of coptisine on allergic rhinitis has not been investigated. In this study, we report the effects and mechanisms of coptisine using monoclonal anti-2,4,6-dinitrophenyl-immunoglobulin (Ig) E/human serum albumin (DNP-IgE/HSA)-stimulated rat basophilic leukemia cells (RBL-2H3 cells) in vitro and an ovalbumin (OVA)-induced allergic rhinitis (AR) in mice. The results showed that coptisine markedly decreased the levels of ß-hexosaminidase, histamine, interleukin (IL)-4, and tumor necrosis factor (TNF)-α. Coptisine also prevented morphological changes, such as restoring an elongated shape, inhibiting granule release on toluidine blue staining, and reorganizing inhibited filamentous actins (F-actin). Additionally, coptisine blocked the phosphorylation of phosphoinositide3-kinase (PI3K)/Akt (as known as protein kinase B(PKB)) in RBL-2H3 cell. Furthermore, the results showed that coptisine suppressed OVA-induced allergic rhinitis symptoms, such as nasal rubbing and OVA-specific IgE, and histamine, IL-4 and TNF-α levels in the serum of AR mice. These data suggested that coptisine should have inhibitory effects on the inflammatory responses of mast cells, and may be beneficial for the development of coptisine as a potential anti-allergic drug.
Subject(s)
Berberine/analogs & derivatives , Cell Degranulation/drug effects , Mast Cells/physiology , Ovalbumin/adverse effects , Rhinitis, Allergic/drug therapy , Animals , Berberine/administration & dosage , Berberine/pharmacology , Cell Line , Disease Models, Animal , Histamine/metabolism , Immunoglobulin E , Interleukin-4/metabolism , Mast Cells/drug effects , Mice , Phosphorylation/drug effects , Proto-Oncogene Proteins c-akt/metabolism , Rats , Rhinitis, Allergic/chemically induced , Tumor Necrosis Factor-alpha/metabolism , beta-N-Acetylhexosaminidases/metabolismABSTRACT
Most allergic reactions are induced by mast cell activation. Mast cells play vital roles in the pathogenesis of allergic diseases. Bisdemethoxycurcumin (BDMC), a natural curcuminoid, has potential anti-allergic effects. Hence, we explored the effect of BDMC on mast cell-mediated allergic diseases. The study proved that BDMC suppresses ß-hexosaminidase release, granule release, and membrane ruffling in monoclonal anti-2,4,6-dinitrophenyl-immunoglobulin (Ig) E/human serum albumin (DNP-IgE/HSA)-stimulated rat basophilic leukaemia cells (RBL-2H3 cells), and BDMC suppressed ovalbumin (OVA)-induced allergic rhinitis (AR) symptoms and OVA-specific IgE levels in AR mice. Furthermore, BDMC increased the survival of compound 48/80 anaphylaxis shock mice and elevated the decreased rectal temperature in OVA-induced active systemic anaphylaxis mice. These findings indicate that BDMC regulates the degranulation of mast cells, demonstrating its potential in the treatment of mast cell-induced allergic reactions.
Subject(s)
Curcumin/analogs & derivatives , Hypersensitivity/prevention & control , Mast Cells/drug effects , Anaphylaxis/chemically induced , Anaphylaxis/drug therapy , Animals , Cell Line , Curcumin/pharmacology , Curcumin/therapeutic use , Diarylheptanoids , Male , Mice , Mice, Inbred BALB C , Ovalbumin/toxicity , Rats , Rhinitis, Allergic/chemically induced , Rhinitis, Allergic/drug therapy , p-Methoxy-N-methylphenethylamine/toxicityABSTRACT
Currently, a variety of studies have demonstrated that green tea has anti-allergic properties, and the major polyphenolic compound, epigallocatechin gallate (EGCG), plays a significant role. Some research indicates that EGCG reduces the production and expression of allergy-related substances. Therefore, EGCG has a potential effect of reducing allergic rhinitis (AR). In this study, the effect of EGCG on allergic rhinitis in an ovalbumin (OVA)-induced mouse model was investigated. After administration of EGCG, the number of sneezes and the occurrence of nasal rubbing were significantly decreased, the concentrations of immunoglobulin E (IgE) and histamine were suppressed in AR mouse serum, the levels of interleukin (IL)-1ß, IL-4, and IL-6 were reduced in AR mice nasal lavage fluid (NLF), and the nasal mucosa mRNA and protein expression of cyclooxygenase 2 (COX-2), IL-1ß, IL-4, and IL-6 were inhibited. The data indicate that EGCG has a beneficial effect of reducing allergic rhinitis.
